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Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats.

Suidasari S, Hasegawa T, Yanaka N, Kato N - Springerplus (2015)

Bottom Line: This study was performed to examine the effect of dietary level of vitamin B6 on the concentrations of carnosine and anserine, antioxidants, in the heart of rats.Analysis using UPLC-MS/MS showed that the concentrations of these dipeptides in the 7 and 35 mg pyridoxine HCl/kg groups were significantly higher than those in the 1 mg pyridoxine HCl/kg group, implying the novel role of dietary vitamin B6 as a determinant of the dipeptides favorable for heart.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528 Japan.

ABSTRACT
This study was performed to examine the effect of dietary level of vitamin B6 on the concentrations of carnosine and anserine, antioxidants, in the heart of rats. Analysis using UPLC-MS/MS showed that the concentrations of these dipeptides in the 7 and 35 mg pyridoxine HCl/kg groups were significantly higher than those in the 1 mg pyridoxine HCl/kg group, implying the novel role of dietary vitamin B6 as a determinant of the dipeptides favorable for heart.

No MeSH data available.


Effect of dietary levels of B6 (PN HCl) on concentrations of PLP (A), carnosine (B), and anserine (C) in the heart of rats. Mean ± SE (n = 5 for PLP; n = 4 for carnosine and anserine). For the analysis of carnosine and anserine, the supernatant samples of each two rats from the same group of eight rats were combined to obtain the pooled four samples. Values with different superscript are significantly different by Tukey’s multiple-range test (P < 0.05).
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Fig1: Effect of dietary levels of B6 (PN HCl) on concentrations of PLP (A), carnosine (B), and anserine (C) in the heart of rats. Mean ± SE (n = 5 for PLP; n = 4 for carnosine and anserine). For the analysis of carnosine and anserine, the supernatant samples of each two rats from the same group of eight rats were combined to obtain the pooled four samples. Values with different superscript are significantly different by Tukey’s multiple-range test (P < 0.05).

Mentions: Dietary manipulation did not affect food intake (1, 7, and 35 mg PN HCl/kg groups: 731 ± 10, 787 ± 15, and 772 ± 24 g/6 weeks, respectively, P > 0.05) or final body weight (412 ± 9, 444 ± 9, and 437 ± 13 g, respectively, P > 0.05). Heart weight in the 1, 7, and 35 mg PN HCl/kg groups were 1.14 ± 0.05, 1.20 ± 0.03, and 1.16 ± 0.03 g, respectively, P > 0.05). PLP concentrations in the heart and serum in the 7 and 35 mg PN HCl/kg groups were higher (P < 0.01) than those in the 1 mg PN HCl/kg group (Figures 1A, 2A, respectively). There was no significant difference in the PLP concentrations between the 7 and 35 mg PN HCl/kg groups.Figure 1


Dietary supplemental vitamin B6 increases carnosine and anserine concentrations in the heart of rats.

Suidasari S, Hasegawa T, Yanaka N, Kato N - Springerplus (2015)

Effect of dietary levels of B6 (PN HCl) on concentrations of PLP (A), carnosine (B), and anserine (C) in the heart of rats. Mean ± SE (n = 5 for PLP; n = 4 for carnosine and anserine). For the analysis of carnosine and anserine, the supernatant samples of each two rats from the same group of eight rats were combined to obtain the pooled four samples. Values with different superscript are significantly different by Tukey’s multiple-range test (P < 0.05).
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472654&req=5

Fig1: Effect of dietary levels of B6 (PN HCl) on concentrations of PLP (A), carnosine (B), and anserine (C) in the heart of rats. Mean ± SE (n = 5 for PLP; n = 4 for carnosine and anserine). For the analysis of carnosine and anserine, the supernatant samples of each two rats from the same group of eight rats were combined to obtain the pooled four samples. Values with different superscript are significantly different by Tukey’s multiple-range test (P < 0.05).
Mentions: Dietary manipulation did not affect food intake (1, 7, and 35 mg PN HCl/kg groups: 731 ± 10, 787 ± 15, and 772 ± 24 g/6 weeks, respectively, P > 0.05) or final body weight (412 ± 9, 444 ± 9, and 437 ± 13 g, respectively, P > 0.05). Heart weight in the 1, 7, and 35 mg PN HCl/kg groups were 1.14 ± 0.05, 1.20 ± 0.03, and 1.16 ± 0.03 g, respectively, P > 0.05). PLP concentrations in the heart and serum in the 7 and 35 mg PN HCl/kg groups were higher (P < 0.01) than those in the 1 mg PN HCl/kg group (Figures 1A, 2A, respectively). There was no significant difference in the PLP concentrations between the 7 and 35 mg PN HCl/kg groups.Figure 1

Bottom Line: This study was performed to examine the effect of dietary level of vitamin B6 on the concentrations of carnosine and anserine, antioxidants, in the heart of rats.Analysis using UPLC-MS/MS showed that the concentrations of these dipeptides in the 7 and 35 mg pyridoxine HCl/kg groups were significantly higher than those in the 1 mg pyridoxine HCl/kg group, implying the novel role of dietary vitamin B6 as a determinant of the dipeptides favorable for heart.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, 739-8528 Japan.

ABSTRACT
This study was performed to examine the effect of dietary level of vitamin B6 on the concentrations of carnosine and anserine, antioxidants, in the heart of rats. Analysis using UPLC-MS/MS showed that the concentrations of these dipeptides in the 7 and 35 mg pyridoxine HCl/kg groups were significantly higher than those in the 1 mg pyridoxine HCl/kg group, implying the novel role of dietary vitamin B6 as a determinant of the dipeptides favorable for heart.

No MeSH data available.