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The MicroRNA-217 Functions as a Potential Tumor Suppressor in Gastric Cancer by Targeting GPC5.

Wang H, Dong X, Gu X, Qin R, Jia H, Gao J - PLoS ONE (2015)

Bottom Line: Enforced expression of miR-217 inhibited GC cells proliferation and invasion.Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217.In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China.

ABSTRACT
Gastric cancer (GC) is one of the most common malignancies worldwide. Emerging evidence has shown that aberrant expression of microRNAs (miRNAs) plays important roles in cancer progression. However, little is known about the potential role of miR-217 in GC. In this study, we investigated the role of miR-217 on GC cell proliferation and invasion. The expression of miR-217 was down-regulated in GC cells and human GC tissues. Enforced expression of miR-217 inhibited GC cells proliferation and invasion. Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217. In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells. In conclusion, these findings revealed that miR-217 functioned as a tumor suppressor and inhibited the proliferation and invasion of GC cells by targeting GPC5, which might consequently serve as a therapeutic target for GC patients.

No MeSH data available.


Related in: MedlinePlus

Restoration of miR-217 inhibits GPC5-mediated GC cell proliferation and invasion.(A) The cell growth in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using CCK-8 proliferation assay.(B) The cell growth in HGC-27co-transfected with either miR-217 inhibitor or 2.0 μg shGPC5 (knocks down GPC5) or pCDNA empty vector using CCK-8 proliferation assay.(C) The cell invasive in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using invasion assay.(D) The cell invasive in HGC-27co-transfected with either miR-217inhibitor or 2.0 μg shGPC5 or pCDNA empty vector using invasion assay. *p<0.05, ** p<0.01, and ***p<0.001.
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pone.0125474.g005: Restoration of miR-217 inhibits GPC5-mediated GC cell proliferation and invasion.(A) The cell growth in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using CCK-8 proliferation assay.(B) The cell growth in HGC-27co-transfected with either miR-217 inhibitor or 2.0 μg shGPC5 (knocks down GPC5) or pCDNA empty vector using CCK-8 proliferation assay.(C) The cell invasive in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using invasion assay.(D) The cell invasive in HGC-27co-transfected with either miR-217inhibitor or 2.0 μg shGPC5 or pCDNA empty vector using invasion assay. *p<0.05, ** p<0.01, and ***p<0.001.

Mentions: Overexpression of GPC5 promoted the GC cell proliferation and invasion. When miR-217 mimic and pEZ-GPC5 were cotransfected into HGC-27 cells, miR-217 expression reduced the GPC5-induced GC cell proliferation and invasion (Fig 5A and 5C). Inhibition of GPC5 reduced the GC cell proliferation and invasion. When miR-217 inhibitor and shGPC5 were cotransfected into HGC-27 cells, miR-217 inhibitor promoted the shGPC5-inhibited cell proliferation and invasion (Fig 5B and 5D).


The MicroRNA-217 Functions as a Potential Tumor Suppressor in Gastric Cancer by Targeting GPC5.

Wang H, Dong X, Gu X, Qin R, Jia H, Gao J - PLoS ONE (2015)

Restoration of miR-217 inhibits GPC5-mediated GC cell proliferation and invasion.(A) The cell growth in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using CCK-8 proliferation assay.(B) The cell growth in HGC-27co-transfected with either miR-217 inhibitor or 2.0 μg shGPC5 (knocks down GPC5) or pCDNA empty vector using CCK-8 proliferation assay.(C) The cell invasive in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using invasion assay.(D) The cell invasive in HGC-27co-transfected with either miR-217inhibitor or 2.0 μg shGPC5 or pCDNA empty vector using invasion assay. *p<0.05, ** p<0.01, and ***p<0.001.
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Related In: Results  -  Collection

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pone.0125474.g005: Restoration of miR-217 inhibits GPC5-mediated GC cell proliferation and invasion.(A) The cell growth in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using CCK-8 proliferation assay.(B) The cell growth in HGC-27co-transfected with either miR-217 inhibitor or 2.0 μg shGPC5 (knocks down GPC5) or pCDNA empty vector using CCK-8 proliferation assay.(C) The cell invasive in HGC-27co-transfected with either miR-217 mimic or 2.0 μg pEZ-GPC5 or pCDNA empty vector using invasion assay.(D) The cell invasive in HGC-27co-transfected with either miR-217inhibitor or 2.0 μg shGPC5 or pCDNA empty vector using invasion assay. *p<0.05, ** p<0.01, and ***p<0.001.
Mentions: Overexpression of GPC5 promoted the GC cell proliferation and invasion. When miR-217 mimic and pEZ-GPC5 were cotransfected into HGC-27 cells, miR-217 expression reduced the GPC5-induced GC cell proliferation and invasion (Fig 5A and 5C). Inhibition of GPC5 reduced the GC cell proliferation and invasion. When miR-217 inhibitor and shGPC5 were cotransfected into HGC-27 cells, miR-217 inhibitor promoted the shGPC5-inhibited cell proliferation and invasion (Fig 5B and 5D).

Bottom Line: Enforced expression of miR-217 inhibited GC cells proliferation and invasion.Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217.In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China.

ABSTRACT
Gastric cancer (GC) is one of the most common malignancies worldwide. Emerging evidence has shown that aberrant expression of microRNAs (miRNAs) plays important roles in cancer progression. However, little is known about the potential role of miR-217 in GC. In this study, we investigated the role of miR-217 on GC cell proliferation and invasion. The expression of miR-217 was down-regulated in GC cells and human GC tissues. Enforced expression of miR-217 inhibited GC cells proliferation and invasion. Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217. In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells. In conclusion, these findings revealed that miR-217 functioned as a tumor suppressor and inhibited the proliferation and invasion of GC cells by targeting GPC5, which might consequently serve as a therapeutic target for GC patients.

No MeSH data available.


Related in: MedlinePlus