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Targeting Hormone-Related Pathways to Improve Grain Yield in Rice: A Chemical Approach.

Tamaki H, Reguera M, Abdel-Tawab YM, Takebayashi Y, Kasahara H, Blumwald E - PLoS ONE (2015)

Bottom Line: NDGA inhibited the expression of ABA-responsive gene, but did not significantly decrease ABA content.Instead, NDGA significantly decreased jasmonic acid and jasmonic acid-isoleucine.Our results support the notion that the specific inhibition of jasmonic acid and ethylene biosynthesis resulted in grain yield increase in rice.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California Davis, Davis, California 95616, United States of America; Health and Crop Sciences Research Laboratory, Sumitomo Chemical Co. Ltd., Hyogo 665-8555, Japan.

ABSTRACT
Sink/source relationships, regulating the mobilization of stored carbohydrates from the vegetative tissues to the grains, are of key importance for grain filling and grain yield. We used different inhibitors of plant hormone action to assess their effects on grain yield and on the expression of hormone-associated genes. Among the tested chemicals, 2-indol-3-yl-4-oxo-4-phenylbutanoic acid (PEO-IAA; antagonist of auxin receptor), nordihydroguaiaretic acid (NDGA; abscisic acid (ABA) biosynthesis inhibitor), and 2-aminoisobutyric acid (AIB; ethylene biosynthesis inhibitor) improved grain yield in a concentration dependent manner. These effects were also dependent on the plant developmental stage. NDGA and AIB treatments induced an increase in photosynthesis in flag leaves concomitant to the increments of starch content in flag leaves and grains. NDGA inhibited the expression of ABA-responsive gene, but did not significantly decrease ABA content. Instead, NDGA significantly decreased jasmonic acid and jasmonic acid-isoleucine. Our results support the notion that the specific inhibition of jasmonic acid and ethylene biosynthesis resulted in grain yield increase in rice.

No MeSH data available.


Effects of ABA and Ethylene biosynthesis inhibitors on photosynthesis, conductance, and transpiration rates at pre-anthesis.Photosynthesis rate (A, D), Conductance (B, E), and Transpiration rate (C, F) of UTC and NDGA treated plants (A, B, C) or UTC and AIB treated plants (D, E, F) 2, 9, and 16 d after spray at pre-anthesis stage. Values are Mean ± SE (n = 6). * indicating significance at P ≤ 0.05.
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pone.0131213.g001: Effects of ABA and Ethylene biosynthesis inhibitors on photosynthesis, conductance, and transpiration rates at pre-anthesis.Photosynthesis rate (A, D), Conductance (B, E), and Transpiration rate (C, F) of UTC and NDGA treated plants (A, B, C) or UTC and AIB treated plants (D, E, F) 2, 9, and 16 d after spray at pre-anthesis stage. Values are Mean ± SE (n = 6). * indicating significance at P ≤ 0.05.

Mentions: Photosynthesis and transpiration rates of rice flag leaves improved significantly 2 d after application of low NDGA concentration (Fig 1A and 1C). Higher transpiration and photosynthetic rates were maintained 9 and 16 d after treatment, respectively (Fig 1A and 1C). When NDGA was applied at the intermediate and higher concentrations, transpiration and conductance were significantly improved 2 d after application (Fig 1B and 1C). The low and intermediate AIB concentrations caused improvements on the photosynthetic and transpiration rates (Fig 1D and 1F). AIB treatments also improved photosynthesis and transpiration rate when applied at post-anthesis (S6 Fig). These results suggested that treatments with NDGA or AIB can increase the photosynthetic activity and leaf transpiration rates leading to increases of GY and SDW.


Targeting Hormone-Related Pathways to Improve Grain Yield in Rice: A Chemical Approach.

Tamaki H, Reguera M, Abdel-Tawab YM, Takebayashi Y, Kasahara H, Blumwald E - PLoS ONE (2015)

Effects of ABA and Ethylene biosynthesis inhibitors on photosynthesis, conductance, and transpiration rates at pre-anthesis.Photosynthesis rate (A, D), Conductance (B, E), and Transpiration rate (C, F) of UTC and NDGA treated plants (A, B, C) or UTC and AIB treated plants (D, E, F) 2, 9, and 16 d after spray at pre-anthesis stage. Values are Mean ± SE (n = 6). * indicating significance at P ≤ 0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476611&req=5

pone.0131213.g001: Effects of ABA and Ethylene biosynthesis inhibitors on photosynthesis, conductance, and transpiration rates at pre-anthesis.Photosynthesis rate (A, D), Conductance (B, E), and Transpiration rate (C, F) of UTC and NDGA treated plants (A, B, C) or UTC and AIB treated plants (D, E, F) 2, 9, and 16 d after spray at pre-anthesis stage. Values are Mean ± SE (n = 6). * indicating significance at P ≤ 0.05.
Mentions: Photosynthesis and transpiration rates of rice flag leaves improved significantly 2 d after application of low NDGA concentration (Fig 1A and 1C). Higher transpiration and photosynthetic rates were maintained 9 and 16 d after treatment, respectively (Fig 1A and 1C). When NDGA was applied at the intermediate and higher concentrations, transpiration and conductance were significantly improved 2 d after application (Fig 1B and 1C). The low and intermediate AIB concentrations caused improvements on the photosynthetic and transpiration rates (Fig 1D and 1F). AIB treatments also improved photosynthesis and transpiration rate when applied at post-anthesis (S6 Fig). These results suggested that treatments with NDGA or AIB can increase the photosynthetic activity and leaf transpiration rates leading to increases of GY and SDW.

Bottom Line: NDGA inhibited the expression of ABA-responsive gene, but did not significantly decrease ABA content.Instead, NDGA significantly decreased jasmonic acid and jasmonic acid-isoleucine.Our results support the notion that the specific inhibition of jasmonic acid and ethylene biosynthesis resulted in grain yield increase in rice.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Sciences, University of California Davis, Davis, California 95616, United States of America; Health and Crop Sciences Research Laboratory, Sumitomo Chemical Co. Ltd., Hyogo 665-8555, Japan.

ABSTRACT
Sink/source relationships, regulating the mobilization of stored carbohydrates from the vegetative tissues to the grains, are of key importance for grain filling and grain yield. We used different inhibitors of plant hormone action to assess their effects on grain yield and on the expression of hormone-associated genes. Among the tested chemicals, 2-indol-3-yl-4-oxo-4-phenylbutanoic acid (PEO-IAA; antagonist of auxin receptor), nordihydroguaiaretic acid (NDGA; abscisic acid (ABA) biosynthesis inhibitor), and 2-aminoisobutyric acid (AIB; ethylene biosynthesis inhibitor) improved grain yield in a concentration dependent manner. These effects were also dependent on the plant developmental stage. NDGA and AIB treatments induced an increase in photosynthesis in flag leaves concomitant to the increments of starch content in flag leaves and grains. NDGA inhibited the expression of ABA-responsive gene, but did not significantly decrease ABA content. Instead, NDGA significantly decreased jasmonic acid and jasmonic acid-isoleucine. Our results support the notion that the specific inhibition of jasmonic acid and ethylene biosynthesis resulted in grain yield increase in rice.

No MeSH data available.