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Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus

Effects of P. crispus flavonoids on ES-2 cell invasion.Cells were treated with luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) or flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their invasion was quantified in a Transwell chamber membrane with Matrigel. Values represent the means of experiments performed in triplicate. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
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pone.0130685.g007: Effects of P. crispus flavonoids on ES-2 cell invasion.Cells were treated with luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) or flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their invasion was quantified in a Transwell chamber membrane with Matrigel. Values represent the means of experiments performed in triplicate. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.

Mentions: During tumor metastasis, the cells must pass through the ECM. To evaluate whether LU3′O-GP affected ES-2 cell invasion, cell invasion assays were carried out in a Transwell chamber coated with Matrigel. Treatment of ES-2 cells with increasing concentrations of LU3′O-GP led to a significant concentration-dependent decrease in the cell invasion rate, as compared with the control (Fig 7). This process was inhibited by about 73.7% and 89.9% in the presence of 30 and 60 μg/mL LU3′O-GP, respectively, while the inhibition induced by 100 μg/mL FL6C-GP was only 6.7%. The results of this assay demonstrated that LU3′O-GP dramatically inhibited the invasion of ES-2 cells.


Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Effects of P. crispus flavonoids on ES-2 cell invasion.Cells were treated with luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) or flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their invasion was quantified in a Transwell chamber membrane with Matrigel. Values represent the means of experiments performed in triplicate. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476667&req=5

pone.0130685.g007: Effects of P. crispus flavonoids on ES-2 cell invasion.Cells were treated with luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) or flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their invasion was quantified in a Transwell chamber membrane with Matrigel. Values represent the means of experiments performed in triplicate. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
Mentions: During tumor metastasis, the cells must pass through the ECM. To evaluate whether LU3′O-GP affected ES-2 cell invasion, cell invasion assays were carried out in a Transwell chamber coated with Matrigel. Treatment of ES-2 cells with increasing concentrations of LU3′O-GP led to a significant concentration-dependent decrease in the cell invasion rate, as compared with the control (Fig 7). This process was inhibited by about 73.7% and 89.9% in the presence of 30 and 60 μg/mL LU3′O-GP, respectively, while the inhibition induced by 100 μg/mL FL6C-GP was only 6.7%. The results of this assay demonstrated that LU3′O-GP dramatically inhibited the invasion of ES-2 cells.

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus