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Terminal Restriction Fragment Length Polymorphism Analysis of Soil Bacterial Communities under Different Vegetation Types in Subtropical Area.

Wu Z, Lin W, Li B, Wu L, Fang C, Zhang Z - PLoS ONE (2015)

Bottom Line: The order of Shannon-Wiener index (H) of all soil samples was in the order of EBF>CF>SDF>AM, whereas bacterial species richness as estimated by four restriction enzymes indicated no significant difference.Principal component analysis (PCA) revealed that the soil bacterial communities' structures of EBF, CF, SDF and AM were clearly separated along the first and second principal components, which explained 62.17% and 31.58% of the total variance, respectively.The soil physical-chemical properties such as total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) and total potassium (TK) were positively correlated with the diversity of bacterial communities.

View Article: PubMed Central - PubMed

Affiliation: Life Sciences College of Fujian Agriculture and Forestry University, Fujian, China.

ABSTRACT
Soil microbes are active players in energy flow and material exchange of the forest ecosystems, but the research on the relationship between the microbial diversity and the vegetation types is less conducted, especially in the subtropical area of China. In this present study, the rhizosphere soils of evergreen broad-leaf forest (EBF), coniferous forest (CF), subalpine dwarf forest (SDF) and alpine meadow (AM) were chosen as test sites. Terminal-restriction fragment length polymorphisms (T-RFLP) analysis was used to detect the composition and diversity of soil bacterial communities under different vegetation types in the National Natural Reserve of Wuyi Mountains. Our results revealed distinct differences in soil microbial composition under different vegetation types. Total 73 microbes were identified in soil samples of the four vegetation types, and 56, 49, 46 and 36 clones were obtained from the soils of EBF, CF, SDF and AM, respectively, and subsequently sequenced. The Actinobacteria, Fusobacterium, Bacteroidetes and Proteobacteria were the most predominant in all soil samples. The order of Shannon-Wiener index (H) of all soil samples was in the order of EBF>CF>SDF>AM, whereas bacterial species richness as estimated by four restriction enzymes indicated no significant difference. Principal component analysis (PCA) revealed that the soil bacterial communities' structures of EBF, CF, SDF and AM were clearly separated along the first and second principal components, which explained 62.17% and 31.58% of the total variance, respectively. The soil physical-chemical properties such as total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) and total potassium (TK) were positively correlated with the diversity of bacterial communities.

No MeSH data available.


Related in: MedlinePlus

Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient.The figure describes the variance of bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other.
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pone.0129397.g002: Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient.The figure describes the variance of bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other.

Mentions: Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient was showed in Fig 2 (Table E in S1 File). The PCA score plot of T-RF data revealed that the structures of soil bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other, with EBF and SDF on the left side, and CF and AM on the right side of the axis, which described 62.17% and 31.58% of the total variance, respectively.


Terminal Restriction Fragment Length Polymorphism Analysis of Soil Bacterial Communities under Different Vegetation Types in Subtropical Area.

Wu Z, Lin W, Li B, Wu L, Fang C, Zhang Z - PLoS ONE (2015)

Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient.The figure describes the variance of bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476674&req=5

pone.0129397.g002: Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient.The figure describes the variance of bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other.
Mentions: Principal components analysis (PCA) of T-RFLP data in different vegetation types along an altitude gradient was showed in Fig 2 (Table E in S1 File). The PCA score plot of T-RF data revealed that the structures of soil bacterial communities in the EBF, CF, SDF and AM sites were clearly different from each other, with EBF and SDF on the left side, and CF and AM on the right side of the axis, which described 62.17% and 31.58% of the total variance, respectively.

Bottom Line: The order of Shannon-Wiener index (H) of all soil samples was in the order of EBF>CF>SDF>AM, whereas bacterial species richness as estimated by four restriction enzymes indicated no significant difference.Principal component analysis (PCA) revealed that the soil bacterial communities' structures of EBF, CF, SDF and AM were clearly separated along the first and second principal components, which explained 62.17% and 31.58% of the total variance, respectively.The soil physical-chemical properties such as total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) and total potassium (TK) were positively correlated with the diversity of bacterial communities.

View Article: PubMed Central - PubMed

Affiliation: Life Sciences College of Fujian Agriculture and Forestry University, Fujian, China.

ABSTRACT
Soil microbes are active players in energy flow and material exchange of the forest ecosystems, but the research on the relationship between the microbial diversity and the vegetation types is less conducted, especially in the subtropical area of China. In this present study, the rhizosphere soils of evergreen broad-leaf forest (EBF), coniferous forest (CF), subalpine dwarf forest (SDF) and alpine meadow (AM) were chosen as test sites. Terminal-restriction fragment length polymorphisms (T-RFLP) analysis was used to detect the composition and diversity of soil bacterial communities under different vegetation types in the National Natural Reserve of Wuyi Mountains. Our results revealed distinct differences in soil microbial composition under different vegetation types. Total 73 microbes were identified in soil samples of the four vegetation types, and 56, 49, 46 and 36 clones were obtained from the soils of EBF, CF, SDF and AM, respectively, and subsequently sequenced. The Actinobacteria, Fusobacterium, Bacteroidetes and Proteobacteria were the most predominant in all soil samples. The order of Shannon-Wiener index (H) of all soil samples was in the order of EBF>CF>SDF>AM, whereas bacterial species richness as estimated by four restriction enzymes indicated no significant difference. Principal component analysis (PCA) revealed that the soil bacterial communities' structures of EBF, CF, SDF and AM were clearly separated along the first and second principal components, which explained 62.17% and 31.58% of the total variance, respectively. The soil physical-chemical properties such as total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) and total potassium (TK) were positively correlated with the diversity of bacterial communities.

No MeSH data available.


Related in: MedlinePlus