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Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus

Proapoptotic activities. The 55 proanthocyanidin-rich fractions obtained from 11 fruits (5 fractions per fruit) were tested at 50 μg/mL (final concentration) on SW620 cells for their apoptosis induction properties after 24 h of incubation. Apoptosis yield was evaluated in cytometry by PS cell surface expression as described under Materials and Methods.
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fig1: Proapoptotic activities. The 55 proanthocyanidin-rich fractions obtained from 11 fruits (5 fractions per fruit) were tested at 50 μg/mL (final concentration) on SW620 cells for their apoptosis induction properties after 24 h of incubation. Apoptosis yield was evaluated in cytometry by PS cell surface expression as described under Materials and Methods.

Mentions: The obtained fractions were then evaluated for proapoptotic activities on SW620 cells (Figure 1) and compared to apple procyanidins (apple Pcy), a standard well described [35–37].


Impact of Procyanidins from Different Berries on Caspase 8 Activation in Colon Cancer.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD - Oxid Med Cell Longev (2015)

Proapoptotic activities. The 55 proanthocyanidin-rich fractions obtained from 11 fruits (5 fractions per fruit) were tested at 50 μg/mL (final concentration) on SW620 cells for their apoptosis induction properties after 24 h of incubation. Apoptosis yield was evaluated in cytometry by PS cell surface expression as described under Materials and Methods.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477188&req=5

fig1: Proapoptotic activities. The 55 proanthocyanidin-rich fractions obtained from 11 fruits (5 fractions per fruit) were tested at 50 μg/mL (final concentration) on SW620 cells for their apoptosis induction properties after 24 h of incubation. Apoptosis yield was evaluated in cytometry by PS cell surface expression as described under Materials and Methods.
Mentions: The obtained fractions were then evaluated for proapoptotic activities on SW620 cells (Figure 1) and compared to apple procyanidins (apple Pcy), a standard well described [35–37].

Bottom Line: Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection.Lowbush blueberry extract triggers the strongest activity.We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire d'Innovation Thérapeutique, UMR 7200, Faculté de Pharmacie, Université de Strasbourg, 74 route du Rhin, BP 24, 67401 Illkirch Cedex, France.

ABSTRACT

Scope: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries.

Methods and results: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells.

Conclusion: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

No MeSH data available.


Related in: MedlinePlus