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A Difference in Fatty Acid Composition of Isocaloric High-Fat Diets Alters Metabolic Flexibility in Male C57BL/6JOlaHsd Mice.

Duivenvoorde LP, van Schothorst EM, Swarts HM, Kuda O, Steenbergh E, Termeulen S, Kopecky J, Keijer J - PLoS ONE (2015)

Bottom Line: We found that the HFs diet, compared to the HFpu diet, increased mean adipocyte size, liver damage, and ectopic lipid storage in liver and muscle; although, we did not find differences in body weight, total adiposity, adipose tissue health, serum adipokines, whole body energy balance, or circadian rhythm between HFs and HFpu mice.To conclude, the HFs versus the HFpu diet increased ectopic fat storage, liver damage, and mean adipocyte size and reduced metabolic flexibility in male mice.This study underscores the physiological relevance of indirect calorimetry-based challenge tests.

View Article: PubMed Central - PubMed

Affiliation: Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
Poly-unsaturated fatty acids (PUFAs) are considered to be healthier than saturated fatty acids (SFAs), but others postulate that especially the ratio of omega-6 to omega-3 PUFAs (n6/n3 ratio) determines health. Health can be determined with biomarkers, but functional health status is likely better reflected by challenge tests that assess metabolic flexibility. The aim of this study was to determine the effect of high-fat diets with different fatty acid compositions, but similar n6/n3 ratio, on metabolic flexibility. Therefore, adult male mice received isocaloric high-fat diets with either predominantly PUFAs (HFpu diet) or predominantly SFAs (HFs diet) but similar n6/n3 ratio for six months, during and after which several biomarkers for health were measured. Metabolic flexibility was assessed by the response to an oral glucose tolerance test, a fasting and re-feeding test and an oxygen restriction test (OxR; normobaric hypoxia). The latter two are non-invasive, indirect calorimetry-based tests that measure the adaptive capacity of the body as a whole. We found that the HFs diet, compared to the HFpu diet, increased mean adipocyte size, liver damage, and ectopic lipid storage in liver and muscle; although, we did not find differences in body weight, total adiposity, adipose tissue health, serum adipokines, whole body energy balance, or circadian rhythm between HFs and HFpu mice. HFs mice were, furthermore, less flexible in their response to both fasting- re-feeding and OxR, while glucose tolerance was indistinguishable. To conclude, the HFs versus the HFpu diet increased ectopic fat storage, liver damage, and mean adipocyte size and reduced metabolic flexibility in male mice. This study underscores the physiological relevance of indirect calorimetry-based challenge tests.

No MeSH data available.


Related in: MedlinePlus

Body weight and total adiposity during 27 weeks of HF feeding.Body weight (solid lines) and total adiposity (dotted lines) were determined on a weekly basis during the 27 weeks of HFpu and HFs feeding. Total adiposity is expressed as the percentage of total body fat over body weight.
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pone.0128515.g001: Body weight and total adiposity during 27 weeks of HF feeding.Body weight (solid lines) and total adiposity (dotted lines) were determined on a weekly basis during the 27 weeks of HFpu and HFs feeding. Total adiposity is expressed as the percentage of total body fat over body weight.

Mentions: After 27 weeks of HF feeding, we detected no differences in BW, adiposity (Fig 1), cumulative feed intake, WAT mitochondrial density, serum glucose, insulin, leptin, or adiponectin levels (Table 2) between HFpu and HFs mice. Of note, both groups of mice had relatively high fasting insulin levels that are well above levels that are usually seen in high-fat fed male C57BL/6J mice (around 0.11 nM after 18 weeks of feeding [44]).


A Difference in Fatty Acid Composition of Isocaloric High-Fat Diets Alters Metabolic Flexibility in Male C57BL/6JOlaHsd Mice.

Duivenvoorde LP, van Schothorst EM, Swarts HM, Kuda O, Steenbergh E, Termeulen S, Kopecky J, Keijer J - PLoS ONE (2015)

Body weight and total adiposity during 27 weeks of HF feeding.Body weight (solid lines) and total adiposity (dotted lines) were determined on a weekly basis during the 27 weeks of HFpu and HFs feeding. Total adiposity is expressed as the percentage of total body fat over body weight.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476692&req=5

pone.0128515.g001: Body weight and total adiposity during 27 weeks of HF feeding.Body weight (solid lines) and total adiposity (dotted lines) were determined on a weekly basis during the 27 weeks of HFpu and HFs feeding. Total adiposity is expressed as the percentage of total body fat over body weight.
Mentions: After 27 weeks of HF feeding, we detected no differences in BW, adiposity (Fig 1), cumulative feed intake, WAT mitochondrial density, serum glucose, insulin, leptin, or adiponectin levels (Table 2) between HFpu and HFs mice. Of note, both groups of mice had relatively high fasting insulin levels that are well above levels that are usually seen in high-fat fed male C57BL/6J mice (around 0.11 nM after 18 weeks of feeding [44]).

Bottom Line: We found that the HFs diet, compared to the HFpu diet, increased mean adipocyte size, liver damage, and ectopic lipid storage in liver and muscle; although, we did not find differences in body weight, total adiposity, adipose tissue health, serum adipokines, whole body energy balance, or circadian rhythm between HFs and HFpu mice.To conclude, the HFs versus the HFpu diet increased ectopic fat storage, liver damage, and mean adipocyte size and reduced metabolic flexibility in male mice.This study underscores the physiological relevance of indirect calorimetry-based challenge tests.

View Article: PubMed Central - PubMed

Affiliation: Human and Animal Physiology, Wageningen University, Wageningen, The Netherlands.

ABSTRACT
Poly-unsaturated fatty acids (PUFAs) are considered to be healthier than saturated fatty acids (SFAs), but others postulate that especially the ratio of omega-6 to omega-3 PUFAs (n6/n3 ratio) determines health. Health can be determined with biomarkers, but functional health status is likely better reflected by challenge tests that assess metabolic flexibility. The aim of this study was to determine the effect of high-fat diets with different fatty acid compositions, but similar n6/n3 ratio, on metabolic flexibility. Therefore, adult male mice received isocaloric high-fat diets with either predominantly PUFAs (HFpu diet) or predominantly SFAs (HFs diet) but similar n6/n3 ratio for six months, during and after which several biomarkers for health were measured. Metabolic flexibility was assessed by the response to an oral glucose tolerance test, a fasting and re-feeding test and an oxygen restriction test (OxR; normobaric hypoxia). The latter two are non-invasive, indirect calorimetry-based tests that measure the adaptive capacity of the body as a whole. We found that the HFs diet, compared to the HFpu diet, increased mean adipocyte size, liver damage, and ectopic lipid storage in liver and muscle; although, we did not find differences in body weight, total adiposity, adipose tissue health, serum adipokines, whole body energy balance, or circadian rhythm between HFs and HFpu mice. HFs mice were, furthermore, less flexible in their response to both fasting- re-feeding and OxR, while glucose tolerance was indistinguishable. To conclude, the HFs versus the HFpu diet increased ectopic fat storage, liver damage, and mean adipocyte size and reduced metabolic flexibility in male mice. This study underscores the physiological relevance of indirect calorimetry-based challenge tests.

No MeSH data available.


Related in: MedlinePlus