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Proteases of Wood Rot Fungi with Emphasis on the Genus Pleurotus.

Inácio FD, Ferreira RO, de Araujo CA, Brugnari T, Castoldi R, Peralta RM, de Souza CG - Biomed Res Int (2015)

Bottom Line: The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation.There are few studies in the literature about proteases of Pleurotus spp.This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry of Microorganisms, Department of Biochemistry, State University of Maringá, Avenue Colombo 5790, 87015-900 Maringá, PR, Brazil ; Federal Institute of Paraná, Campus Jacarezinho, Avenue Doutor Tito s/n, Jardim Panorama, 86400-000 Jacarezinho, PR, Brazil.

ABSTRACT
Proteases are present in all living organisms and they play an important role in physiological conditions. Cell growth and death, blood clotting, and immune defense are all examples of the importance of proteases in maintaining homeostasis. There is growing interest in proteases due to their use for industrial purposes. The search for proteases with specific characteristics is designed to reduce production costs and to find suitable properties for certain industrial sectors, as well as good producing organisms. Ninety percent of commercialized proteases are obtained from microbial sources and proteases from macromycetes have recently gained prominence in the search for new enzymes with specific characteristics. The production of proteases from saprophytic basidiomycetes has led to the identification of various classes of proteases. The genus Pleurotus has been extensively studied because of its ligninolytic enzymes. The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation. There are few studies in the literature about proteases of Pleurotus spp. This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

No MeSH data available.


Ribbon model of subtilisin BPN (blue) from Bacillus amyloliquefaciens in complex with serine protease inhibitor POIA1 (red) and calcium ion (grey sphere). Figure from pdbid: 1V5I.
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fig2: Ribbon model of subtilisin BPN (blue) from Bacillus amyloliquefaciens in complex with serine protease inhibitor POIA1 (red) and calcium ion (grey sphere). Figure from pdbid: 1V5I.

Mentions: A recent study compared the genomes of 33 basidiomycetes and resulted in the idea that the division of fungi into white rot fungi and brown rot fungi in wood cannot be sustained because of the existence of DNA sequences shared between the two groups of fungi that attributes complexity to the mechanisms of degradation of cellulose, hemicellulose, and lignin by basidiomycetes [89]. The three-dimensional structures of proteases and their inhibitors provide rich information about the mechanisms involved in catalysis, and they suggest processes for enzyme inhibition that are still unknown. Using X-ray diffraction, the three-dimensional structure of a serine protease inhibitor of P. ostreatus complexed with subtilisin is shown in Figure 2 [84]. Table 1 shows some characteristics of proteases Pleurotus spp.


Proteases of Wood Rot Fungi with Emphasis on the Genus Pleurotus.

Inácio FD, Ferreira RO, de Araujo CA, Brugnari T, Castoldi R, Peralta RM, de Souza CG - Biomed Res Int (2015)

Ribbon model of subtilisin BPN (blue) from Bacillus amyloliquefaciens in complex with serine protease inhibitor POIA1 (red) and calcium ion (grey sphere). Figure from pdbid: 1V5I.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4477095&req=5

fig2: Ribbon model of subtilisin BPN (blue) from Bacillus amyloliquefaciens in complex with serine protease inhibitor POIA1 (red) and calcium ion (grey sphere). Figure from pdbid: 1V5I.
Mentions: A recent study compared the genomes of 33 basidiomycetes and resulted in the idea that the division of fungi into white rot fungi and brown rot fungi in wood cannot be sustained because of the existence of DNA sequences shared between the two groups of fungi that attributes complexity to the mechanisms of degradation of cellulose, hemicellulose, and lignin by basidiomycetes [89]. The three-dimensional structures of proteases and their inhibitors provide rich information about the mechanisms involved in catalysis, and they suggest processes for enzyme inhibition that are still unknown. Using X-ray diffraction, the three-dimensional structure of a serine protease inhibitor of P. ostreatus complexed with subtilisin is shown in Figure 2 [84]. Table 1 shows some characteristics of proteases Pleurotus spp.

Bottom Line: The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation.There are few studies in the literature about proteases of Pleurotus spp.This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Biochemistry of Microorganisms, Department of Biochemistry, State University of Maringá, Avenue Colombo 5790, 87015-900 Maringá, PR, Brazil ; Federal Institute of Paraná, Campus Jacarezinho, Avenue Doutor Tito s/n, Jardim Panorama, 86400-000 Jacarezinho, PR, Brazil.

ABSTRACT
Proteases are present in all living organisms and they play an important role in physiological conditions. Cell growth and death, blood clotting, and immune defense are all examples of the importance of proteases in maintaining homeostasis. There is growing interest in proteases due to their use for industrial purposes. The search for proteases with specific characteristics is designed to reduce production costs and to find suitable properties for certain industrial sectors, as well as good producing organisms. Ninety percent of commercialized proteases are obtained from microbial sources and proteases from macromycetes have recently gained prominence in the search for new enzymes with specific characteristics. The production of proteases from saprophytic basidiomycetes has led to the identification of various classes of proteases. The genus Pleurotus has been extensively studied because of its ligninolytic enzymes. The characteristics of this genus are easy cultivation techniques, high yield, low nutrient requirements, and excellent adaptation. There are few studies in the literature about proteases of Pleurotus spp. This review gathers together information about proteases, especially those derived from basidiomycetes, and aims at stimulating further research about fungal proteases because of their physiological importance and their application in various industries such as biotechnology and medicine.

No MeSH data available.