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Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus

Luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) reduced mRNA expression of MMP-2 and MMP-9 in ES-2 cells.(a) LU3′O-GP effects on MMP-2 expression; (b) LU3′O-GP effects on MMP-9 expression. mRNA levels were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), using glyceraldehyde 3-phosphate dehydrogenase as the loading control. RT-PCR products were detected by ethidium bromide staining. Values represent the means of triplicate experiments. *p < 0.05, as compared to the control.
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pone.0130685.g008: Luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) reduced mRNA expression of MMP-2 and MMP-9 in ES-2 cells.(a) LU3′O-GP effects on MMP-2 expression; (b) LU3′O-GP effects on MMP-9 expression. mRNA levels were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), using glyceraldehyde 3-phosphate dehydrogenase as the loading control. RT-PCR products were detected by ethidium bromide staining. Values represent the means of triplicate experiments. *p < 0.05, as compared to the control.

Mentions: To further determine the influence of LU3′O-GP on the upstream factors that are important for the regulation of tumor cell invasion, the mRNA and protein expression of MMP2 and MMP9 were investigated. The RT-PCR assay results are shown in Fig 8. The levels of MMP2 and MMP9 mRNAs were reduced in a concentration-dependent manner by treatment with LU3′O-GP, Western blotting analysis (Fig 9) showed a similar suppression of MMP-2 (pro-MMP2 and activity MMP-2) and MMP-9 protein expression by LU3′O-GP. These results suggested that the flavonoid, LU3′O-GP, suppressed expression of MMP2 and MMP9 at both the mRNA and protein levels.


Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) reduced mRNA expression of MMP-2 and MMP-9 in ES-2 cells.(a) LU3′O-GP effects on MMP-2 expression; (b) LU3′O-GP effects on MMP-9 expression. mRNA levels were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), using glyceraldehyde 3-phosphate dehydrogenase as the loading control. RT-PCR products were detected by ethidium bromide staining. Values represent the means of triplicate experiments. *p < 0.05, as compared to the control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476667&req=5

pone.0130685.g008: Luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) reduced mRNA expression of MMP-2 and MMP-9 in ES-2 cells.(a) LU3′O-GP effects on MMP-2 expression; (b) LU3′O-GP effects on MMP-9 expression. mRNA levels were investigated by reverse transcriptase polymerase chain reaction (RT-PCR), using glyceraldehyde 3-phosphate dehydrogenase as the loading control. RT-PCR products were detected by ethidium bromide staining. Values represent the means of triplicate experiments. *p < 0.05, as compared to the control.
Mentions: To further determine the influence of LU3′O-GP on the upstream factors that are important for the regulation of tumor cell invasion, the mRNA and protein expression of MMP2 and MMP9 were investigated. The RT-PCR assay results are shown in Fig 8. The levels of MMP2 and MMP9 mRNAs were reduced in a concentration-dependent manner by treatment with LU3′O-GP, Western blotting analysis (Fig 9) showed a similar suppression of MMP-2 (pro-MMP2 and activity MMP-2) and MMP-9 protein expression by LU3′O-GP. These results suggested that the flavonoid, LU3′O-GP, suppressed expression of MMP2 and MMP9 at both the mRNA and protein levels.

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus