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Non Digestible Oligosaccharides Modulate the Gut Microbiota to Control the Development of Leukemia and Associated Cachexia in Mice.

Bindels LB, Neyrinck AM, Salazar N, Taminiau B, Druart C, Muccioli GG, François E, Blecker C, Richel A, Daube G, Mahillon J, de los Reyes-Gavilán CG, Cani PD, Delzenne NM - PLoS ONE (2015)

Bottom Line: INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver.POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations.Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

View Article: PubMed Central - PubMed

Affiliation: Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.

ABSTRACT
We tested the hypothesis that changing the gut microbiota using pectic oligosaccharides (POS) or inulin (INU) differently modulates the progression of leukemia and related metabolic disorders. Mice were transplanted with Bcr-Abl-transfected proB lymphocytes mimicking leukemia and received either POS or INU in their diet (5%) for 2 weeks. Combination of pyrosequencing, PCR-DGGE and qPCR analyses of the 16S rRNA gene revealed that POS decreased microbial diversity and richness of caecal microbiota whereas it increased Bifidobacterium spp., Roseburia spp. and Bacteroides spp. (affecting specifically B. dorei) to a higher extent than INU. INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver. POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations. Indeed, POS better than INU delayed anorexia linked to cancer progression. In addition, POS treatment increased acetate in the caecal content, changed the fatty acid profile inside adipose tissue and counteracted the induction of markers controlling β-oxidation, thereby hampering fat mass loss. Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

No MeSH data available.


Related in: MedlinePlus

Short chain fatty acid (SCFA) profile in the caecal content.Acetate (A). Propionate (B). Butyrate (C). Valerate (D). Isovalerate (E). Isobutyrate (F). Caproate (G). Branched SCFA (H). Total SCFAs (I). Data with different superscript letters are significantly different.
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pone.0131009.g003: Short chain fatty acid (SCFA) profile in the caecal content.Acetate (A). Propionate (B). Butyrate (C). Valerate (D). Isovalerate (E). Isobutyrate (F). Caproate (G). Branched SCFA (H). Total SCFAs (I). Data with different superscript letters are significantly different.

Mentions: Transplantation of BaF3 cells in mice did not modify levels of SCFA in the caecal content (Fig 3) or in the portal blood (Table 1). Supplementation with INU induced minor changes of SCFA in the caecum, namely a reduction in isovalerate (Fig 3E). However, it almost doubled propionate and butyrate levels in the portal blood (Table 1). POS increased acetate and lowered isovalerate in the caecal content, but did not significantly modify portal SCFA (Fig 3A and 3E, and Table 1). Both POS and INU reduced caecal branched SCFA, which are associated with negative end products of protein fermentation [4].


Non Digestible Oligosaccharides Modulate the Gut Microbiota to Control the Development of Leukemia and Associated Cachexia in Mice.

Bindels LB, Neyrinck AM, Salazar N, Taminiau B, Druart C, Muccioli GG, François E, Blecker C, Richel A, Daube G, Mahillon J, de los Reyes-Gavilán CG, Cani PD, Delzenne NM - PLoS ONE (2015)

Short chain fatty acid (SCFA) profile in the caecal content.Acetate (A). Propionate (B). Butyrate (C). Valerate (D). Isovalerate (E). Isobutyrate (F). Caproate (G). Branched SCFA (H). Total SCFAs (I). Data with different superscript letters are significantly different.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476728&req=5

pone.0131009.g003: Short chain fatty acid (SCFA) profile in the caecal content.Acetate (A). Propionate (B). Butyrate (C). Valerate (D). Isovalerate (E). Isobutyrate (F). Caproate (G). Branched SCFA (H). Total SCFAs (I). Data with different superscript letters are significantly different.
Mentions: Transplantation of BaF3 cells in mice did not modify levels of SCFA in the caecal content (Fig 3) or in the portal blood (Table 1). Supplementation with INU induced minor changes of SCFA in the caecum, namely a reduction in isovalerate (Fig 3E). However, it almost doubled propionate and butyrate levels in the portal blood (Table 1). POS increased acetate and lowered isovalerate in the caecal content, but did not significantly modify portal SCFA (Fig 3A and 3E, and Table 1). Both POS and INU reduced caecal branched SCFA, which are associated with negative end products of protein fermentation [4].

Bottom Line: INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver.POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations.Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

View Article: PubMed Central - PubMed

Affiliation: Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Brussels, Belgium.

ABSTRACT
We tested the hypothesis that changing the gut microbiota using pectic oligosaccharides (POS) or inulin (INU) differently modulates the progression of leukemia and related metabolic disorders. Mice were transplanted with Bcr-Abl-transfected proB lymphocytes mimicking leukemia and received either POS or INU in their diet (5%) for 2 weeks. Combination of pyrosequencing, PCR-DGGE and qPCR analyses of the 16S rRNA gene revealed that POS decreased microbial diversity and richness of caecal microbiota whereas it increased Bifidobacterium spp., Roseburia spp. and Bacteroides spp. (affecting specifically B. dorei) to a higher extent than INU. INU supplementation increased the portal SCFA propionate and butyrate, and decreased cancer cell invasion in the liver. POS treatment did not affect hepatic cancer cell invasion, but was more efficient than INU to decrease the metabolic alterations. Indeed, POS better than INU delayed anorexia linked to cancer progression. In addition, POS treatment increased acetate in the caecal content, changed the fatty acid profile inside adipose tissue and counteracted the induction of markers controlling β-oxidation, thereby hampering fat mass loss. Non digestible carbohydrates with prebiotic properties may constitute a new nutritional strategy to modulate gut microbiota with positive consequences on cancer progression and associated cachexia.

No MeSH data available.


Related in: MedlinePlus