Limits...
Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus

Identification of adherent cells with immunofluorescence staining.Cell suspensions were prepared by adding 8.74×107 tumor cells from the same patient to the salvaged blood. Adherent cells were stained with AE1/AE3-PE and CD133-FITC. Adherent cells (both AE1/AE3+ and CD133+) that were characterized by invasive growth were found in 85% of samples (A), but none after the M-LDF treatment (B).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476620&req=5

pone.0130864.g006: Identification of adherent cells with immunofluorescence staining.Cell suspensions were prepared by adding 8.74×107 tumor cells from the same patient to the salvaged blood. Adherent cells were stained with AE1/AE3-PE and CD133-FITC. Adherent cells (both AE1/AE3+ and CD133+) that were characterized by invasive growth were found in 85% of samples (A), but none after the M-LDF treatment (B).

Mentions: The filtrate contained as high as 8.74×107 tumor cells (range from 0.26 to 294.00 ×107) / 250 ml of salvaged blood, M-LDF removed up to 4 log of nucleated cells. AE1/AE3+ cells were found in all untreated-samples, and invasive growth cells were found in 85% of untreated-samples (n = 7, Fig 6). They were absent after M-LDF filtration.


Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Identification of adherent cells with immunofluorescence staining.Cell suspensions were prepared by adding 8.74×107 tumor cells from the same patient to the salvaged blood. Adherent cells were stained with AE1/AE3-PE and CD133-FITC. Adherent cells (both AE1/AE3+ and CD133+) that were characterized by invasive growth were found in 85% of samples (A), but none after the M-LDF treatment (B).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476620&req=5

pone.0130864.g006: Identification of adherent cells with immunofluorescence staining.Cell suspensions were prepared by adding 8.74×107 tumor cells from the same patient to the salvaged blood. Adherent cells were stained with AE1/AE3-PE and CD133-FITC. Adherent cells (both AE1/AE3+ and CD133+) that were characterized by invasive growth were found in 85% of samples (A), but none after the M-LDF treatment (B).
Mentions: The filtrate contained as high as 8.74×107 tumor cells (range from 0.26 to 294.00 ×107) / 250 ml of salvaged blood, M-LDF removed up to 4 log of nucleated cells. AE1/AE3+ cells were found in all untreated-samples, and invasive growth cells were found in 85% of untreated-samples (n = 7, Fig 6). They were absent after M-LDF filtration.

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus