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Matured Hop Bittering Components Induce Thermogenesis in Brown Adipose Tissue via Sympathetic Nerve Activity.

Morimoto-Kobayashi Y, Ohara K, Takahashi C, Kitao S, Wang G, Taniguchi Y, Katayama M, Nagai K - PLoS ONE (2015)

Bottom Line: Therefore, we tested MHB for an effect on ameliorating diet-induced body fat accumulation in rodents.MHB ingestion had a beneficial effect but, compared to iso-α-acids and despite containing structurally similar compounds, acted via different mechanisms to reduce body fat accumulation.We also found that uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) was significantly increased in MHB-fed mice at both the mRNA and protein levels.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratories for Health Science & Food Technologies, KIRIN Company, Ltd., Yokohama, Kanagawa, Japan.

ABSTRACT
Obesity is the principal symptom of metabolic syndrome, which refers to a group of risk factors that increase the likelihood of atherosclerosis. In recent decades there has been a sharp rise in the incidence of obesity throughout the developed world. Iso-α-acids, the bitter compounds derived from hops in beer, have been shown to prevent diet-induced obesity by increasing lipid oxidation in the liver and inhibition of lipid absorption from the intestine. Whereas the sharp bitterness induced by effective dose of iso-α-acids precludes their acceptance as a nutrient, matured hop bittering components (MHB) appear to be more agreeable. Therefore, we tested MHB for an effect on ameliorating diet-induced body fat accumulation in rodents. MHB ingestion had a beneficial effect but, compared to iso-α-acids and despite containing structurally similar compounds, acted via different mechanisms to reduce body fat accumulation. MHB supplementation significantly reduced body weight gain, epididymal white adipose tissue weight, and plasma non-esterified free fatty acid levels in diet-induced obese mice. We also found that uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) was significantly increased in MHB-fed mice at both the mRNA and protein levels. In addition, MHB administration in rats induced the β-adrenergic signaling cascade, which is related to cAMP accumulation in BAT, suggesting that MHB could modulate sympathetic nerve activity innervating BAT (BAT-SNA). Indeed, single oral administration of MHB elevated BAT-SNA in rats, and this elevation was dissipated by subdiaphragmatic vagotomy. Single oral administration of MHB maintained BAT temperature at a significantly higher level than in control rats. Taken together, these findings indicate that MHB ameliorates diet-induced body fat accumulation, at least partly, by enhancing thermogenesis in BAT via BAT-SNA activation. Our data suggests that MHB is a useful tool for developing functional foods or beverages to counteract the accumulation of body fat.

No MeSH data available.


Related in: MedlinePlus

MHB ameliorated HFD-induced body fat accumulation in mice.(A) Body weight gain of HFD-fed mice with or without MHB supplementation. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by analysis of variance (ANOVA) with repeated measures). (B) Epididymal WAT, abdominal subcutaneous WAT and liver weight. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by unpaired Student’s t-test).
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pone.0131042.g001: MHB ameliorated HFD-induced body fat accumulation in mice.(A) Body weight gain of HFD-fed mice with or without MHB supplementation. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by analysis of variance (ANOVA) with repeated measures). (B) Epididymal WAT, abdominal subcutaneous WAT and liver weight. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by unpaired Student’s t-test).

Mentions: The body weight of mice fed HFD with and without MHB supplementation was monitored. As shown in Fig 1A, weight gain was significantly suppressed in the group fed a diet containing MHB compared to mice fed HFD without supplement (P < 0.01). There was no significant difference in food intake between the two groups (Table 1 and S3A Fig). Moreover, there was no significant correlation between total food intake and body weight gain (S3B Fig, Pearson correlation coefficient r = 0.21, p = 0.32). Epididymal white adipose tissue (WAT) weight of MHB-fed mice was also significantly decreased to 80.4% that of the control mice (Fig 1B, P < 0.01). There were no significant differences in plasma TG, total cholesterol and glucose levels between the two groups. However, the plasma NEFA level in mice fed HFD containing MHB was suppressed by 21.7% (P < 0.01) compared to that of the control group (Table 2). There was no significant difference in fecal excretion of lipids between the two groups (Table 1), suggesting that MHB does not affect the absorption rate of dietary fat under our experimental conditions.


Matured Hop Bittering Components Induce Thermogenesis in Brown Adipose Tissue via Sympathetic Nerve Activity.

Morimoto-Kobayashi Y, Ohara K, Takahashi C, Kitao S, Wang G, Taniguchi Y, Katayama M, Nagai K - PLoS ONE (2015)

MHB ameliorated HFD-induced body fat accumulation in mice.(A) Body weight gain of HFD-fed mice with or without MHB supplementation. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by analysis of variance (ANOVA) with repeated measures). (B) Epididymal WAT, abdominal subcutaneous WAT and liver weight. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by unpaired Student’s t-test).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476742&req=5

pone.0131042.g001: MHB ameliorated HFD-induced body fat accumulation in mice.(A) Body weight gain of HFD-fed mice with or without MHB supplementation. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by analysis of variance (ANOVA) with repeated measures). (B) Epididymal WAT, abdominal subcutaneous WAT and liver weight. Data are presented as means ± SEM. n = 12 mice/group. **P < 0.01 (by unpaired Student’s t-test).
Mentions: The body weight of mice fed HFD with and without MHB supplementation was monitored. As shown in Fig 1A, weight gain was significantly suppressed in the group fed a diet containing MHB compared to mice fed HFD without supplement (P < 0.01). There was no significant difference in food intake between the two groups (Table 1 and S3A Fig). Moreover, there was no significant correlation between total food intake and body weight gain (S3B Fig, Pearson correlation coefficient r = 0.21, p = 0.32). Epididymal white adipose tissue (WAT) weight of MHB-fed mice was also significantly decreased to 80.4% that of the control mice (Fig 1B, P < 0.01). There were no significant differences in plasma TG, total cholesterol and glucose levels between the two groups. However, the plasma NEFA level in mice fed HFD containing MHB was suppressed by 21.7% (P < 0.01) compared to that of the control group (Table 2). There was no significant difference in fecal excretion of lipids between the two groups (Table 1), suggesting that MHB does not affect the absorption rate of dietary fat under our experimental conditions.

Bottom Line: Therefore, we tested MHB for an effect on ameliorating diet-induced body fat accumulation in rodents.MHB ingestion had a beneficial effect but, compared to iso-α-acids and despite containing structurally similar compounds, acted via different mechanisms to reduce body fat accumulation.We also found that uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) was significantly increased in MHB-fed mice at both the mRNA and protein levels.

View Article: PubMed Central - PubMed

Affiliation: Research Laboratories for Health Science & Food Technologies, KIRIN Company, Ltd., Yokohama, Kanagawa, Japan.

ABSTRACT
Obesity is the principal symptom of metabolic syndrome, which refers to a group of risk factors that increase the likelihood of atherosclerosis. In recent decades there has been a sharp rise in the incidence of obesity throughout the developed world. Iso-α-acids, the bitter compounds derived from hops in beer, have been shown to prevent diet-induced obesity by increasing lipid oxidation in the liver and inhibition of lipid absorption from the intestine. Whereas the sharp bitterness induced by effective dose of iso-α-acids precludes their acceptance as a nutrient, matured hop bittering components (MHB) appear to be more agreeable. Therefore, we tested MHB for an effect on ameliorating diet-induced body fat accumulation in rodents. MHB ingestion had a beneficial effect but, compared to iso-α-acids and despite containing structurally similar compounds, acted via different mechanisms to reduce body fat accumulation. MHB supplementation significantly reduced body weight gain, epididymal white adipose tissue weight, and plasma non-esterified free fatty acid levels in diet-induced obese mice. We also found that uncoupling protein 1 (UCP1) expression in brown adipose tissue (BAT) was significantly increased in MHB-fed mice at both the mRNA and protein levels. In addition, MHB administration in rats induced the β-adrenergic signaling cascade, which is related to cAMP accumulation in BAT, suggesting that MHB could modulate sympathetic nerve activity innervating BAT (BAT-SNA). Indeed, single oral administration of MHB elevated BAT-SNA in rats, and this elevation was dissipated by subdiaphragmatic vagotomy. Single oral administration of MHB maintained BAT temperature at a significantly higher level than in control rats. Taken together, these findings indicate that MHB ameliorates diet-induced body fat accumulation, at least partly, by enhancing thermogenesis in BAT via BAT-SNA activation. Our data suggests that MHB is a useful tool for developing functional foods or beverages to counteract the accumulation of body fat.

No MeSH data available.


Related in: MedlinePlus