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Id3 induces an Elk-1-caspase-8-dependent apoptotic pathway in squamous carcinoma cells.

Chen YS, Aubee J, DiVito KA, Zhou H, Zhang W, Chou FP, Simbulan-Rosenthal CM, Rosenthal DS - Cancer Med (2015)

Bottom Line: We found that upon Id3 induction, there was a decrease in cell number under low serum conditions, as well as in soft agar.Furthermore, we show that Id3 synergizes with 5-FU and cisplatin therapies for nonmelanoma skin cancer cells.Our studies have shown a molecular mechanism by which Id3 induces apoptosis in SCC, and this information can potentially be used to develop new treatments for SCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, District of Columbia, 20057.

No MeSH data available.


Related in: MedlinePlus

(A) Heat map showing 16 genes significantly up- or downregulated by Id3 by at least 1.5-fold. (B) RT-PCR was performed to verify genes that were shown to be upregulated by Id3 in microarray analysis. (C) Immunoblot analysis of proteins corresponding to genes upregulated by Id3.
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fig02: (A) Heat map showing 16 genes significantly up- or downregulated by Id3 by at least 1.5-fold. (B) RT-PCR was performed to verify genes that were shown to be upregulated by Id3 in microarray analysis. (C) Immunoblot analysis of proteins corresponding to genes upregulated by Id3.

Mentions: To investigate the molecular pathways by which Id3 mediates caspase-3 activation and apoptosis in A431 cells, microarray analysis was performed. A431/Id3 and A431/Vc ±Tet cells were subjected to microarray analysis. Id3 altered expression levels of 16 genes greater than 1.5-fold, when induced A431/Id3 was compared with the other three groups (Fig. 2A). We verified expression levels of candidate genes and showed that TBK-BP1, Elk-1, and MXD3 were upregulated at the mRNA (Fig. 2B) and protein (Fig. 2C) levels by Id3.


Id3 induces an Elk-1-caspase-8-dependent apoptotic pathway in squamous carcinoma cells.

Chen YS, Aubee J, DiVito KA, Zhou H, Zhang W, Chou FP, Simbulan-Rosenthal CM, Rosenthal DS - Cancer Med (2015)

(A) Heat map showing 16 genes significantly up- or downregulated by Id3 by at least 1.5-fold. (B) RT-PCR was performed to verify genes that were shown to be upregulated by Id3 in microarray analysis. (C) Immunoblot analysis of proteins corresponding to genes upregulated by Id3.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472214&req=5

fig02: (A) Heat map showing 16 genes significantly up- or downregulated by Id3 by at least 1.5-fold. (B) RT-PCR was performed to verify genes that were shown to be upregulated by Id3 in microarray analysis. (C) Immunoblot analysis of proteins corresponding to genes upregulated by Id3.
Mentions: To investigate the molecular pathways by which Id3 mediates caspase-3 activation and apoptosis in A431 cells, microarray analysis was performed. A431/Id3 and A431/Vc ±Tet cells were subjected to microarray analysis. Id3 altered expression levels of 16 genes greater than 1.5-fold, when induced A431/Id3 was compared with the other three groups (Fig. 2A). We verified expression levels of candidate genes and showed that TBK-BP1, Elk-1, and MXD3 were upregulated at the mRNA (Fig. 2B) and protein (Fig. 2C) levels by Id3.

Bottom Line: We found that upon Id3 induction, there was a decrease in cell number under low serum conditions, as well as in soft agar.Furthermore, we show that Id3 synergizes with 5-FU and cisplatin therapies for nonmelanoma skin cancer cells.Our studies have shown a molecular mechanism by which Id3 induces apoptosis in SCC, and this information can potentially be used to develop new treatments for SCC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular & Cellular Biology, Georgetown University, Washington, District of Columbia, 20057.

No MeSH data available.


Related in: MedlinePlus