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Non Digestible Oligosaccharides Modulate the Gut Microbiota to Control the Development of Leukemia and Associated Cachexia in Mice.

Bindels LB, Neyrinck AM, Salazar N, Taminiau B, Druart C, Muccioli GG, François E, Blecker C, Richel A, Daube G, Mahillon J, de los Reyes-Gavilán CG, Cani PD, Delzenne NM - PLoS ONE (2015)

Bottom Line: INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver.POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations.Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

View Article: PubMed Central - PubMed

Affiliation: Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.

ABSTRACT
We tested the hypothesis that changing the gut microbiota using pectic oligosaccharides (POS) or inulin (INU) differently modulates the progression of leukemia and related metabolic disorders. Mice were transplanted with Bcr-Abl-transfected proB lymphocytes mimicking leukemia and received either POS or INU in their diet (5%) for 2 weeks. Combination of pyrosequencing, PCR-DGGE and qPCR analyses of the 16S rRNA gene revealed that POS decreased microbial diversity and richness of caecal microbiota whereas it increased Bifidobacterium spp., Roseburia spp. and Bacteroides spp. (affecting specifically B. dorei) to a higher extent than INU. INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver. POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations. Indeed, POS better than INU delayed anorexia linked to cancer progression. In addition, POS treatment increased acetate in the caecal content, changed the fatty acid profile inside adipose tissue and counteracted the induction of markers controlling β-oxidation, thereby hampering fat mass loss. Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

No MeSH data available.


Related in: MedlinePlus

Caloric intake and fat mass evolution.Total caloric intake from the day of BaF inoculation to the necropsy (day 15) (A). Daily caloric intake per mice from day 11 to day 15 (B). Evolution of fat mass (C). Data with different superscript letters are significantly different.
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pone.0131009.g004: Caloric intake and fat mass evolution.Total caloric intake from the day of BaF inoculation to the necropsy (day 15) (A). Daily caloric intake per mice from day 11 to day 15 (B). Evolution of fat mass (C). Data with different superscript letters are significantly different.

Mentions: The total caloric intake was lower in BaF mice than CT mice (Fig 4A). The BaF group exhibited a reduced food intake from day 11 to day 15 (Fig 4B). Anorexia induced by the transplantation of BaF cells was accompanied by a loss of total fat mass linked to cancer progression (Fig 4C). Supplementation with POS significantly delayed the fall of food intake to a higher extent than INU (Fig 4A and 4B). Moreover, POS treatment reduced fat mass loss by about 50% (Fig 4C), whereas INU supplementation had no effect on fat mass. BaF transplantation decreased adipose tissue weight (subcutaneous and visceral) without affecting the muscle weight (soleus and gastrocnemius) (Table 1) in accordance with the well-established kinetics of this model [13]. POS treatment partly maintained adiposity, confirming the total fat mass analysis performed on live animals.


Non Digestible Oligosaccharides Modulate the Gut Microbiota to Control the Development of Leukemia and Associated Cachexia in Mice.

Bindels LB, Neyrinck AM, Salazar N, Taminiau B, Druart C, Muccioli GG, François E, Blecker C, Richel A, Daube G, Mahillon J, de los Reyes-Gavilán CG, Cani PD, Delzenne NM - PLoS ONE (2015)

Caloric intake and fat mass evolution.Total caloric intake from the day of BaF inoculation to the necropsy (day 15) (A). Daily caloric intake per mice from day 11 to day 15 (B). Evolution of fat mass (C). Data with different superscript letters are significantly different.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476728&req=5

pone.0131009.g004: Caloric intake and fat mass evolution.Total caloric intake from the day of BaF inoculation to the necropsy (day 15) (A). Daily caloric intake per mice from day 11 to day 15 (B). Evolution of fat mass (C). Data with different superscript letters are significantly different.
Mentions: The total caloric intake was lower in BaF mice than CT mice (Fig 4A). The BaF group exhibited a reduced food intake from day 11 to day 15 (Fig 4B). Anorexia induced by the transplantation of BaF cells was accompanied by a loss of total fat mass linked to cancer progression (Fig 4C). Supplementation with POS significantly delayed the fall of food intake to a higher extent than INU (Fig 4A and 4B). Moreover, POS treatment reduced fat mass loss by about 50% (Fig 4C), whereas INU supplementation had no effect on fat mass. BaF transplantation decreased adipose tissue weight (subcutaneous and visceral) without affecting the muscle weight (soleus and gastrocnemius) (Table 1) in accordance with the well-established kinetics of this model [13]. POS treatment partly maintained adiposity, confirming the total fat mass analysis performed on live animals.

Bottom Line: INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver.POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations.Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

View Article: PubMed Central - PubMed

Affiliation: Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.

ABSTRACT
We tested the hypothesis that changing the gut microbiota using pectic oligosaccharides (POS) or inulin (INU) differently modulates the progression of leukemia and related metabolic disorders. Mice were transplanted with Bcr-Abl-transfected proB lymphocytes mimicking leukemia and received either POS or INU in their diet (5%) for 2 weeks. Combination of pyrosequencing, PCR-DGGE and qPCR analyses of the 16S rRNA gene revealed that POS decreased microbial diversity and richness of caecal microbiota whereas it increased Bifidobacterium spp., Roseburia spp. and Bacteroides spp. (affecting specifically B. dorei) to a higher extent than INU. INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver. POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations. Indeed, POS better than INU delayed anorexia linked to cancer progression. In addition, POS treatment increased acetate in the caecal content, changed the fatty acid profile inside adipose tissue and counteracted the induction of markers controlling β-oxidation, thereby hampering fat mass loss. Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

No MeSH data available.


Related in: MedlinePlus