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Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus

Flow cytometry and confocal microscopy for identification of viable HepG2 cells.Cells treated with LDF or M-LDF were stained with PI and calcein-AM for viability assessment. Viable cells were found in the LDF group (white arrow, A and C), but not in the M-LDF group (B and D).
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pone.0130864.g001: Flow cytometry and confocal microscopy for identification of viable HepG2 cells.Cells treated with LDF or M-LDF were stained with PI and calcein-AM for viability assessment. Viable cells were found in the LDF group (white arrow, A and C), but not in the M-LDF group (B and D).

Mentions: HepG2 cell count was (7.55±0.63)×106 cells/200 ml before filtration. The count decreased to (2±4) cells/200 ml in the M-LDF group, and to (1.39±2.90) ×103 cells/200 ml in the LDF group. No viable cells or cell colonies were found in M-LDF-treated samples, except fragmented debris (Fig 1), suggesting cells were damaged when they passed through the filter. However, both viable cells (100%) (Fig 1) and cell colonies (37.5%)(S1 Fig) were found in LDF samples.


Modified Leukocyte Filter Removes Tumor Cells from the Salvaged Blood.

Mei K, Du L, Yan M, Zhang Z, Zhang F, Gong L, Sun K, Zhang J, Tang Y, Jiang C, Liu J - PLoS ONE (2015)

Flow cytometry and confocal microscopy for identification of viable HepG2 cells.Cells treated with LDF or M-LDF were stained with PI and calcein-AM for viability assessment. Viable cells were found in the LDF group (white arrow, A and C), but not in the M-LDF group (B and D).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476620&req=5

pone.0130864.g001: Flow cytometry and confocal microscopy for identification of viable HepG2 cells.Cells treated with LDF or M-LDF were stained with PI and calcein-AM for viability assessment. Viable cells were found in the LDF group (white arrow, A and C), but not in the M-LDF group (B and D).
Mentions: HepG2 cell count was (7.55±0.63)×106 cells/200 ml before filtration. The count decreased to (2±4) cells/200 ml in the M-LDF group, and to (1.39±2.90) ×103 cells/200 ml in the LDF group. No viable cells or cell colonies were found in M-LDF-treated samples, except fragmented debris (Fig 1), suggesting cells were damaged when they passed through the filter. However, both viable cells (100%) (Fig 1) and cell colonies (37.5%)(S1 Fig) were found in LDF samples.

Bottom Line: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination.Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample.Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Oncology, Sichuan Cancer Hospital, Chengdu, Sichuan, China.

ABSTRACT

Background: Intraoperative blood salvage, an effective blood conservation strategy, has not been applied in onco-surgery, because of potential malignant cell contamination. In this study we tested effectiveness of a modified leukocyte depletion filter (M-LDF) for removal of tumor cells.

Materials and methods: The effects of M-LDF and regular LDF on removal of cells (HepG2 cell line) were compared. The safety of M-LDF was tested with blood (collected and washed during onco-surgery), the salvaged blood mixed with tumor cells from the solid tumor of the same patient, or mixed with HepG2 cells (n=30 in each protocol). Cancer cells were identified by flow cytometry, culture and bioassay with and without filtration.

Results: M-LDF removed 5-log of HepG2 and nucleated cells, which was much higher than regular LDF, and cells were destroyed when they passed through M-LDF. Cytokeratin-positive cells in all samples were removed by M-LDF. Invasive growth adherent cells were found in most of unfiltered samples and 67% of the inoculated nude mice developed tumors in LDF-treated sample. Neither adherent cells nor nude mice developed tumors were found in M-LDF-treated samples.

Discussion and conclusion: Since M-LDF can effectively remove and destroy cancer cells in the salvaged blood, it has great potential for clinical application.

No MeSH data available.


Related in: MedlinePlus