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Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus

Effects of P. crispus flavonoids on ES-2 cell migration.Cells were exposed to the indicated concentrations of luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) and flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their migration was quantified using a Transwell chamber. Values represent the means of triplicate experiments. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
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pone.0130685.g006: Effects of P. crispus flavonoids on ES-2 cell migration.Cells were exposed to the indicated concentrations of luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) and flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their migration was quantified using a Transwell chamber. Values represent the means of triplicate experiments. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.

Mentions: Cell motility is considered to be an important determinant of the metastatic potential of cancer cells. The effect of LU3′O-GP on the motility of ES-2 cancer cells was examined using a cell migration assay. As shown in Fig 6, the migration of ES-2 cells was significantly decreased in a concentration-dependent manner after exposure to LU3′O-GP. This inhibition was about 67.7% and 88.1%, as compared with control cells, in the presence of 30 and 60 μg/mL LU3′O-GP, respectively. The cells treated with FL6C-GP showed no significant change in migration activity, as compared with control cells.


Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Effects of P. crispus flavonoids on ES-2 cell migration.Cells were exposed to the indicated concentrations of luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) and flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their migration was quantified using a Transwell chamber. Values represent the means of triplicate experiments. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476667&req=5

pone.0130685.g006: Effects of P. crispus flavonoids on ES-2 cell migration.Cells were exposed to the indicated concentrations of luteolin-3′-O-β-d-glucopyranoside (LU3′O-GP) and flavone-6-C-β-d-glucopyranoside (FL6C-GP), and their migration was quantified using a Transwell chamber. Values represent the means of triplicate experiments. *p < 0.01, as compared to the control; #p < 0.05, as compared with the indicated concentration.
Mentions: Cell motility is considered to be an important determinant of the metastatic potential of cancer cells. The effect of LU3′O-GP on the motility of ES-2 cancer cells was examined using a cell migration assay. As shown in Fig 6, the migration of ES-2 cells was significantly decreased in a concentration-dependent manner after exposure to LU3′O-GP. This inhibition was about 67.7% and 88.1%, as compared with control cells, in the presence of 30 and 60 μg/mL LU3′O-GP, respectively. The cells treated with FL6C-GP showed no significant change in migration activity, as compared with control cells.

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus