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The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice.

Zhao Y, Zhang Y, Pan F - Cent Eur J Immunol (2015)

Bottom Line: But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated.We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice.Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Psychology, Shandong University Medical School, Weifang People's Hospital, China.

ABSTRACT
There is an increasing body of evidence for the involvement of inflammation and brain-derived neurotrophic factor (BDNF) in depression. Ginkgo extract EGb761 possesses anti-inflammatory, anti-oxidative, anti-arteriosclerosis, and neuroprotective activities. But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated. The present study mainly aimed to examine the antidepressant-like activities of Ginkgo extract EGb761 in mice after lipopolysaccharide administration. C57BL/6J male mice were pretreated with EGb761 or vehicle for 10 days. Then, a single dose of lipopolysaccharide was intraperitoneally administrated to mice to induce depressive-like behaviour. Forced swim test (FST), tail suspension test (TST), and sucrose preference test were performed to evaluate the depressive-like behaviours of the mice. Locomotor activity was examined by open field test. Levels of brain-derived neurotrophic factor, TNF-α, IL-1β, IL-6, IL-17A, and IL-10 in hippocampus tissue homogenate were measured using ELISA kits. We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice. Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration. We conclude that EGb761 has antidepressant-like activities in mice with LPS-induced depressive-like behaviours.

No MeSH data available.


Related in: MedlinePlus

Effects of EGb761 on immobility time in forced swim test
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Figure 0002: Effects of EGb761 on immobility time in forced swim test

Mentions: Immobility time is extensively used to assess the depressive state of rodents. Figure 2 shows the results of the immobility time in forced swim test (FST). The vehicle group showed markedly longer immobility time than the control group (p < 0.05), indicating that LPS injection induced depressive behaviours in mice. However, the middle-dose group and the high-dose group showed shorter immobility time than the vehicle group (both p < 0.05). Moreover, the high-dose group's immobility time was much shorter than that of the low-dose group and the middle-dose group (both p < 0.05). The results suggest that EGb761 pretreatment had an antidepressant-like effect in mice with LPS injection, and the effect was dose related.


The effects of EGb761 on lipopolysaccharide-induced depressive-like behaviour in C57BL/6J mice.

Zhao Y, Zhang Y, Pan F - Cent Eur J Immunol (2015)

Effects of EGb761 on immobility time in forced swim test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472532&req=5

Figure 0002: Effects of EGb761 on immobility time in forced swim test
Mentions: Immobility time is extensively used to assess the depressive state of rodents. Figure 2 shows the results of the immobility time in forced swim test (FST). The vehicle group showed markedly longer immobility time than the control group (p < 0.05), indicating that LPS injection induced depressive behaviours in mice. However, the middle-dose group and the high-dose group showed shorter immobility time than the vehicle group (both p < 0.05). Moreover, the high-dose group's immobility time was much shorter than that of the low-dose group and the middle-dose group (both p < 0.05). The results suggest that EGb761 pretreatment had an antidepressant-like effect in mice with LPS injection, and the effect was dose related.

Bottom Line: But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated.We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice.Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medical Psychology, Shandong University Medical School, Weifang People's Hospital, China.

ABSTRACT
There is an increasing body of evidence for the involvement of inflammation and brain-derived neurotrophic factor (BDNF) in depression. Ginkgo extract EGb761 possesses anti-inflammatory, anti-oxidative, anti-arteriosclerosis, and neuroprotective activities. But the effect of EGb761 on lipopolysaccharide (LPS)-induced depressive-like behaviours has not been investigated. The present study mainly aimed to examine the antidepressant-like activities of Ginkgo extract EGb761 in mice after lipopolysaccharide administration. C57BL/6J male mice were pretreated with EGb761 or vehicle for 10 days. Then, a single dose of lipopolysaccharide was intraperitoneally administrated to mice to induce depressive-like behaviour. Forced swim test (FST), tail suspension test (TST), and sucrose preference test were performed to evaluate the depressive-like behaviours of the mice. Locomotor activity was examined by open field test. Levels of brain-derived neurotrophic factor, TNF-α, IL-1β, IL-6, IL-17A, and IL-10 in hippocampus tissue homogenate were measured using ELISA kits. We found that LPS administration induced significant depressive-like behaviours, higher levels of tumour necrosis factor α (TNF-α), interleukin (IL) 1β, IL-6, and IL-17A, but lower levels of BDNF and IL-10 in hippocampus tissue homogenate of the mice from the vehicle group compared to the control mice. Pretreatment with middle dose (100 mg/kg/day) and high dose (150 mg/kg/day) of EGb761 significantly attenuated depressive-like behaviours without affecting spontaneous locomotor activity, and inhibited the changes of hippocampal cytokines and BDNF induced by LPS administration. We conclude that EGb761 has antidepressant-like activities in mice with LPS-induced depressive-like behaviours.

No MeSH data available.


Related in: MedlinePlus