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In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

The electron-spray ionization (ESI) mass spectrum of 70% hydroalcoholic extract of Xanthosoma sagittifolium leaves. MS data were acquired in the negative ionization mode. The mass detector was an ion trap spectrometer equipped with an ESI interface. All data were analyzed using Bruker Daltonics ESI Compass Data Analysis version 4.0 SP1 (Bruker Daltonics Inc., MA, USA).
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fig7: The electron-spray ionization (ESI) mass spectrum of 70% hydroalcoholic extract of Xanthosoma sagittifolium leaves. MS data were acquired in the negative ionization mode. The mass detector was an ion trap spectrometer equipped with an ESI interface. All data were analyzed using Bruker Daltonics ESI Compass Data Analysis version 4.0 SP1 (Bruker Daltonics Inc., MA, USA).

Mentions: The chemical analysis of HEXs-L showed two substances with molecular weight of 563.1422 and 593.1530 and molecular formulae of C26H27O14 and C27H29O15, respectively, as major compounds, proposed as apigenin di-C-glycosides, as well as sucrose, pyroglutamic acid (5-oxoproline), and some fatty acids (palmitic, stearic, and linolenic acids) by ESI-MS (Figure 7).


In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

The electron-spray ionization (ESI) mass spectrum of 70% hydroalcoholic extract of Xanthosoma sagittifolium leaves. MS data were acquired in the negative ionization mode. The mass detector was an ion trap spectrometer equipped with an ESI interface. All data were analyzed using Bruker Daltonics ESI Compass Data Analysis version 4.0 SP1 (Bruker Daltonics Inc., MA, USA).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477105&req=5

fig7: The electron-spray ionization (ESI) mass spectrum of 70% hydroalcoholic extract of Xanthosoma sagittifolium leaves. MS data were acquired in the negative ionization mode. The mass detector was an ion trap spectrometer equipped with an ESI interface. All data were analyzed using Bruker Daltonics ESI Compass Data Analysis version 4.0 SP1 (Bruker Daltonics Inc., MA, USA).
Mentions: The chemical analysis of HEXs-L showed two substances with molecular weight of 563.1422 and 593.1530 and molecular formulae of C26H27O14 and C27H29O15, respectively, as major compounds, proposed as apigenin di-C-glycosides, as well as sucrose, pyroglutamic acid (5-oxoproline), and some fatty acids (palmitic, stearic, and linolenic acids) by ESI-MS (Figure 7).

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus