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Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus

Proliferation inhibition of two P. crispus flavonoids on ES-2 cells.Cells were incubated with five different flavonoid concentrations or dimethyl sulfoxide (DMSO; control) for 48 h. Data represent the means of triplicate experiments ± standard deviation (SD) * p < 0.01.
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pone.0130685.g002: Proliferation inhibition of two P. crispus flavonoids on ES-2 cells.Cells were incubated with five different flavonoid concentrations or dimethyl sulfoxide (DMSO; control) for 48 h. Data represent the means of triplicate experiments ± standard deviation (SD) * p < 0.01.

Mentions: ES-2 ovarian cancer cells were incubated for 48 h with five different concentrations of LU3′O-GP or FL6C-GP. The MTT assay results indicated a concentration-dependent decrease in cell viability in the presence of LU3′O-GP (Fig 2). Cell viability decreased by 63.8% and 73.6% after 48 h exposure to 120 and 240 μg/mL LU3′O-GP, respectively, and the IC50 value was 57.1 μg/mL. The same concentrations of FL6C-GP had a significantly lower impact on cell viability and this compound had an IC50 value of 182.7 μg/mL. These results indicated that LU3′O-GP inhibited the proliferation of ES-2 cells in a concentration-dependent manner.


Effects of Flavonoids from Potamogeton crispus L. on Proliferation, Migration, and Invasion of Human Ovarian Cancer Cells.

Du Y, Feng J, Wang R, Zhang H, Liu J - PLoS ONE (2015)

Proliferation inhibition of two P. crispus flavonoids on ES-2 cells.Cells were incubated with five different flavonoid concentrations or dimethyl sulfoxide (DMSO; control) for 48 h. Data represent the means of triplicate experiments ± standard deviation (SD) * p < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476667&req=5

pone.0130685.g002: Proliferation inhibition of two P. crispus flavonoids on ES-2 cells.Cells were incubated with five different flavonoid concentrations or dimethyl sulfoxide (DMSO; control) for 48 h. Data represent the means of triplicate experiments ± standard deviation (SD) * p < 0.01.
Mentions: ES-2 ovarian cancer cells were incubated for 48 h with five different concentrations of LU3′O-GP or FL6C-GP. The MTT assay results indicated a concentration-dependent decrease in cell viability in the presence of LU3′O-GP (Fig 2). Cell viability decreased by 63.8% and 73.6% after 48 h exposure to 120 and 240 μg/mL LU3′O-GP, respectively, and the IC50 value was 57.1 μg/mL. The same concentrations of FL6C-GP had a significantly lower impact on cell viability and this compound had an IC50 value of 182.7 μg/mL. These results indicated that LU3′O-GP inhibited the proliferation of ES-2 cells in a concentration-dependent manner.

Bottom Line: The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated.Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells.Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

View Article: PubMed Central - PubMed

Affiliation: Institute of Environmental Research, Shandong University, Jinan, 250100, China.

ABSTRACT
In order to explore the efficient utilization of plant resources from constructed wetlands, the potential anti-metastatic effects of flavonoids from Potamogeton crispus L. were investigated in human ovarian cancer cells (ES-2). Two major flavonoids, luteolin-3'-O-β-D-glucopyranoside and flavone-6-C-β-D-glucopyranoside, were isolated from P. crispus and identified. The effects of these flavonoids on cell proliferation, cell morphology, cell cycle, apoptosis, and cell migration and invasion were then investigated. Furthermore, reverse transcriptase polymerase chain reaction assays and western blotting analysis were conducted to examine the expression level of mRNA and protein. Results indicated that Luteolin-3'-O-β-D-glucopyranoside inhibited ES-2 cell migration and invasion and suppressed the expression of two matrix metalloproteinases (MMPs), MMP-2 and MMP-9, and Flavone-6-C-β-D-glucopyranoside had no significant inhibitory effects on ES-2 cells. Thus, this study demonstrated the potential anti-metastatic properties of a P. crispus flavonoid, and provided a scientific approach for the screening of promising natural resources from constructed wetlands to identify useful products for use in the pharmaceutical and healthcare industries.

No MeSH data available.


Related in: MedlinePlus