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In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Effects of HEXs-L on Jurkat cell apoptosis by annexin-V-FITC labeling assay. (a) Early and late apoptotic cells (mean of three independent experiments). Cytograms of a representative experiment are shown in (b) control, (c) HEXs-L, and (d) MTX treated cultures. Cells (1 × 105/mL) were treated with HEXs-L (50 µg/mL) or MTX (2 µg/mL) or culture medium (control) for 48 h and then processed for flow cytometric analysis of apoptosis as described in Materials and Methods section. The results in (d) express mean ± S.D. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
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fig4: Effects of HEXs-L on Jurkat cell apoptosis by annexin-V-FITC labeling assay. (a) Early and late apoptotic cells (mean of three independent experiments). Cytograms of a representative experiment are shown in (b) control, (c) HEXs-L, and (d) MTX treated cultures. Cells (1 × 105/mL) were treated with HEXs-L (50 µg/mL) or MTX (2 µg/mL) or culture medium (control) for 48 h and then processed for flow cytometric analysis of apoptosis as described in Materials and Methods section. The results in (d) express mean ± S.D. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.

Mentions: Treatment with HEXs-L for 48 h inhibited Jurkat cell proliferation, reducing significantly (50.7%) the viable cell number, compared to control (Figure 3(a)), as observed with methotrexate- (MTX) (80.5%) treated cultures. This HEXs-L effect was accompanied by an increase (85.9%) of cells in the sub-G1 phase and a reduction (20.0%) of cells in the G2/M phase (Figure 3(b)). These effects in the cell cycle were also observed with the MTX treatment, although at higher level than the plant extract (Figure 4(b)). In addition, MTX increased cells in the G1 phase (13%) and reduced them in the S phase (38%), compared to HEXs-L, 85.9% and 20%, respectively, as noted above.


In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Effects of HEXs-L on Jurkat cell apoptosis by annexin-V-FITC labeling assay. (a) Early and late apoptotic cells (mean of three independent experiments). Cytograms of a representative experiment are shown in (b) control, (c) HEXs-L, and (d) MTX treated cultures. Cells (1 × 105/mL) were treated with HEXs-L (50 µg/mL) or MTX (2 µg/mL) or culture medium (control) for 48 h and then processed for flow cytometric analysis of apoptosis as described in Materials and Methods section. The results in (d) express mean ± S.D. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4477105&req=5

fig4: Effects of HEXs-L on Jurkat cell apoptosis by annexin-V-FITC labeling assay. (a) Early and late apoptotic cells (mean of three independent experiments). Cytograms of a representative experiment are shown in (b) control, (c) HEXs-L, and (d) MTX treated cultures. Cells (1 × 105/mL) were treated with HEXs-L (50 µg/mL) or MTX (2 µg/mL) or culture medium (control) for 48 h and then processed for flow cytometric analysis of apoptosis as described in Materials and Methods section. The results in (d) express mean ± S.D. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
Mentions: Treatment with HEXs-L for 48 h inhibited Jurkat cell proliferation, reducing significantly (50.7%) the viable cell number, compared to control (Figure 3(a)), as observed with methotrexate- (MTX) (80.5%) treated cultures. This HEXs-L effect was accompanied by an increase (85.9%) of cells in the sub-G1 phase and a reduction (20.0%) of cells in the G2/M phase (Figure 3(b)). These effects in the cell cycle were also observed with the MTX treatment, although at higher level than the plant extract (Figure 4(b)). In addition, MTX increased cells in the G1 phase (13%) and reduced them in the S phase (38%), compared to HEXs-L, 85.9% and 20%, respectively, as noted above.

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus