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Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effect of avocado oil in brain mitochondria ROS levels. Experiment was carried out using 10 mM glutamate/malate as a mitochondria ETC substrate. ROS levels were expressed in fluorescence arbitrary units (a.u.). Data are the mean ± EE of (n = 5-6). ∗p < 0.05 compared with brain control mitochondria.
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fig4: Effect of avocado oil in brain mitochondria ROS levels. Experiment was carried out using 10 mM glutamate/malate as a mitochondria ETC substrate. ROS levels were expressed in fluorescence arbitrary units (a.u.). Data are the mean ± EE of (n = 5-6). ∗p < 0.05 compared with brain control mitochondria.

Mentions: In order to explore whether the impairment in both ΔΨm and respiration was related to enhanced ROS levels, we evaluated the changes in the fluorescence of H2DCFDA in response to the addition of glutamate/malate. The data presented in Figure 4 shows that ROS levels were increased by 64.5% in brain mitochondria from diabetic rats when compared to control rats. It was also observed that avocado oil administration fully prevented this effect in mitochondria from diabetic rats, while in mitochondria from control rats, avocado oil did not altered ROS levels.


Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Effect of avocado oil in brain mitochondria ROS levels. Experiment was carried out using 10 mM glutamate/malate as a mitochondria ETC substrate. ROS levels were expressed in fluorescence arbitrary units (a.u.). Data are the mean ± EE of (n = 5-6). ∗p < 0.05 compared with brain control mitochondria.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477098&req=5

fig4: Effect of avocado oil in brain mitochondria ROS levels. Experiment was carried out using 10 mM glutamate/malate as a mitochondria ETC substrate. ROS levels were expressed in fluorescence arbitrary units (a.u.). Data are the mean ± EE of (n = 5-6). ∗p < 0.05 compared with brain control mitochondria.
Mentions: In order to explore whether the impairment in both ΔΨm and respiration was related to enhanced ROS levels, we evaluated the changes in the fluorescence of H2DCFDA in response to the addition of glutamate/malate. The data presented in Figure 4 shows that ROS levels were increased by 64.5% in brain mitochondria from diabetic rats when compared to control rats. It was also observed that avocado oil administration fully prevented this effect in mitochondria from diabetic rats, while in mitochondria from control rats, avocado oil did not altered ROS levels.

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus