Limits...
Minimally Invasive Monitoring of Chronic Central Venous Catheter Patency in Mice Using Digital Subtraction Angiography (DSA).

Figueiredo G, Fiebig T, Kirschner S, Nikoubashman O, Kabelitz L, Othman A, Nonn A, Kramer M, Brockmann MA - PLoS ONE (2015)

Bottom Line: The introduction of vascular access mini-ports (VAMP) for mice allows long-term vascular catheterization, hereby eliminating the need for repeated vessel puncture.At this time point, nevertheless, all VAMPs verified intravascular contrast administration.From our observations we conclude DSA to be a fast and valuable minimally invasive tool for investigation of catheter and parent vessel patency and for anatomical studies of collateral blood flow in animals as small as mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic and Interventional Neuroradiology, University Hospital of the RWTH Aachen, Aachen, Germany; Department of Neuroradiology, University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.

ABSTRACT

Background: Repetitive administration of medication or contrast agents is frequently performed in mice. The introduction of vascular access mini-ports (VAMP) for mice allows long-term vascular catheterization, hereby eliminating the need for repeated vessel puncture. With catheter occlusion being the most commonly reported complication of chronic jugular vein catheterization, we tested whether digital subtraction angiography (DSA) can be utilized to evaluate VAMP patency in mice.

Methods: Twenty-three mice underwent catheterization of the jugular vein and subcutaneous implantation of a VAMP. The VAMP was flushed every second day with 50 μL of heparinized saline solution (25 IU/ml). DSA was performed during injection of 100 μL of an iodine based contrast agent using an industrial X-ray inspection system intraoperatively, as well as 7±2 and 14±2 days post implantation.

Results: DSA allowed localization of catheter tip position, to rule out dislocation, kinking or occlusion of a microcatheter, and to evaluate parent vessel patency. In addition, we observed different ante- and retrograde collateral flow patterns in case of jugular vein occlusion. More exactly, 30% of animals showed parent vessel occlusion after 7±2 days in our setting. At this time point, nevertheless, all VAMPs verified intravascular contrast administration. After 14±2 days, intravascular contrast injection was verified in 70% of the implanted VAMPs, whereas at this point of time 5 animals had died or were sacrificed and in 2 mice parent vessel occlusion hampered intravascular contrast injection. Notably, no occlusion of the catheter itself was observed.

Conclusion: From our observations we conclude DSA to be a fast and valuable minimally invasive tool for investigation of catheter and parent vessel patency and for anatomical studies of collateral blood flow in animals as small as mice.

No MeSH data available.


Related in: MedlinePlus

Collateral antegrade flow pattern.The catheter tip (arrow head) is located within the jugular vein of the mouse. Contrast agent flows from the patent jugular vein into the right atrium (arrow) (A). From there it fills the lungs (B) and the aortic arch (AA) including the supraaortic arterial vessels (C). Contrast injection via the implanted mini-port one week later shows occlusion of the jugular vein (D). Instead, the contrast agent flowed (indicated by dotted arrows) via a “ladder-like” venous collateral network (E) with some delay and reduced contrast intensity into the right atrium (arrow) (F).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476576&req=5

pone.0130661.g002: Collateral antegrade flow pattern.The catheter tip (arrow head) is located within the jugular vein of the mouse. Contrast agent flows from the patent jugular vein into the right atrium (arrow) (A). From there it fills the lungs (B) and the aortic arch (AA) including the supraaortic arterial vessels (C). Contrast injection via the implanted mini-port one week later shows occlusion of the jugular vein (D). Instead, the contrast agent flowed (indicated by dotted arrows) via a “ladder-like” venous collateral network (E) with some delay and reduced contrast intensity into the right atrium (arrow) (F).

Mentions: Angiography was carried out directly after the operation in 23 mice. The catheter tip was localized within the right ventricle (n = 1), right atrium (n = 3), superior cava vein (n = 8) and jugular vein (n = 11). In the course of the study, we observed dislocation of three catheters: two dislocated from the superior cava vein into the right atrium and one, positioned in the superior vena cava, dislocated into the proximal jugular vein. Catheter breakage was ruled out in all cases. DSA directly after implantation of the VAMP showed a regular antegrade blood flow pattern with high contrast of the aorta and supra-aortic vessels as shown exemplarily in Fig 1 (and Figs 2A–2C and 3A–3C).


Minimally Invasive Monitoring of Chronic Central Venous Catheter Patency in Mice Using Digital Subtraction Angiography (DSA).

Figueiredo G, Fiebig T, Kirschner S, Nikoubashman O, Kabelitz L, Othman A, Nonn A, Kramer M, Brockmann MA - PLoS ONE (2015)

Collateral antegrade flow pattern.The catheter tip (arrow head) is located within the jugular vein of the mouse. Contrast agent flows from the patent jugular vein into the right atrium (arrow) (A). From there it fills the lungs (B) and the aortic arch (AA) including the supraaortic arterial vessels (C). Contrast injection via the implanted mini-port one week later shows occlusion of the jugular vein (D). Instead, the contrast agent flowed (indicated by dotted arrows) via a “ladder-like” venous collateral network (E) with some delay and reduced contrast intensity into the right atrium (arrow) (F).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476576&req=5

pone.0130661.g002: Collateral antegrade flow pattern.The catheter tip (arrow head) is located within the jugular vein of the mouse. Contrast agent flows from the patent jugular vein into the right atrium (arrow) (A). From there it fills the lungs (B) and the aortic arch (AA) including the supraaortic arterial vessels (C). Contrast injection via the implanted mini-port one week later shows occlusion of the jugular vein (D). Instead, the contrast agent flowed (indicated by dotted arrows) via a “ladder-like” venous collateral network (E) with some delay and reduced contrast intensity into the right atrium (arrow) (F).
Mentions: Angiography was carried out directly after the operation in 23 mice. The catheter tip was localized within the right ventricle (n = 1), right atrium (n = 3), superior cava vein (n = 8) and jugular vein (n = 11). In the course of the study, we observed dislocation of three catheters: two dislocated from the superior cava vein into the right atrium and one, positioned in the superior vena cava, dislocated into the proximal jugular vein. Catheter breakage was ruled out in all cases. DSA directly after implantation of the VAMP showed a regular antegrade blood flow pattern with high contrast of the aorta and supra-aortic vessels as shown exemplarily in Fig 1 (and Figs 2A–2C and 3A–3C).

Bottom Line: The introduction of vascular access mini-ports (VAMP) for mice allows long-term vascular catheterization, hereby eliminating the need for repeated vessel puncture.At this time point, nevertheless, all VAMPs verified intravascular contrast administration.From our observations we conclude DSA to be a fast and valuable minimally invasive tool for investigation of catheter and parent vessel patency and for anatomical studies of collateral blood flow in animals as small as mice.

View Article: PubMed Central - PubMed

Affiliation: Department of Diagnostic and Interventional Neuroradiology, University Hospital of the RWTH Aachen, Aachen, Germany; Department of Neuroradiology, University Medical Center Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany.

ABSTRACT

Background: Repetitive administration of medication or contrast agents is frequently performed in mice. The introduction of vascular access mini-ports (VAMP) for mice allows long-term vascular catheterization, hereby eliminating the need for repeated vessel puncture. With catheter occlusion being the most commonly reported complication of chronic jugular vein catheterization, we tested whether digital subtraction angiography (DSA) can be utilized to evaluate VAMP patency in mice.

Methods: Twenty-three mice underwent catheterization of the jugular vein and subcutaneous implantation of a VAMP. The VAMP was flushed every second day with 50 μL of heparinized saline solution (25 IU/ml). DSA was performed during injection of 100 μL of an iodine based contrast agent using an industrial X-ray inspection system intraoperatively, as well as 7±2 and 14±2 days post implantation.

Results: DSA allowed localization of catheter tip position, to rule out dislocation, kinking or occlusion of a microcatheter, and to evaluate parent vessel patency. In addition, we observed different ante- and retrograde collateral flow patterns in case of jugular vein occlusion. More exactly, 30% of animals showed parent vessel occlusion after 7±2 days in our setting. At this time point, nevertheless, all VAMPs verified intravascular contrast administration. After 14±2 days, intravascular contrast injection was verified in 70% of the implanted VAMPs, whereas at this point of time 5 animals had died or were sacrificed and in 2 mice parent vessel occlusion hampered intravascular contrast injection. Notably, no occlusion of the catheter itself was observed.

Conclusion: From our observations we conclude DSA to be a fast and valuable minimally invasive tool for investigation of catheter and parent vessel patency and for anatomical studies of collateral blood flow in animals as small as mice.

No MeSH data available.


Related in: MedlinePlus