Limits...
Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus

(a) Representation of an hemisphere showing the intercommissural line (A-B line) and the perpendicular sections to obtain consecutive slabs with a thickness of t. (b) A MRI at the level of the amygdala, caudal to the limen insulae. (c) MRI depicting the outline the temporal lobe structures. (d) Histological delineation of structures at same level as in panel c. WM: White matter; GM: Grey matter; A: Amygdala; Hp: Hippocampus; LV: Temporal horn of the lateral ventricle; ThEC: scheme with the five measurements of the entorhinal cortex; ThTL: similar representation of the five measurements in the temporal cortex.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476703&req=5

pone.0130314.g001: (a) Representation of an hemisphere showing the intercommissural line (A-B line) and the perpendicular sections to obtain consecutive slabs with a thickness of t. (b) A MRI at the level of the amygdala, caudal to the limen insulae. (c) MRI depicting the outline the temporal lobe structures. (d) Histological delineation of structures at same level as in panel c. WM: White matter; GM: Grey matter; A: Amygdala; Hp: Hippocampus; LV: Temporal horn of the lateral ventricle; ThEC: scheme with the five measurements of the entorhinal cortex; ThTL: similar representation of the five measurements in the temporal cortex.

Mentions: The brains were cut in several slabs of approximately 1 cm-thick in the coronal plane, orthogonal to the intercommissural line (according to the standard procedure of [15] (Fig 1); samples were obtained for neuropahological examination. Brain slabs were fixed by immersion in 10% buffered formalin, and subsequently in 4% paraformaldehyde during at least eight weeks. Thereafter, the brain slabs were hold together in a gauze for MRI study using 1.5T magnetic resonance scanner (Gyroscan Intera, Phillips); a gradient SE coronal T1 high resolution sequence was used once brains were placed on the head matrix coil; consecutive MRI sections of the brain were carried out with a thickness of 1.2 mm and an acquisition matrix of 512x512; the effective pixel size was of 0.29 mm.


Quantitative Measurements in the Human Hippocampus and Related Areas: Correspondence between Ex-Vivo MRI and Histological Preparations.

Delgado-González JC, Mansilla-Legorburo F, Florensa-Vila J, Insausti AM, Viñuela A, Tuñón-Alvarez T, Cruz M, Mohedano-Moriano A, Insausti R, Artacho-Pérula E - PLoS ONE (2015)

(a) Representation of an hemisphere showing the intercommissural line (A-B line) and the perpendicular sections to obtain consecutive slabs with a thickness of t. (b) A MRI at the level of the amygdala, caudal to the limen insulae. (c) MRI depicting the outline the temporal lobe structures. (d) Histological delineation of structures at same level as in panel c. WM: White matter; GM: Grey matter; A: Amygdala; Hp: Hippocampus; LV: Temporal horn of the lateral ventricle; ThEC: scheme with the five measurements of the entorhinal cortex; ThTL: similar representation of the five measurements in the temporal cortex.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476703&req=5

pone.0130314.g001: (a) Representation of an hemisphere showing the intercommissural line (A-B line) and the perpendicular sections to obtain consecutive slabs with a thickness of t. (b) A MRI at the level of the amygdala, caudal to the limen insulae. (c) MRI depicting the outline the temporal lobe structures. (d) Histological delineation of structures at same level as in panel c. WM: White matter; GM: Grey matter; A: Amygdala; Hp: Hippocampus; LV: Temporal horn of the lateral ventricle; ThEC: scheme with the five measurements of the entorhinal cortex; ThTL: similar representation of the five measurements in the temporal cortex.
Mentions: The brains were cut in several slabs of approximately 1 cm-thick in the coronal plane, orthogonal to the intercommissural line (according to the standard procedure of [15] (Fig 1); samples were obtained for neuropahological examination. Brain slabs were fixed by immersion in 10% buffered formalin, and subsequently in 4% paraformaldehyde during at least eight weeks. Thereafter, the brain slabs were hold together in a gauze for MRI study using 1.5T magnetic resonance scanner (Gyroscan Intera, Phillips); a gradient SE coronal T1 high resolution sequence was used once brains were placed on the head matrix coil; consecutive MRI sections of the brain were carried out with a thickness of 1.2 mm and an acquisition matrix of 512x512; the effective pixel size was of 0.29 mm.

Bottom Line: Only values in perimeter and circularity of the piamater were different.This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement.Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect.

View Article: PubMed Central - PubMed

Affiliation: Human Neuroanatomy Laboratory and C.R.I.B., School of Medicine, University of Castilla-La Mancha, Albacete, Spain.

ABSTRACT
The decrease of volume estimates in different structures of the medial temporal lobe related to memory correlate with the decline of cognitive functions in neurodegenerative diseases. This study presents data on the association between MRI quantitative parameters of medial temporal lobe structures and their quantitative estimate in microscopic examination. Twelve control cases had ex-vivo MRI, and thereafter, the temporal lobe of both hemispheres was sectioned from the pole as far as the level of the splenium of the corpus callosum. Nissl stain was used to establish anatomical boundaries between structures in the medial temporal lobe. The study included morphometrical and stereological estimates of the amygdaloid complex, hippocampus, and temporal horn of the lateral ventricle, as well as different regions of grey and white matter in the temporal lobe. Data showed a close association between morphometric MRI images values and those based on the histological determination of boundaries. Only values in perimeter and circularity of the piamater were different. This correspondence is also revealed by the stereological study, although irregular compartments resulted in a lesser agreement. Neither age (< 65 yr and > 65 yr) nor hemisphere had any effect. Our results indicate that ex-vivo MRI is highly associated with quantitative information gathered by histological examination, and these data could be used as structural MRI biomarker in neurodegenerative diseases.

No MeSH data available.


Related in: MedlinePlus