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Rhamnetin attenuates cognitive deficit and inhibits hippocampal inflammatory response and oxidative stress in rats with traumatic brain injury.

Zhang W, Li B, Guo Y, Bai Y, Wang T, Fu K, Sun G - Cent Eur J Immunol (2015)

Bottom Line: This study aimed to detect the possible effects of rhamnetin on cognitive deficit, hippocampal inflammatory factors, and oxidative stress in rats with TBI.We found that rhamnetin significantly improved cognitive impairment in rats with TBI, and inhibited the inflammatory response and oxidative stress in the hippocampus.The results suggested that rhamnetin could enhance the recovery of cognitive deficits induced by TBI, and that its mechanism might be associated with the inhibition of inflammation and oxidative stress in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Yidu Central Hospital, Shandong Province, Qingzhou, China.

ABSTRACT
Activation of the immune system in the central nervous system and oxidative stress play important roles in traumatic brain injury (TBI)-induced cognitive impairment. Rhamnetin possesses anti-inflammatory and anti-oxidative properties. This study aimed to detect the possible effects of rhamnetin on cognitive deficit, hippocampal inflammatory factors, and oxidative stress in rats with TBI. In this study, we established the traumatic brain injury model in rats. Rats respectively received vehicle saline or rhamnetin for 21 days. Cognitive functions were evaluated by assessing the acquisition of spatial learning and memory retention in Morris Water Maze test from day 15 to 19 post TBI. Levels of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor a (TNF-a), IL-10, and nuclear factor κB (NF-κB) in hippocampal homogenate were measured using ELISA. Oxidative stress was analysed by investigating the activities of MDA, H2O2, SOD, and GSH-Px. We found that rhamnetin significantly improved cognitive impairment in rats with TBI, and inhibited the inflammatory response and oxidative stress in the hippocampus. The results suggested that rhamnetin could enhance the recovery of cognitive deficits induced by TBI, and that its mechanism might be associated with the inhibition of inflammation and oxidative stress in the hippocampus.

No MeSH data available.


Related in: MedlinePlus

Effects of rhamnetin on hs-CRP Control – control group; Sham – sham group; TBI – TBI group; TBI + L – rhamnetin low-dose group; TBI + M – rhamnetin middle-dose group; TBI + H – rhamnetin high-dose group. The investigations were repeated 3 times and the 3 values of each animal were averaged. Data were expressed as mean ± SD (N = 10 per group) and analyzed using oneway ANOVA with subsequent SNK test. ap < 0.05, vs. the control group; bp < 0.05, vs. the sham group; cp < 0.05, vs. the TBI group
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Figure 0002: Effects of rhamnetin on hs-CRP Control – control group; Sham – sham group; TBI – TBI group; TBI + L – rhamnetin low-dose group; TBI + M – rhamnetin middle-dose group; TBI + H – rhamnetin high-dose group. The investigations were repeated 3 times and the 3 values of each animal were averaged. Data were expressed as mean ± SD (N = 10 per group) and analyzed using oneway ANOVA with subsequent SNK test. ap < 0.05, vs. the control group; bp < 0.05, vs. the sham group; cp < 0.05, vs. the TBI group

Mentions: We found increased hippocampal levels of TNF-α, IL-1β, IL-6, IL-8, hs-CRP, and NF-κB, as well as decreased levels of IL-10, in the rats of the TBI group compared to the control group and the sham group (all p < 0.05). The changes induced by TBI were significantly reversed by rhamnetin in the TBI + M group and the TBI + H group, but not in the TBI + L group, compared to the TBI group. No differences were observed between the control group and the sham group (all p > 0.05). Shown in Figures 1 and 2.


Rhamnetin attenuates cognitive deficit and inhibits hippocampal inflammatory response and oxidative stress in rats with traumatic brain injury.

Zhang W, Li B, Guo Y, Bai Y, Wang T, Fu K, Sun G - Cent Eur J Immunol (2015)

Effects of rhamnetin on hs-CRP Control – control group; Sham – sham group; TBI – TBI group; TBI + L – rhamnetin low-dose group; TBI + M – rhamnetin middle-dose group; TBI + H – rhamnetin high-dose group. The investigations were repeated 3 times and the 3 values of each animal were averaged. Data were expressed as mean ± SD (N = 10 per group) and analyzed using oneway ANOVA with subsequent SNK test. ap < 0.05, vs. the control group; bp < 0.05, vs. the sham group; cp < 0.05, vs. the TBI group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472538&req=5

Figure 0002: Effects of rhamnetin on hs-CRP Control – control group; Sham – sham group; TBI – TBI group; TBI + L – rhamnetin low-dose group; TBI + M – rhamnetin middle-dose group; TBI + H – rhamnetin high-dose group. The investigations were repeated 3 times and the 3 values of each animal were averaged. Data were expressed as mean ± SD (N = 10 per group) and analyzed using oneway ANOVA with subsequent SNK test. ap < 0.05, vs. the control group; bp < 0.05, vs. the sham group; cp < 0.05, vs. the TBI group
Mentions: We found increased hippocampal levels of TNF-α, IL-1β, IL-6, IL-8, hs-CRP, and NF-κB, as well as decreased levels of IL-10, in the rats of the TBI group compared to the control group and the sham group (all p < 0.05). The changes induced by TBI were significantly reversed by rhamnetin in the TBI + M group and the TBI + H group, but not in the TBI + L group, compared to the TBI group. No differences were observed between the control group and the sham group (all p > 0.05). Shown in Figures 1 and 2.

Bottom Line: This study aimed to detect the possible effects of rhamnetin on cognitive deficit, hippocampal inflammatory factors, and oxidative stress in rats with TBI.We found that rhamnetin significantly improved cognitive impairment in rats with TBI, and inhibited the inflammatory response and oxidative stress in the hippocampus.The results suggested that rhamnetin could enhance the recovery of cognitive deficits induced by TBI, and that its mechanism might be associated with the inhibition of inflammation and oxidative stress in the hippocampus.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurosurgery, Yidu Central Hospital, Shandong Province, Qingzhou, China.

ABSTRACT
Activation of the immune system in the central nervous system and oxidative stress play important roles in traumatic brain injury (TBI)-induced cognitive impairment. Rhamnetin possesses anti-inflammatory and anti-oxidative properties. This study aimed to detect the possible effects of rhamnetin on cognitive deficit, hippocampal inflammatory factors, and oxidative stress in rats with TBI. In this study, we established the traumatic brain injury model in rats. Rats respectively received vehicle saline or rhamnetin for 21 days. Cognitive functions were evaluated by assessing the acquisition of spatial learning and memory retention in Morris Water Maze test from day 15 to 19 post TBI. Levels of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor a (TNF-a), IL-10, and nuclear factor κB (NF-κB) in hippocampal homogenate were measured using ELISA. Oxidative stress was analysed by investigating the activities of MDA, H2O2, SOD, and GSH-Px. We found that rhamnetin significantly improved cognitive impairment in rats with TBI, and inhibited the inflammatory response and oxidative stress in the hippocampus. The results suggested that rhamnetin could enhance the recovery of cognitive deficits induced by TBI, and that its mechanism might be associated with the inhibition of inflammation and oxidative stress in the hippocampus.

No MeSH data available.


Related in: MedlinePlus