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Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus

Ultrastructure of the M-LDF membrane.After filtration, the first layer membrane of M-LDF was examined by light microscopy (A) and transmission electron microscopy (B). A: Nucleated cells were mostly trapped in the superior 1/3 part of the membrane. B: The trapped cells were ruptured with cytoplasm leakage.
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pone.0130864.g005: Ultrastructure of the M-LDF membrane.After filtration, the first layer membrane of M-LDF was examined by light microscopy (A) and transmission electron microscopy (B). A: Nucleated cells were mostly trapped in the superior 1/3 part of the membrane. B: The trapped cells were ruptured with cytoplasm leakage.

Mentions: There were 30 patients in Protocol 2 (Table 1). Nucleated cell counts in salvaged blood decreased from (3.34±1.28)×109/L of baseline to (5.17±3.78) ×105/L after M-LDF treatment (P = 0.000), while 96% of RBCs were recovered. M-LDF filtration eliminated both CK19+ and CK19+CD133+ cells (Fig 2). The adherent cells, CK19+ and CD133+, were characterized by invasive growth in 70% of untreated samples. They were also eliminated by M-LDF filtration (n = 7, Fig 3). Cells that passed through the M-LDF became naked (Fig 4). Ultrastructure of the M-LDF membrane showed ruptured cell membranes and cytoplasm leakage (Fig 5).


Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Ultrastructure of the M-LDF membrane.After filtration, the first layer membrane of M-LDF was examined by light microscopy (A) and transmission electron microscopy (B). A: Nucleated cells were mostly trapped in the superior 1/3 part of the membrane. B: The trapped cells were ruptured with cytoplasm leakage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476620&req=5

pone.0130864.g005: Ultrastructure of the M-LDF membrane.After filtration, the first layer membrane of M-LDF was examined by light microscopy (A) and transmission electron microscopy (B). A: Nucleated cells were mostly trapped in the superior 1/3 part of the membrane. B: The trapped cells were ruptured with cytoplasm leakage.
Mentions: There were 30 patients in Protocol 2 (Table 1). Nucleated cell counts in salvaged blood decreased from (3.34±1.28)×109/L of baseline to (5.17±3.78) ×105/L after M-LDF treatment (P = 0.000), while 96% of RBCs were recovered. M-LDF filtration eliminated both CK19+ and CK19+CD133+ cells (Fig 2). The adherent cells, CK19+ and CD133+, were characterized by invasive growth in 70% of untreated samples. They were also eliminated by M-LDF filtration (n = 7, Fig 3). Cells that passed through the M-LDF became naked (Fig 4). Ultrastructure of the M-LDF membrane showed ruptured cell membranes and cytoplasm leakage (Fig 5).

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus