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Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow.

López-Úbeda R, García-Vázquez FA, Romar R, Gadea J, Muñoz M, Hunter RH, Coy P - PLoS ONE (2015)

Bottom Line: Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3).Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract.These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Murcia, Spain; International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain.

ABSTRACT
Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were in natural oestrus) and no artificial sperm selection (selection was performed within the female genital) were imposed. It is therefore emphasised that no surgical introduction of spermatozoa and no insemination at a site other than the physiological one were used. This approach revealed 17 genes that were two-fold or more up-regulated in oviducts exposed to spermatozoa and/or developing embryos and 9 genes that were two-fold or more down-regulated. Functional analysis of the genes revealed that the top canonical pathways affected by insemination were related to the inflammatory response and immune system (Network 1) to molecular transport, protein trafficking and developmental disorder (Network 2) and to cell-to-cell signalling and interaction (Network 3). Some of the genes in network 1 had been previously detected in the oviduct of human and animals, where they were over-expressed in the presence of spermatozoa or pre-implantation embryos (C3, IGHG1, ITIH4, TNF and SERPINE1) whereas others were not previously reported (SAA2, ALOX12, CD1D and SPP1). Genes in Network 2 included RAB1B and TOR3A, the latter being described for the first time in the oviduct and clearly expressed in the epithelial cells of the mucosa layer. Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3). Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract. These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development.

No MeSH data available.


Related in: MedlinePlus

Interactome of functional associations among genes included in Network 2 by Ingenuity Pathway Analysis.
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pone.0130128.g004: Interactome of functional associations among genes included in Network 2 by Ingenuity Pathway Analysis.

Mentions: The top canonical pathway associated with the DEGs clustered in Network 2 (Fig 4), are related to molecular transport, protein trafficking and developmental disorder. TOR3A and RAB1B, the two genes with the highest positive fold change of this study were found in this network. RAB1B is required for clearance of ubiquitinated protein aggregates [49], and the boar sperm acrosome shows a high level of de-ubiquitinating activity (ubiquitin C hydrolases 1 and 3, UCHL1/L3) related to regulation of penetration through the zona pellucida [50]. Differences in the expression of RAB1B could be related to the presumed increase of ubiquitinated protein aggregates prompted by the presence of spermatozoa in the oviduct.


Oviductal Transcriptome Is Modified after Insemination during Spontaneous Ovulation in the Sow.

López-Úbeda R, García-Vázquez FA, Romar R, Gadea J, Muñoz M, Hunter RH, Coy P - PLoS ONE (2015)

Interactome of functional associations among genes included in Network 2 by Ingenuity Pathway Analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476686&req=5

pone.0130128.g004: Interactome of functional associations among genes included in Network 2 by Ingenuity Pathway Analysis.
Mentions: The top canonical pathway associated with the DEGs clustered in Network 2 (Fig 4), are related to molecular transport, protein trafficking and developmental disorder. TOR3A and RAB1B, the two genes with the highest positive fold change of this study were found in this network. RAB1B is required for clearance of ubiquitinated protein aggregates [49], and the boar sperm acrosome shows a high level of de-ubiquitinating activity (ubiquitin C hydrolases 1 and 3, UCHL1/L3) related to regulation of penetration through the zona pellucida [50]. Differences in the expression of RAB1B could be related to the presumed increase of ubiquitinated protein aggregates prompted by the presence of spermatozoa in the oviduct.

Bottom Line: Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3).Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract.These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development.

View Article: PubMed Central - PubMed

Affiliation: Department of Physiology, Veterinary Faculty, University of Murcia, Murcia, Spain; International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), Murcia, Spain.

ABSTRACT
Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were in natural oestrus) and no artificial sperm selection (selection was performed within the female genital) were imposed. It is therefore emphasised that no surgical introduction of spermatozoa and no insemination at a site other than the physiological one were used. This approach revealed 17 genes that were two-fold or more up-regulated in oviducts exposed to spermatozoa and/or developing embryos and 9 genes that were two-fold or more down-regulated. Functional analysis of the genes revealed that the top canonical pathways affected by insemination were related to the inflammatory response and immune system (Network 1) to molecular transport, protein trafficking and developmental disorder (Network 2) and to cell-to-cell signalling and interaction (Network 3). Some of the genes in network 1 had been previously detected in the oviduct of human and animals, where they were over-expressed in the presence of spermatozoa or pre-implantation embryos (C3, IGHG1, ITIH4, TNF and SERPINE1) whereas others were not previously reported (SAA2, ALOX12, CD1D and SPP1). Genes in Network 2 included RAB1B and TOR3A, the latter being described for the first time in the oviduct and clearly expressed in the epithelial cells of the mucosa layer. Network 3 integrated the genes with the highest down-regulation level (CYP51, PTH1R and TMOD3). Data in the present study indicate a change in gene expression during gamete encounter at the site of fertilization after a natural sperm selection within the female genital tract. These changes would indicate a modification of the environment preparing the oviduct for a successful fertilization and for an adequate embryo early development.

No MeSH data available.


Related in: MedlinePlus