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In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Effects of HEXs-L on iron(II)-chelating activity by the ferrozine assay. Quercetin was used as a chelating activity positive control. The results express mean ± S.D. of three experiments with triplicates. There were no significant differences between HEXs-L and quercetin, by Student's t-test.
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fig5: Effects of HEXs-L on iron(II)-chelating activity by the ferrozine assay. Quercetin was used as a chelating activity positive control. The results express mean ± S.D. of three experiments with triplicates. There were no significant differences between HEXs-L and quercetin, by Student's t-test.

Mentions: The inflammation microenvironment, rich in reactive oxygen (ROS) and NO/peroxynitrite, and cancer promotion have been linked. The antioxidant property of HEXs-L was then investigated by ferrous iron-chelating ability, since Fe2+ can lead to hydroxyl radical (OH∙) production via Fenton reaction. The results (Figure 5) indicated a high ferrous iron-chelating ability for HEXs-L that did not differ significantly from that of quercetin, the positive control of ferrous iron-chelating activity used in this assay. HEXs-L also reduced NO production by RAW 264.7 macrophages in a concentration-dependent manner (Figure 6(a)), showing IC50 of 86.3 µg/mL, without cytotoxic effects (Figure 6(b)).


In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Effects of HEXs-L on iron(II)-chelating activity by the ferrozine assay. Quercetin was used as a chelating activity positive control. The results express mean ± S.D. of three experiments with triplicates. There were no significant differences between HEXs-L and quercetin, by Student's t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477105&req=5

fig5: Effects of HEXs-L on iron(II)-chelating activity by the ferrozine assay. Quercetin was used as a chelating activity positive control. The results express mean ± S.D. of three experiments with triplicates. There were no significant differences between HEXs-L and quercetin, by Student's t-test.
Mentions: The inflammation microenvironment, rich in reactive oxygen (ROS) and NO/peroxynitrite, and cancer promotion have been linked. The antioxidant property of HEXs-L was then investigated by ferrous iron-chelating ability, since Fe2+ can lead to hydroxyl radical (OH∙) production via Fenton reaction. The results (Figure 5) indicated a high ferrous iron-chelating ability for HEXs-L that did not differ significantly from that of quercetin, the positive control of ferrous iron-chelating activity used in this assay. HEXs-L also reduced NO production by RAW 264.7 macrophages in a concentration-dependent manner (Figure 6(a)), showing IC50 of 86.3 µg/mL, without cytotoxic effects (Figure 6(b)).

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus