Limits...
Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus

Size descriptors plot of the piamater, white matter, amygdala, hippocampus and temporal horn of the lateral ventricle by age group (LT and GT), in both MRI and histological studies, and for both right and left hemispheres.Data are mean and standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476703&req=5

pone.0130314.g004: Size descriptors plot of the piamater, white matter, amygdala, hippocampus and temporal horn of the lateral ventricle by age group (LT and GT), in both MRI and histological studies, and for both right and left hemispheres.Data are mean and standard deviation.

Mentions: The morphometric results obtained for piamater, white matter boundary and profiles of the amygdala, HF and lateral ventricle are shown in Figs 3 and 4 (size and shape descriptors, respectively). None of the descriptors showed statistical differences between hemispheres for both MRI and histological studies (mean and SD values are shown in Figs 3 and 4).


Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

Size descriptors plot of the piamater, white matter, amygdala, hippocampus and temporal horn of the lateral ventricle by age group (LT and GT), in both MRI and histological studies, and for both right and left hemispheres.Data are mean and standard deviation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476703&req=5

pone.0130314.g004: Size descriptors plot of the piamater, white matter, amygdala, hippocampus and temporal horn of the lateral ventricle by age group (LT and GT), in both MRI and histological studies, and for both right and left hemispheres.Data are mean and standard deviation.
Mentions: The morphometric results obtained for piamater, white matter boundary and profiles of the amygdala, HF and lateral ventricle are shown in Figs 3 and 4 (size and shape descriptors, respectively). None of the descriptors showed statistical differences between hemispheres for both MRI and histological studies (mean and SD values are shown in Figs 3 and 4).

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus