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Preferential Accumulation of 14C-N-Glycolylneuraminic Acid over 14C-N-Acetylneuraminic Acid in the Rat Brain after Tail Vein Injection.

Taguchi R, Minami A, Matsuda Y, Takahashi T, Otsubo T, Ikeda K, Suzuki T - PLoS ONE (2015)

Bottom Line: Brain autoradiography indicated that 14C-Neu5Gc was accumulated predominantly in the hippocampus. 14C-Neu5Gc transferred into the brain was incorporated into gangliosides including GM1, GD1a, GD1b, GT1b and GQ1b.Reduction of 14C-Neu5Gc after intracerebroventricular infusion was slower than that of 14C-Neu5Ac in the brain and hippocampus.The results suggest that Neu5Gc is transferred from blood into the brain across the blood brain barrier and accumulates in the brain more preferentially than does Neu5Ac.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.

ABSTRACT
The two main molecular species of sialic acid existing in nature are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). Neu5Ac is abundant in mammalian brains and plays crucial roles in many neural functions. In contrast, Neu5Gc is present only at a trace level in vertebrate brains. The brain-specific suppression of Neu5Gc synthesis, which is a common feature in mammals, suggests that Neu5Gc has toxicity against brain functions. However, in vivo kinetics of Neu5Gc in the whole body, especially in the brain, has not been studied in sufficient detail. To determine the in vivo kinetics of Neu5Gc, 14C-Neu5Gc was enzymatically synthesized and injected into rat tail veins. Although most of 14C-Neu5Gc was excreted in urine, a small amount of 14C-Neu5Gc was detected in the brain. Brain autoradiography indicated that 14C-Neu5Gc was accumulated predominantly in the hippocampus. 14C-Neu5Gc transferred into the brain was incorporated into gangliosides including GM1, GD1a, GD1b, GT1b and GQ1b. Reduction of 14C-Neu5Gc after intracerebroventricular infusion was slower than that of 14C-Neu5Ac in the brain and hippocampus. The results suggest that Neu5Gc is transferred from blood into the brain across the blood brain barrier and accumulates in the brain more preferentially than does Neu5Ac.

No MeSH data available.


Related in: MedlinePlus

In vivo kinetics of 14C-Neu5Gc and 14C-Neu5Ac in the whole body after tail vein injection.Radioactivity in each organ at 1, 3 and 24 hr after tail vein injection of 14C-Neu5Gc (A: n = 5) or 14C-Neu5Ac (B: n = 5–6) is shown as a relative value to the radioactivities of injected-14C-Neu5Gc or 14C-Neu5Ac.
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pone.0131061.g002: In vivo kinetics of 14C-Neu5Gc and 14C-Neu5Ac in the whole body after tail vein injection.Radioactivity in each organ at 1, 3 and 24 hr after tail vein injection of 14C-Neu5Gc (A: n = 5) or 14C-Neu5Ac (B: n = 5–6) is shown as a relative value to the radioactivities of injected-14C-Neu5Gc or 14C-Neu5Ac.

Mentions: To determine the in vivo kinetics of Neu5Gc in the rat whole body, the distribution of 14C-Neu5Gc was investigated after 14C-Neu5Gc injection into a tail vein. Most of the radioactivity was detected in urine (Fig 2A). Radioactivity in the blood and organs including the kidney, liver, lung, spleen and heart was detected at 1 hr after 14C-Neu5Gc injection and decreased over a period of 24 hr. Radioactivity was also detected in the brain after tail vein injection of 14C-Neu5Gc (Fig 2A). The whole body distribution of radioactivity in the 14C-Neu5Gc-injected rat was similar to that in the 14C-Neu5Ac-injected rat (Fig 2B).


Preferential Accumulation of 14C-N-Glycolylneuraminic Acid over 14C-N-Acetylneuraminic Acid in the Rat Brain after Tail Vein Injection.

Taguchi R, Minami A, Matsuda Y, Takahashi T, Otsubo T, Ikeda K, Suzuki T - PLoS ONE (2015)

In vivo kinetics of 14C-Neu5Gc and 14C-Neu5Ac in the whole body after tail vein injection.Radioactivity in each organ at 1, 3 and 24 hr after tail vein injection of 14C-Neu5Gc (A: n = 5) or 14C-Neu5Ac (B: n = 5–6) is shown as a relative value to the radioactivities of injected-14C-Neu5Gc or 14C-Neu5Ac.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476740&req=5

pone.0131061.g002: In vivo kinetics of 14C-Neu5Gc and 14C-Neu5Ac in the whole body after tail vein injection.Radioactivity in each organ at 1, 3 and 24 hr after tail vein injection of 14C-Neu5Gc (A: n = 5) or 14C-Neu5Ac (B: n = 5–6) is shown as a relative value to the radioactivities of injected-14C-Neu5Gc or 14C-Neu5Ac.
Mentions: To determine the in vivo kinetics of Neu5Gc in the rat whole body, the distribution of 14C-Neu5Gc was investigated after 14C-Neu5Gc injection into a tail vein. Most of the radioactivity was detected in urine (Fig 2A). Radioactivity in the blood and organs including the kidney, liver, lung, spleen and heart was detected at 1 hr after 14C-Neu5Gc injection and decreased over a period of 24 hr. Radioactivity was also detected in the brain after tail vein injection of 14C-Neu5Gc (Fig 2A). The whole body distribution of radioactivity in the 14C-Neu5Gc-injected rat was similar to that in the 14C-Neu5Ac-injected rat (Fig 2B).

Bottom Line: Brain autoradiography indicated that 14C-Neu5Gc was accumulated predominantly in the hippocampus. 14C-Neu5Gc transferred into the brain was incorporated into gangliosides including GM1, GD1a, GD1b, GT1b and GQ1b.Reduction of 14C-Neu5Gc after intracerebroventricular infusion was slower than that of 14C-Neu5Ac in the brain and hippocampus.The results suggest that Neu5Gc is transferred from blood into the brain across the blood brain barrier and accumulates in the brain more preferentially than does Neu5Ac.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.

ABSTRACT
The two main molecular species of sialic acid existing in nature are N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc). Neu5Ac is abundant in mammalian brains and plays crucial roles in many neural functions. In contrast, Neu5Gc is present only at a trace level in vertebrate brains. The brain-specific suppression of Neu5Gc synthesis, which is a common feature in mammals, suggests that Neu5Gc has toxicity against brain functions. However, in vivo kinetics of Neu5Gc in the whole body, especially in the brain, has not been studied in sufficient detail. To determine the in vivo kinetics of Neu5Gc, 14C-Neu5Gc was enzymatically synthesized and injected into rat tail veins. Although most of 14C-Neu5Gc was excreted in urine, a small amount of 14C-Neu5Gc was detected in the brain. Brain autoradiography indicated that 14C-Neu5Gc was accumulated predominantly in the hippocampus. 14C-Neu5Gc transferred into the brain was incorporated into gangliosides including GM1, GD1a, GD1b, GT1b and GQ1b. Reduction of 14C-Neu5Gc after intracerebroventricular infusion was slower than that of 14C-Neu5Ac in the brain and hippocampus. The results suggest that Neu5Gc is transferred from blood into the brain across the blood brain barrier and accumulates in the brain more preferentially than does Neu5Ac.

No MeSH data available.


Related in: MedlinePlus