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Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effect of avocado oil on mitochondrial membrane potential (ΔΨm). Representative traces of fresh isolated brain mitochondria from diabetic rat. Membrane potential was expressed in fluorescence arbitrary units (a.u.). The traces are typical of four experiments.
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fig2: Effect of avocado oil on mitochondrial membrane potential (ΔΨm). Representative traces of fresh isolated brain mitochondria from diabetic rat. Membrane potential was expressed in fluorescence arbitrary units (a.u.). The traces are typical of four experiments.

Mentions: Further characterization of impaired brain mitochondrial function in diabetic rats was carried out by analyzing ΔΨm. As observed in Figure 2, the energization of mitochondria from the control group (black line) with glutamate/malate elicited a large, instantaneous decrease in Safranin O fluorescence, which reflects the establishment of the ΔΨm. Moreover, ΔΨm remained stable after 2 min and the addition of an uncoupler (CCCP) induced an increase in Safranin fluorescence at initial levels before substrate addition, which is indicative of full dissipation of the ΔΨm. In contrast, the changes elicited by glutamate/malate in Safranin fluorescence were of a considerably lower magnitude in mitochondria from diabetic rats (gray line) and occurs at a slower rate than in mitochondria from control rats (black line), which together indicates that diabetes impaired brain mitochondrial functionality. Notably, avocado oil intake fully prevented the alterations in the ΔΨm observed in diabetic rats (gray pointed line) and did not alter this parameter in the control group (black discontinuous line). Therefore, these results confirm that diabetes induced brain mitochondrial dysfunction and that avocado oil fully prevented this alteration.


Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Effect of avocado oil on mitochondrial membrane potential (ΔΨm). Representative traces of fresh isolated brain mitochondria from diabetic rat. Membrane potential was expressed in fluorescence arbitrary units (a.u.). The traces are typical of four experiments.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477098&req=5

fig2: Effect of avocado oil on mitochondrial membrane potential (ΔΨm). Representative traces of fresh isolated brain mitochondria from diabetic rat. Membrane potential was expressed in fluorescence arbitrary units (a.u.). The traces are typical of four experiments.
Mentions: Further characterization of impaired brain mitochondrial function in diabetic rats was carried out by analyzing ΔΨm. As observed in Figure 2, the energization of mitochondria from the control group (black line) with glutamate/malate elicited a large, instantaneous decrease in Safranin O fluorescence, which reflects the establishment of the ΔΨm. Moreover, ΔΨm remained stable after 2 min and the addition of an uncoupler (CCCP) induced an increase in Safranin fluorescence at initial levels before substrate addition, which is indicative of full dissipation of the ΔΨm. In contrast, the changes elicited by glutamate/malate in Safranin fluorescence were of a considerably lower magnitude in mitochondria from diabetic rats (gray line) and occurs at a slower rate than in mitochondria from control rats (black line), which together indicates that diabetes impaired brain mitochondrial functionality. Notably, avocado oil intake fully prevented the alterations in the ΔΨm observed in diabetic rats (gray pointed line) and did not alter this parameter in the control group (black discontinuous line). Therefore, these results confirm that diabetes induced brain mitochondrial dysfunction and that avocado oil fully prevented this alteration.

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus