Limits...
64Cu-ATSM Hypoxia Positron Emission Tomography for Detection of Conduit Ischemia in an Experimental Rat Esophagectomy Model.

Park SY, Kang WJ, Cho A, Chae JR, Cho YL, Kim JY, Lee JW, Chung KY - PLoS ONE (2015)

Bottom Line: Upon autoradiography, 64Cu-ATSM uptake was observed in the fundus in the operation group, and was well-correlated to that observed on the PET image.Upon immunohistochemistry, expression of hypoxia-inducible factor 1a and pimonidazole were significantly increased at the fundus and lesser curvature compared to the greater curvature in the operation group.Further clinical studies are needed to verify whether hypoxia imaging may be useful in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University, College of Medicine, Seoul, Republic of Korea; Department of Thoracic and Cardiovascular Surgery, Ajou University, School of Medicine, Suwon, Republic of Korea.

ABSTRACT

Background: We designed a hypoxia-imaging modality to detect ischemia of the gastric conduit after esophagectomy.

Materials and methods: A rat esophagectomy model was created using 12-16-week-old, 300-350 g male Sprague-Dawley rats. In the operation group (n=6), partial gastric devascularization was performed by ligating the left gastric artery and the short gastric arteries and an esophagogastric anastomosis was performed. In the control group (n=6), the esophageal-gastric junction was incised and suturing was performed without gastric devascularization. Positron emission tomography (PET) images were taken using a microPET rodent model scanner, 24 h after the initial operation, after injection of 200 μCi 64Cu-diacetyl-bis (N4-methylsemicarbazone) (64Cu-ATSM) and pimonidazole 120 mg/kg. After microPET imaging, autoradiography and immunohistochemistry were performed.

Results: The PET image revealed 64Cu-ATSM uptake at the fundus in the operation group 3 h after 64Cu-ATSM injection. The maximum percentage of the injected dose per gram of tissue was higher in the operation group (0.047±0.015 vs. 0.026±0.006, p=0.021). The fundus/liver ratio was also higher in the operation group (0.541±0.126 vs. 0.278±0.049, p=0.002). Upon autoradiography, 64Cu-ATSM uptake was observed in the fundus in the operation group, and was well-correlated to that observed on the PET image. Upon immunohistochemistry, expression of hypoxia-inducible factor 1a and pimonidazole were significantly increased at the fundus and lesser curvature compared to the greater curvature in the operation group.

Conclusion: Hypoxia PET imaging with 64Cu-ATSM can detect ischemia in a rat esophagectomy model. Further clinical studies are needed to verify whether hypoxia imaging may be useful in humans.

No MeSH data available.


Related in: MedlinePlus

MicroPET imaging.A. Operation group. 64Cu-ATSM uptake was observed in the fundus (dotted line). B. Control group. 64Cu-ATSM uptake was not observed in the fundus. C. Comparison of %ID/g values between the operation and control groups. D. Comparison of the fundus/liver ratio (%ID/g of fundus area by %ID/g of liver) between the operation and control groups.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476727&req=5

pone.0131083.g002: MicroPET imaging.A. Operation group. 64Cu-ATSM uptake was observed in the fundus (dotted line). B. Control group. 64Cu-ATSM uptake was not observed in the fundus. C. Comparison of %ID/g values between the operation and control groups. D. Comparison of the fundus/liver ratio (%ID/g of fundus area by %ID/g of liver) between the operation and control groups.

Mentions: On static PET imaging, Cu-ATSM uptake at the fundus was observed in the operation group on the 3-h PET image (Fig 2A), but such abnormal 64Cu-ATSM uptake was not evident in the control group (Fig 2B). The mean %ID/g of the fundus in the operation group was 0.047 ± 0.015. In the control group, the area corresponding to the fundus was chosen as an ROI, because there was no definite area of uptake in the control group, and the mean %ID/g of this area was 0.026 ± 0.006; the difference was significant (Mann-Whitney test, p = 0.021, Fig 2C). The fundus/liver ratios were 0.541 ± 0.126 and 0.278 ± 0.049, respectively (operation group and control group, Mann-Whitney test, p = 0.002, Fig 2D).


64Cu-ATSM Hypoxia Positron Emission Tomography for Detection of Conduit Ischemia in an Experimental Rat Esophagectomy Model.

Park SY, Kang WJ, Cho A, Chae JR, Cho YL, Kim JY, Lee JW, Chung KY - PLoS ONE (2015)

MicroPET imaging.A. Operation group. 64Cu-ATSM uptake was observed in the fundus (dotted line). B. Control group. 64Cu-ATSM uptake was not observed in the fundus. C. Comparison of %ID/g values between the operation and control groups. D. Comparison of the fundus/liver ratio (%ID/g of fundus area by %ID/g of liver) between the operation and control groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476727&req=5

pone.0131083.g002: MicroPET imaging.A. Operation group. 64Cu-ATSM uptake was observed in the fundus (dotted line). B. Control group. 64Cu-ATSM uptake was not observed in the fundus. C. Comparison of %ID/g values between the operation and control groups. D. Comparison of the fundus/liver ratio (%ID/g of fundus area by %ID/g of liver) between the operation and control groups.
Mentions: On static PET imaging, Cu-ATSM uptake at the fundus was observed in the operation group on the 3-h PET image (Fig 2A), but such abnormal 64Cu-ATSM uptake was not evident in the control group (Fig 2B). The mean %ID/g of the fundus in the operation group was 0.047 ± 0.015. In the control group, the area corresponding to the fundus was chosen as an ROI, because there was no definite area of uptake in the control group, and the mean %ID/g of this area was 0.026 ± 0.006; the difference was significant (Mann-Whitney test, p = 0.021, Fig 2C). The fundus/liver ratios were 0.541 ± 0.126 and 0.278 ± 0.049, respectively (operation group and control group, Mann-Whitney test, p = 0.002, Fig 2D).

Bottom Line: Upon autoradiography, 64Cu-ATSM uptake was observed in the fundus in the operation group, and was well-correlated to that observed on the PET image.Upon immunohistochemistry, expression of hypoxia-inducible factor 1a and pimonidazole were significantly increased at the fundus and lesser curvature compared to the greater curvature in the operation group.Further clinical studies are needed to verify whether hypoxia imaging may be useful in humans.

View Article: PubMed Central - PubMed

Affiliation: Department of Thoracic and Cardiovascular Surgery, Yonsei University, College of Medicine, Seoul, Republic of Korea; Department of Thoracic and Cardiovascular Surgery, Ajou University, School of Medicine, Suwon, Republic of Korea.

ABSTRACT

Background: We designed a hypoxia-imaging modality to detect ischemia of the gastric conduit after esophagectomy.

Materials and methods: A rat esophagectomy model was created using 12-16-week-old, 300-350 g male Sprague-Dawley rats. In the operation group (n=6), partial gastric devascularization was performed by ligating the left gastric artery and the short gastric arteries and an esophagogastric anastomosis was performed. In the control group (n=6), the esophageal-gastric junction was incised and suturing was performed without gastric devascularization. Positron emission tomography (PET) images were taken using a microPET rodent model scanner, 24 h after the initial operation, after injection of 200 μCi 64Cu-diacetyl-bis (N4-methylsemicarbazone) (64Cu-ATSM) and pimonidazole 120 mg/kg. After microPET imaging, autoradiography and immunohistochemistry were performed.

Results: The PET image revealed 64Cu-ATSM uptake at the fundus in the operation group 3 h after 64Cu-ATSM injection. The maximum percentage of the injected dose per gram of tissue was higher in the operation group (0.047±0.015 vs. 0.026±0.006, p=0.021). The fundus/liver ratio was also higher in the operation group (0.541±0.126 vs. 0.278±0.049, p=0.002). Upon autoradiography, 64Cu-ATSM uptake was observed in the fundus in the operation group, and was well-correlated to that observed on the PET image. Upon immunohistochemistry, expression of hypoxia-inducible factor 1a and pimonidazole were significantly increased at the fundus and lesser curvature compared to the greater curvature in the operation group.

Conclusion: Hypoxia PET imaging with 64Cu-ATSM can detect ischemia in a rat esophagectomy model. Further clinical studies are needed to verify whether hypoxia imaging may be useful in humans.

No MeSH data available.


Related in: MedlinePlus