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The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice.

Zhao Y, Zhang Y, Pan F - Cent Eur J Immunol (2015)

Bottom Line: But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated.We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice.Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Psychology, Shandong University Medical School, Weifang People's Hospital, China.

ABSTRACT
There is an increasing body of evidence for the involvement of inflammation and brain-derived neurotrophic factor (BDNF) in depression. Ginkgo extract EGb761 possesses anti-inflammatory, anti-oxidative, anti-arteriosclerosis, and neuroprotective activities. But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated. The present study mainly aimed to examine the antidepressant-like activities of Ginkgo extract EGb761 in mice after lipopolysaccharide administration. C57BL/6J male mice were pretreated with EGb761 or vehicle for 10 days. Then, a single dose of lipopolysaccharide was intraperitoneally administrated to mice to induce depressive-like behaviour. Forced swim test (FST), tail suspension test (TST), and sucrose preference test were performed to evaluate the depressive-like behaviours of the mice. Locomotor activity was examined by open field test. Levels of brain-derived neurotrophic factor, TNF-α, IL-1β, IL-6, IL-17A, and IL-10 in hippocampus tissue homogenate were measured using ELISA kits. We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice. Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration. We conclude that EGb761 has antidepressant-like activities in mice with LPS-induced depressive-like behaviours.

No MeSH data available.


Related in: MedlinePlus

Effects of EGb761 on immobility time in tail suspension test (TST)
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Figure 0003: Effects of EGb761 on immobility time in tail suspension test (TST)

Mentions: Figure 3 shows the results of the immobility time in tail suspension test (TST). The duration of immobility in TST was significantly elevated by LPS injection in the vehicle group, compared to the control animals (p < 0.05), and this elevation was significantly reversed by EGb761 pre-treatment in the middle-dose group and high-dose group in comparison with the vehicle group (p < 0.05). Moreover, the high-dose group's immobility time was much shorter than that of the low-dose group and the middle-dose group (both p < 0.05), suggesting a dose-related antidepressant-like effect of EGb761.


The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice.

Zhao Y, Zhang Y, Pan F - Cent Eur J Immunol (2015)

Effects of EGb761 on immobility time in tail suspension test (TST)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472532&req=5

Figure 0003: Effects of EGb761 on immobility time in tail suspension test (TST)
Mentions: Figure 3 shows the results of the immobility time in tail suspension test (TST). The duration of immobility in TST was significantly elevated by LPS injection in the vehicle group, compared to the control animals (p < 0.05), and this elevation was significantly reversed by EGb761 pre-treatment in the middle-dose group and high-dose group in comparison with the vehicle group (p < 0.05). Moreover, the high-dose group's immobility time was much shorter than that of the low-dose group and the middle-dose group (both p < 0.05), suggesting a dose-related antidepressant-like effect of EGb761.

Bottom Line: But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated.We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice.Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Psychology, Shandong University Medical School, Weifang People's Hospital, China.

ABSTRACT
There is an increasing body of evidence for the involvement of inflammation and brain-derived neurotrophic factor (BDNF) in depression. Ginkgo extract EGb761 possesses anti-inflammatory, anti-oxidative, anti-arteriosclerosis, and neuroprotective activities. But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated. The present study mainly aimed to examine the antidepressant-like activities of Ginkgo extract EGb761 in mice after lipopolysaccharide administration. C57BL/6J male mice were pretreated with EGb761 or vehicle for 10 days. Then, a single dose of lipopolysaccharide was intraperitoneally administrated to mice to induce depressive-like behaviour. Forced swim test (FST), tail suspension test (TST), and sucrose preference test were performed to evaluate the depressive-like behaviours of the mice. Locomotor activity was examined by open field test. Levels of brain-derived neurotrophic factor, TNF-α, IL-1β, IL-6, IL-17A, and IL-10 in hippocampus tissue homogenate were measured using ELISA kits. We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice. Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration. We conclude that EGb761 has antidepressant-like activities in mice with LPS-induced depressive-like behaviours.

No MeSH data available.


Related in: MedlinePlus