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Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus

Effects of avocado oil treatment in brain mitochondrial respiratory chain parameters: (a) state 3 respiration; (b) state 4 respiration, and (c) respiratory control ratio (RCR). Data are the mean ± EE of n = 5. ∗p < 0.05 compared with brain control mitochondria.
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fig1: Effects of avocado oil treatment in brain mitochondrial respiratory chain parameters: (a) state 3 respiration; (b) state 4 respiration, and (c) respiratory control ratio (RCR). Data are the mean ± EE of n = 5. ∗p < 0.05 compared with brain control mitochondria.

Mentions: Oxygen consumption rates (OCR) in resting state (state 4) and phosphorylating state (state 3) were measured with the objective to evaluate whether diabetes impaired mitochondrial function and determine the protective effects of avocado oil. Diabetes had a notable impact on brain mitochondria respiration (Figures 1(a) and 1(b)) as OCR in states 4 and 3 decreased 41.3% and 54.5%, respectively. Importantly, avocado oil prevented these effects, being this protective effect more prominent in state 3 respiration (Figure 1(b)). Impaired respiratory rates led to lower RCR in diabetic rats (Figure 1(c)), although, in this case, RCR values were not different in a statistically significant way.


Avocado Oil Improves Mitochondrial Function and Decreases Oxidative Stress in Brain of Diabetic Rats.

Ortiz-Avila O, Esquivel-Martínez M, Olmos-Orizaba BE, Saavedra-Molina A, Rodriguez-Orozco AR, Cortés-Rojo C - J Diabetes Res (2015)

Effects of avocado oil treatment in brain mitochondrial respiratory chain parameters: (a) state 3 respiration; (b) state 4 respiration, and (c) respiratory control ratio (RCR). Data are the mean ± EE of n = 5. ∗p < 0.05 compared with brain control mitochondria.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477098&req=5

fig1: Effects of avocado oil treatment in brain mitochondrial respiratory chain parameters: (a) state 3 respiration; (b) state 4 respiration, and (c) respiratory control ratio (RCR). Data are the mean ± EE of n = 5. ∗p < 0.05 compared with brain control mitochondria.
Mentions: Oxygen consumption rates (OCR) in resting state (state 4) and phosphorylating state (state 3) were measured with the objective to evaluate whether diabetes impaired mitochondrial function and determine the protective effects of avocado oil. Diabetes had a notable impact on brain mitochondria respiration (Figures 1(a) and 1(b)) as OCR in states 4 and 3 decreased 41.3% and 54.5%, respectively. Importantly, avocado oil prevented these effects, being this protective effect more prominent in state 3 respiration (Figure 1(b)). Impaired respiratory rates led to lower RCR in diabetic rats (Figure 1(c)), although, in this case, RCR values were not different in a statistically significant way.

Bottom Line: Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications.Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well.These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

View Article: PubMed Central - PubMed

Affiliation: Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, MICH, Mexico.

ABSTRACT
Diabetic encephalopathy is a diabetic complication related to the metabolic alterations featuring diabetes. Diabetes is characterized by increased lipid peroxidation, altered glutathione redox status, exacerbated levels of ROS, and mitochondrial dysfunction. Although the pathophysiology of diabetic encephalopathy remains to be clarified, oxidative stress and mitochondrial dysfunction play a crucial role in the pathogenesis of chronic diabetic complications. Taking this into consideration, the aim of this work was to evaluate the effects of 90-day avocado oil intake in brain mitochondrial function and oxidative status in streptozotocin-induced diabetic rats (STZ rats). Avocado oil improves brain mitochondrial function in diabetic rats preventing impairment of mitochondrial respiration and mitochondrial membrane potential (ΔΨ m ), besides increasing complex III activity. Avocado oil also decreased ROS levels and lipid peroxidation and improved the GSH/GSSG ratio as well. These results demonstrate that avocado oil supplementation prevents brain mitochondrial dysfunction induced by diabetes in association with decreased oxidative stress.

No MeSH data available.


Related in: MedlinePlus