Limits...
The MicroRNA-217 Functions as a Potential Tumor Suppressor in Gastric Cancer by Targeting GPC5.

Wang H, Dong X, Gu X, Qin R, Jia H, Gao J - PLoS ONE (2015)

Bottom Line: Enforced expression of miR-217 inhibited GC cells proliferation and invasion.Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217.In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China.

ABSTRACT
Gastric cancer (GC) is one of the most common malignancies worldwide. Emerging evidence has shown that aberrant expression of microRNAs (miRNAs) plays important roles in cancer progression. However, little is known about the potential role of miR-217 in GC. In this study, we investigated the role of miR-217 on GC cell proliferation and invasion. The expression of miR-217 was down-regulated in GC cells and human GC tissues. Enforced expression of miR-217 inhibited GC cells proliferation and invasion. Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217. In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells. In conclusion, these findings revealed that miR-217 functioned as a tumor suppressor and inhibited the proliferation and invasion of GC cells by targeting GPC5, which might consequently serve as a therapeutic target for GC patients.

No MeSH data available.


Related in: MedlinePlus

MiR-217 is downregulated in GC tissues.(A) The tissues were histological confirmed using H&E staining. (Original magnification, ×100). (B) miR-217 was detected in 50 GC patients by real-time PCR. Data are presented as log 2 T/N of fold change of GC tissues relative to non-tumor adjacent tissues. (C) The expression of miR-217 in GC tissues compared with normal tissues. The expression of miR-217 was normalized to U6 snRNA. (D) The statistical analysis of the association between miRNA level and pTNM stage (I, II, III and IV). *p<0.05, and **p<0.01, ***p<0.001. One-way ANOVA was performed for analysis.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4476558&req=5

pone.0125474.g002: MiR-217 is downregulated in GC tissues.(A) The tissues were histological confirmed using H&E staining. (Original magnification, ×100). (B) miR-217 was detected in 50 GC patients by real-time PCR. Data are presented as log 2 T/N of fold change of GC tissues relative to non-tumor adjacent tissues. (C) The expression of miR-217 in GC tissues compared with normal tissues. The expression of miR-217 was normalized to U6 snRNA. (D) The statistical analysis of the association between miRNA level and pTNM stage (I, II, III and IV). *p<0.05, and **p<0.01, ***p<0.001. One-way ANOVA was performed for analysis.

Mentions: Quantitative RT-PCR was used to examine miR-217 expression in 50 GC tissues and their paired adjacent noncancerous tissues. The representative histological characteristics of GC and its paired adjacent noncancerous tissues were shown in Fig 2A. Among 50 tumor tissues, 44 cases exhibited decreased miR-217 expression compared with the adjacent normal tissues (88%, 44 of 50, Fig 2B). The expression of miR-217 was lower in GC tissues than in adjacent noncancerous tissues (Fig 2C). Moreover, lower levels of miR-217 expression associated with the pTNM stage of GC patients (Fig 2D).


The MicroRNA-217 Functions as a Potential Tumor Suppressor in Gastric Cancer by Targeting GPC5.

Wang H, Dong X, Gu X, Qin R, Jia H, Gao J - PLoS ONE (2015)

MiR-217 is downregulated in GC tissues.(A) The tissues were histological confirmed using H&E staining. (Original magnification, ×100). (B) miR-217 was detected in 50 GC patients by real-time PCR. Data are presented as log 2 T/N of fold change of GC tissues relative to non-tumor adjacent tissues. (C) The expression of miR-217 in GC tissues compared with normal tissues. The expression of miR-217 was normalized to U6 snRNA. (D) The statistical analysis of the association between miRNA level and pTNM stage (I, II, III and IV). *p<0.05, and **p<0.01, ***p<0.001. One-way ANOVA was performed for analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4476558&req=5

pone.0125474.g002: MiR-217 is downregulated in GC tissues.(A) The tissues were histological confirmed using H&E staining. (Original magnification, ×100). (B) miR-217 was detected in 50 GC patients by real-time PCR. Data are presented as log 2 T/N of fold change of GC tissues relative to non-tumor adjacent tissues. (C) The expression of miR-217 in GC tissues compared with normal tissues. The expression of miR-217 was normalized to U6 snRNA. (D) The statistical analysis of the association between miRNA level and pTNM stage (I, II, III and IV). *p<0.05, and **p<0.01, ***p<0.001. One-way ANOVA was performed for analysis.
Mentions: Quantitative RT-PCR was used to examine miR-217 expression in 50 GC tissues and their paired adjacent noncancerous tissues. The representative histological characteristics of GC and its paired adjacent noncancerous tissues were shown in Fig 2A. Among 50 tumor tissues, 44 cases exhibited decreased miR-217 expression compared with the adjacent normal tissues (88%, 44 of 50, Fig 2B). The expression of miR-217 was lower in GC tissues than in adjacent noncancerous tissues (Fig 2C). Moreover, lower levels of miR-217 expression associated with the pTNM stage of GC patients (Fig 2D).

Bottom Line: Enforced expression of miR-217 inhibited GC cells proliferation and invasion.Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217.In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, The Affiliated YanAn Hospital of Kunming Medical University, Kunming, 650051, Yunnan, China.

ABSTRACT
Gastric cancer (GC) is one of the most common malignancies worldwide. Emerging evidence has shown that aberrant expression of microRNAs (miRNAs) plays important roles in cancer progression. However, little is known about the potential role of miR-217 in GC. In this study, we investigated the role of miR-217 on GC cell proliferation and invasion. The expression of miR-217 was down-regulated in GC cells and human GC tissues. Enforced expression of miR-217 inhibited GC cells proliferation and invasion. Moreover, Glypican-5 (GPC5), a new ocncogene, was identified as the potential target of miR-217. In addition, overexpression of miR-217 impaired GPC5-induced promotion of proliferation and invasion in GC cells. In conclusion, these findings revealed that miR-217 functioned as a tumor suppressor and inhibited the proliferation and invasion of GC cells by targeting GPC5, which might consequently serve as a therapeutic target for GC patients.

No MeSH data available.


Related in: MedlinePlus