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Insight into the Migration Routes of Plutella xylostella in China Using mtCOI and ISSR Markers.

Yang J, Tian L, Xu B, Xie W, Wang S, Zhang Y, Wang X, Wu Q - PLoS ONE (2015)

Bottom Line: The dominant haplotypes, LX1 and LX2, differed significantly from all other haplotypes both in terms of the number of individuals with those haplotypes and their distributions.Haplotypes that were shared among populations revealed that P. xylostella migrates from the lower reaches of the Yangtze River to northern China and then to northeastern China.Our results also revealed another potential migration route for P. xylostella, i.e., from southwestern China to both northwestern and southern China.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
The larvae of the diamondback moth, Plutella xylostella, cause major economic losses to cruciferous crops, including cabbage, which is an important vegetable crop in China. In this study, we used the mitochondrial COI gene and 11 ISSR markers to characterize the genetic structure and seasonal migration routes of 23 P. xylostella populations in China. Both the mitochondrial and nuclear markers revealed high haplotype diversity and gene flow among the populations, although some degree of genetic isolation was evident between the populations of Hainan Island and other sampling sites. The dominant haplotypes, LX1 and LX2, differed significantly from all other haplotypes both in terms of the number of individuals with those haplotypes and their distributions. Haplotypes that were shared among populations revealed that P. xylostella migrates from the lower reaches of the Yangtze River to northern China and then to northeastern China. Our results also revealed another potential migration route for P. xylostella, i.e., from southwestern China to both northwestern and southern China.

No MeSH data available.


Comparison of mtCO1 haplotype frequency distributions in samples of Plutella xylostella from South Korean (top) and in Plutella xylostella populations across China (bottom).Haplotypes with frequency ≤ 2 are not shown.
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pone.0130905.g004: Comparison of mtCO1 haplotype frequency distributions in samples of Plutella xylostella from South Korean (top) and in Plutella xylostella populations across China (bottom).Haplotypes with frequency ≤ 2 are not shown.

Mentions: A statistical parsimony network analysis of the relationships among mtCOI haplotypes revealed that haplotype LX1 occupied a central position in all populations. Unique haplotypes were located at the edges of the network (Fig 3). When we compared the sequences of the mtCOI gene with 658-bp homologous regions from GenBank (GenBank accession numbers KC154937–KC155152 and DQ076332–DQ076411), the number of haplotype increased to 324. Two South Korean haplotypes were identical to haplotypes LX37 and LX39 from China, but the most common haplotype in South Korea (LX194) was absent in China. In addition, neither haplotype LX1 nor LX2 was found in South Korea (Fig 4). A neighbor-joining tree constructed for the 324 haplotypes revealed that most haplotypes clustered as a single clade (S1 Fig).


Insight into the Migration Routes of Plutella xylostella in China Using mtCOI and ISSR Markers.

Yang J, Tian L, Xu B, Xie W, Wang S, Zhang Y, Wang X, Wu Q - PLoS ONE (2015)

Comparison of mtCO1 haplotype frequency distributions in samples of Plutella xylostella from South Korean (top) and in Plutella xylostella populations across China (bottom).Haplotypes with frequency ≤ 2 are not shown.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476569&req=5

pone.0130905.g004: Comparison of mtCO1 haplotype frequency distributions in samples of Plutella xylostella from South Korean (top) and in Plutella xylostella populations across China (bottom).Haplotypes with frequency ≤ 2 are not shown.
Mentions: A statistical parsimony network analysis of the relationships among mtCOI haplotypes revealed that haplotype LX1 occupied a central position in all populations. Unique haplotypes were located at the edges of the network (Fig 3). When we compared the sequences of the mtCOI gene with 658-bp homologous regions from GenBank (GenBank accession numbers KC154937–KC155152 and DQ076332–DQ076411), the number of haplotype increased to 324. Two South Korean haplotypes were identical to haplotypes LX37 and LX39 from China, but the most common haplotype in South Korea (LX194) was absent in China. In addition, neither haplotype LX1 nor LX2 was found in South Korea (Fig 4). A neighbor-joining tree constructed for the 324 haplotypes revealed that most haplotypes clustered as a single clade (S1 Fig).

Bottom Line: The dominant haplotypes, LX1 and LX2, differed significantly from all other haplotypes both in terms of the number of individuals with those haplotypes and their distributions.Haplotypes that were shared among populations revealed that P. xylostella migrates from the lower reaches of the Yangtze River to northern China and then to northeastern China.Our results also revealed another potential migration route for P. xylostella, i.e., from southwestern China to both northwestern and southern China.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Agriculture Biological Functional Gene of Heilongjiang Provincial Education Committee, Northeast Agricultural University, Harbin, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

ABSTRACT
The larvae of the diamondback moth, Plutella xylostella, cause major economic losses to cruciferous crops, including cabbage, which is an important vegetable crop in China. In this study, we used the mitochondrial COI gene and 11 ISSR markers to characterize the genetic structure and seasonal migration routes of 23 P. xylostella populations in China. Both the mitochondrial and nuclear markers revealed high haplotype diversity and gene flow among the populations, although some degree of genetic isolation was evident between the populations of Hainan Island and other sampling sites. The dominant haplotypes, LX1 and LX2, differed significantly from all other haplotypes both in terms of the number of individuals with those haplotypes and their distributions. Haplotypes that were shared among populations revealed that P. xylostella migrates from the lower reaches of the Yangtze River to northern China and then to northeastern China. Our results also revealed another potential migration route for P. xylostella, i.e., from southwestern China to both northwestern and southern China.

No MeSH data available.