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Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus

ES-2 cell morphology.ES-2 cells were treated with the indicated P. crispus flavonoids for 48 h, and imaged using an inverted fluorescent microscope.
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pone.0130685.g003: ES-2 cell morphology.ES-2 cells were treated with the indicated P. crispus flavonoids for 48 h, and imaged using an inverted fluorescent microscope.

Mentions: Morphological examinations were conducted to further investigate the effect of LU3′O-GP on ES-2 cells. The ES-2 cells were treated with concentrations of LU3′O-GP associated with low cytotoxicity (30 and 60 μg/mL), or 100 μg/mL FL6C-GP. Images obtained by inverted microscopy after 48-h incubations are shown in Fig 3. The control and FL6C-GP comparison cells showed the classical spindle cell pattern observed in ovarian tumor cells. The cells treated with LU3′O-GP showed a significant loss of mobility and changes in cell morphology; the major morphological change was to the cytomere shape, which became spherical, with a shortened tentacle. These changes occurred in a concentration-dependent manner. The FL6C-GP treatment produced no significant change, as compared with control cells. These results indicated that LU3′O-GP could change the cellular morphology of ES-2 cells and suggested its potential influence on the cell mobility.


Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

ES-2 cell morphology.ES-2 cells were treated with the indicated P. crispus flavonoids for 48 h, and imaged using an inverted fluorescent microscope.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476667&req=5

pone.0130685.g003: ES-2 cell morphology.ES-2 cells were treated with the indicated P. crispus flavonoids for 48 h, and imaged using an inverted fluorescent microscope.
Mentions: Morphological examinations were conducted to further investigate the effect of LU3′O-GP on ES-2 cells. The ES-2 cells were treated with concentrations of LU3′O-GP associated with low cytotoxicity (30 and 60 μg/mL), or 100 μg/mL FL6C-GP. Images obtained by inverted microscopy after 48-h incubations are shown in Fig 3. The control and FL6C-GP comparison cells showed the classical spindle cell pattern observed in ovarian tumor cells. The cells treated with LU3′O-GP showed a significant loss of mobility and changes in cell morphology; the major morphological change was to the cytomere shape, which became spherical, with a shortened tentacle. These changes occurred in a concentration-dependent manner. The FL6C-GP treatment produced no significant change, as compared with control cells. These results indicated that LU3′O-GP could change the cellular morphology of ES-2 cells and suggested its potential influence on the cell mobility.

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus