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Transporting Cells in Semi-Solid Gel Condition and at Ambient Temperature.

Wang J, Chen P, Xu J, Zou JX, Wang H, Chen HW - PLoS ONE (2015)

Bottom Line: The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use.Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen.Upon arrival, it was found that over 90% of the cells were viable and grew well after replating.

View Article: PubMed Central - PubMed

Affiliation: Comprehensive Cancer Center, University of California Davis, Sacramento, California, 95817, United States of America; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California, 95817, United States of America.

ABSTRACT
Mammalian cells including human cancer cells are usually transported in cryovials on dry ice or in a liquid nitrogen vapor shipping vessel between different places at long distance. The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use. In this study, we tested the viability and properties of cells after being preserved or shipped over long distance in Matrigel mixture for different days. Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen. We also sent cells in the Matrigel mixture via FedEx service to different places at ambient temperature. Upon arrival, it was found that over 90% of the cells were viable and grew well after replating. These data collectively suggested that our Matrigel-based method was highly convenient for shipping live cells for long distances in semi-solid gel condition and at ambient temperature.

No MeSH data available.


Related in: MedlinePlus

A flowchart of the Matrigel based method for transporting cells in semi-solid gel condition and at ambient temperature.
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pone.0128229.g001: A flowchart of the Matrigel based method for transporting cells in semi-solid gel condition and at ambient temperature.

Mentions: Matrigel is a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is composed of primarily proteins such as as laminin, entactin, collagen, and proteoglycan with cell adhesive peptide sequences, and small amount of growth factors, which together promotes the survival of many type of cells [5]. Matrigel is widely used in culturing cells for their optimal growth under certain conditions, in cell invasion assays, and in establishment of xenograft tumors in immuno-deficient mice [5–8]. Matrigel can be frozen at -20°C or lower temperatures, liquefied in a 4°C refrigerator or on wet ice, and solidified at above 10°C. Given these properties of Matrigel, here we have developed a Matrigel-based method for transporting cells in semi-solid gel condition and at ambient temperature (Fig 1).


Transporting Cells in Semi-Solid Gel Condition and at Ambient Temperature.

Wang J, Chen P, Xu J, Zou JX, Wang H, Chen HW - PLoS ONE (2015)

A flowchart of the Matrigel based method for transporting cells in semi-solid gel condition and at ambient temperature.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476595&req=5

pone.0128229.g001: A flowchart of the Matrigel based method for transporting cells in semi-solid gel condition and at ambient temperature.
Mentions: Matrigel is a gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It is composed of primarily proteins such as as laminin, entactin, collagen, and proteoglycan with cell adhesive peptide sequences, and small amount of growth factors, which together promotes the survival of many type of cells [5]. Matrigel is widely used in culturing cells for their optimal growth under certain conditions, in cell invasion assays, and in establishment of xenograft tumors in immuno-deficient mice [5–8]. Matrigel can be frozen at -20°C or lower temperatures, liquefied in a 4°C refrigerator or on wet ice, and solidified at above 10°C. Given these properties of Matrigel, here we have developed a Matrigel-based method for transporting cells in semi-solid gel condition and at ambient temperature (Fig 1).

Bottom Line: The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use.Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen.Upon arrival, it was found that over 90% of the cells were viable and grew well after replating.

View Article: PubMed Central - PubMed

Affiliation: Comprehensive Cancer Center, University of California Davis, Sacramento, California, 95817, United States of America; Department of Biochemistry and Molecular Medicine, School of Medicine, University of California Davis, Sacramento, California, 95817, United States of America.

ABSTRACT
Mammalian cells including human cancer cells are usually transported in cryovials on dry ice or in a liquid nitrogen vapor shipping vessel between different places at long distance. The hazardous nature of dry ice and liquid nitrogen, and the associated high shipping cost strongly limit their routine use. In this study, we tested the viability and properties of cells after being preserved or shipped over long distance in Matrigel mixture for different days. Our results showed that cells mixed with Matrigel at suitable ratios maintained excellent viability (>90%) for one week at room temperature and preserved the properties such as morphology, drug sensitivity and metabolism well, which was comparable to cells cryopreserved in liquid nitrogen. We also sent cells in the Matrigel mixture via FedEx service to different places at ambient temperature. Upon arrival, it was found that over 90% of the cells were viable and grew well after replating. These data collectively suggested that our Matrigel-based method was highly convenient for shipping live cells for long distances in semi-solid gel condition and at ambient temperature.

No MeSH data available.


Related in: MedlinePlus