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Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effect of avocado oil in complexes I (a); II (b); III; (c) and IV (d) activities in brain mitochondria. Data are the mean ± EE of (n = 4–8). ∗p < 0.05, ∗∗p < 0.01 compared with brain control mitochondria.
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fig3: Effect of avocado oil in complexes I (a); II (b); III; (c) and IV (d) activities in brain mitochondria. Data are the mean ± EE of (n = 4–8). ∗p < 0.05, ∗∗p < 0.01 compared with brain control mitochondria.

Mentions: The activities of the complexes from the ETC are shown in Figure 3. Regarding complex I, no differences in activity (Figure 3(a)) were observed between the control and diabetic groups; however, avocado oil intake decreased 40.5% of this activity in comparison with the control group. Diabetes did not provoked changes in complex II activity (Figure 3(b)), but avocado oil intake in control rats induced an increase of 3.2-fold in this activity. Regarding the activity of the complex III (Figure 3(c)), diabetes enhanced this activity by 44.2% when compared to control group. Avocado oil also augmented the activity of complex III in mitochondria from diabetic and normoglycemic rats in 125.7% and 87.1% respectively. Complex IV activities were similar in the mitochondrial from all groups, except by the control group treated with avocado oil, which exhibited a decrease of 54.9% in complex IV activity.


Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Effect of avocado oil in complexes I (a); II (b); III; (c) and IV (d) activities in brain mitochondria. Data are the mean ± EE of (n = 4–8). ∗p < 0.05, ∗∗p < 0.01 compared with brain control mitochondria.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477098&req=5

fig3: Effect of avocado oil in complexes I (a); II (b); III; (c) and IV (d) activities in brain mitochondria. Data are the mean ± EE of (n = 4–8). ∗p < 0.05, ∗∗p < 0.01 compared with brain control mitochondria.
Mentions: The activities of the complexes from the ETC are shown in Figure 3. Regarding complex I, no differences in activity (Figure 3(a)) were observed between the control and diabetic groups; however, avocado oil intake decreased 40.5% of this activity in comparison with the control group. Diabetes did not provoked changes in complex II activity (Figure 3(b)), but avocado oil intake in control rats induced an increase of 3.2-fold in this activity. Regarding the activity of the complex III (Figure 3(c)), diabetes enhanced this activity by 44.2% when compared to control group. Avocado oil also augmented the activity of complex III in mitochondria from diabetic and normoglycemic rats in 125.7% and 87.1% respectively. Complex IV activities were similar in the mitochondrial from all groups, except by the control group treated with avocado oil, which exhibited a decrease of 54.9% in complex IV activity.

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus