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In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus

Cytotoxic effects of HEXs-L on leukemia and normal cells. (a) Jurkat cells. (b) Nontumor NIH fibroblasts. Cells were incubated in the absence (control) or presence of HEXs-L for 48 h. Cytotoxicity was determined by the MTT assay. The results express mean percentage of control ± S.D. of three experiments with triplicates. ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
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fig2: Cytotoxic effects of HEXs-L on leukemia and normal cells. (a) Jurkat cells. (b) Nontumor NIH fibroblasts. Cells were incubated in the absence (control) or presence of HEXs-L for 48 h. Cytotoxicity was determined by the MTT assay. The results express mean percentage of control ± S.D. of three experiments with triplicates. ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.

Mentions: To investigate the effect of X. sagittifolium extracts on leukemia cells, the cytotoxicity of both leaf (HEXs-L) and rhizome (HEXs-R) extracts (50 µg/mL) was evaluated on lymphocytic (Jurkat) and myelocytic (K562) leukemia cells. The results, as shown in Figure 1, indicated that treatment for 48 h with HEXs-L partially inhibited mitochondrial reduction activity (MRA) of both leukemia cell lines, with Jurkat cells (Figure 1(a)) presenting higher inhibition index (33.6 ± 10.8%) than K562 cells (17.3 ± 7.5%) (Figure 1(b)). HEXs-R was not cytotoxic against leukemia cells. The effects of other HEXs-L concentrations (25–100 μg/mL) were evaluated as well, presenting IC50 of 95.9 μg/mL (Figure 2(a)). HEXs-L showed no cytotoxicity against NIH/3T3 fibroblasts (Figure 2(b)).


In Vitro Antileukemic Activity of Xanthosoma sagittifolium (Taioba) Leaf Extract.

Caxito ML, Correia RR, Gomes AC, Justo G, Coelho MG, Sakuragui CM, Kuster RM, Sabino KC - Evid Based Complement Alternat Med (2015)

Cytotoxic effects of HEXs-L on leukemia and normal cells. (a) Jurkat cells. (b) Nontumor NIH fibroblasts. Cells were incubated in the absence (control) or presence of HEXs-L for 48 h. Cytotoxicity was determined by the MTT assay. The results express mean percentage of control ± S.D. of three experiments with triplicates. ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477105&req=5

fig2: Cytotoxic effects of HEXs-L on leukemia and normal cells. (a) Jurkat cells. (b) Nontumor NIH fibroblasts. Cells were incubated in the absence (control) or presence of HEXs-L for 48 h. Cytotoxicity was determined by the MTT assay. The results express mean percentage of control ± S.D. of three experiments with triplicates. ∗∗∗p < 0.001, related to control, by one-way ANOVA followed by Tukey's posttest.
Mentions: To investigate the effect of X. sagittifolium extracts on leukemia cells, the cytotoxicity of both leaf (HEXs-L) and rhizome (HEXs-R) extracts (50 µg/mL) was evaluated on lymphocytic (Jurkat) and myelocytic (K562) leukemia cells. The results, as shown in Figure 1, indicated that treatment for 48 h with HEXs-L partially inhibited mitochondrial reduction activity (MRA) of both leukemia cell lines, with Jurkat cells (Figure 1(a)) presenting higher inhibition index (33.6 ± 10.8%) than K562 cells (17.3 ± 7.5%) (Figure 1(b)). HEXs-R was not cytotoxic against leukemia cells. The effects of other HEXs-L concentrations (25–100 μg/mL) were evaluated as well, presenting IC50 of 95.9 μg/mL (Figure 2(a)). HEXs-L showed no cytotoxicity against NIH/3T3 fibroblasts (Figure 2(b)).

Bottom Line: HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%.Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds.Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Bioquímica, IBRAG, Centro Biomédico, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro (UERJ), Avenida Professor Manoel de Abreu 44, PAPC, 4° Andar, 20550-170 Rio de Janeiro, RJ, Brazil ; Núcleo de Pesquisas de Produtos Naturais, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil.

ABSTRACT
Xanthosoma sagittifolium Schott is a herb of the Araceae family, popularly known as taioba, which is consumed as food in some regions of Brazil, Africa, and Asia. This species has already been evaluated for the antifungal activities. However, based on its potential antitumor activity, the present study further aimed to examine the antitumor, as well as chelation, activity of X. sagittifolium leaf extract. Results showed that hydroethanolic extract of X. sagittifolium leaves (HEXs-L) exhibits cytotoxic effects against the immortalized line of human T-lymphocytic (Jurkat) and myelogenous (K562) leukemia cells, but not nontumor RAW 264.7 macrophages or NIH/3T3 fibroblasts. HEXs-L inhibited 50.3% of Jurkat cell proliferation, reducing by 20% cells in G2/M phase, but increasing cells in sub-G1 phase, thereby inducing apoptosis by 54%. In addition, HEXs-L inhibited NO production by 59%, as determined by Griess reaction, and chelated 93.8% of free Fe(II), as demonstrated by ferrozine assay. Phytochemical studies were carried out by ESI-MS, identifying apigenin di-C-glycosides as major compounds. Overall, this work revealed that leaf extract of Xanthosoma sagittifolium presented chelating activity and in vitro antitumor activity, arresting cell cycle and inducing apoptosis of leukemia cells, thus providing evidence that taioba leaves may have practical application in cancer therapy.

No MeSH data available.


Related in: MedlinePlus