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Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus

(a) MRI of the temporal lobe. A test system of regularly spaced points is superimposed for the estimation of volume of different structures. The number of points that hit the structure result in the volume, according to the formulae presented in Methods. The area associated to test point is showed (a/p). (b) Histological section stained with thionin at a roughly the same level as in a MRI image, where horizontal lines were superimposed to estimate the intercepts between test lines and each structure of interest (outline of the white matter, grey matter-surface area of the temporal lobe-, profiles of the amygdala, hippocampus and lateral ventricle). The length of test line associated with a test point is also represented.
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pone.0130314.g002: (a) MRI of the temporal lobe. A test system of regularly spaced points is superimposed for the estimation of volume of different structures. The number of points that hit the structure result in the volume, according to the formulae presented in Methods. The area associated to test point is showed (a/p). (b) Histological section stained with thionin at a roughly the same level as in a MRI image, where horizontal lines were superimposed to estimate the intercepts between test lines and each structure of interest (outline of the white matter, grey matter-surface area of the temporal lobe-, profiles of the amygdala, hippocampus and lateral ventricle). The length of test line associated with a test point is also represented.

Mentions: The stereological study included the estimation of the surface area and volume of the structures analyzed (Fig 2). The volume of both the grey and white matter, amygdaloid complex, HF, as well as the temporal horn of the lateral ventricle was estimated using Cavalieri’s principle [19]. This method allows an efficient and precise volume estimation of a structure regardless of its size and shape, through a series of parallel planes spaced at a constant distance “t”. The final estimation of the volume is obtained according to the following equation:est(V) = t•(A1+ A2+ A3+…An)in which, A1, A2, … An denote the outline sectional area of each structure, and t, is the section interval for the n consecutive sections. Also, we superimposed an overlay made up of test points regularly spaced for the volume estimation; thus, the sum of points ΣP hitting the structure of interest, multiplied by the section interval, t, and the area associated with each test point a/p, is a useful estimation of the volume. This estimation is expressed as:est(V) = t•(a/p)•ΣP


Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

(a) MRI of the temporal lobe. A test system of regularly spaced points is superimposed for the estimation of volume of different structures. The number of points that hit the structure result in the volume, according to the formulae presented in Methods. The area associated to test point is showed (a/p). (b) Histological section stained with thionin at a roughly the same level as in a MRI image, where horizontal lines were superimposed to estimate the intercepts between test lines and each structure of interest (outline of the white matter, grey matter-surface area of the temporal lobe-, profiles of the amygdala, hippocampus and lateral ventricle). The length of test line associated with a test point is also represented.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476703&req=5

pone.0130314.g002: (a) MRI of the temporal lobe. A test system of regularly spaced points is superimposed for the estimation of volume of different structures. The number of points that hit the structure result in the volume, according to the formulae presented in Methods. The area associated to test point is showed (a/p). (b) Histological section stained with thionin at a roughly the same level as in a MRI image, where horizontal lines were superimposed to estimate the intercepts between test lines and each structure of interest (outline of the white matter, grey matter-surface area of the temporal lobe-, profiles of the amygdala, hippocampus and lateral ventricle). The length of test line associated with a test point is also represented.
Mentions: The stereological study included the estimation of the surface area and volume of the structures analyzed (Fig 2). The volume of both the grey and white matter, amygdaloid complex, HF, as well as the temporal horn of the lateral ventricle was estimated using Cavalieri’s principle [19]. This method allows an efficient and precise volume estimation of a structure regardless of its size and shape, through a series of parallel planes spaced at a constant distance “t”. The final estimation of the volume is obtained according to the following equation:est(V) = t•(A1+ A2+ A3+…An)in which, A1, A2, … An denote the outline sectional area of each structure, and t, is the section interval for the n consecutive sections. Also, we superimposed an overlay made up of test points regularly spaced for the volume estimation; thus, the sum of points ΣP hitting the structure of interest, multiplied by the section interval, t, and the area associated with each test point a/p, is a useful estimation of the volume. This estimation is expressed as:est(V) = t•(a/p)•ΣP

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus