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Downregulation of microRNA-409-3p promotes aggressiveness and metastasis in colorectal cancer: an indication for personalized medicine.

Liu M, Xu A, Yuan X, Zhang Q, Fang T, Wang W, Li C - J Transl Med (2015)

Bottom Line: Aberrant miR-409-3p expression has been reported in several cancers.However, the clinical significance and functions of miR-409-3p in human CRC were not entirely clear. miR-409-3p expression levels were determined in 45 pairs of primary CRC and their corresponding adjacent non-tumor tissues by qPCR.Notably, we found the NLK could be a potential target of miR-409-3p.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China. mulinliu11@163.com.

ABSTRACT

Background: MicroRNAs play an ess ential role in colorectal cancer development and progression. Aberrant miR-409-3p expression has been reported in several cancers. However, the clinical significance and functions of miR-409-3p in human CRC were not entirely clear.

Methods: miR-409-3p expression levels were determined in 45 pairs of primary CRC and their corresponding adjacent non-tumor tissues by qPCR. The effects of ectopic expression of miR-409-3p on CRC cells proliferation, wound healing, metastasis were investigated by CCK-8, transwell assay and peritoneal spreading nude mice model.

Results: Statistical analysis of clinical cases revealed that low miR-409-3p expression had inclinations towards lager tumor size and local invasion. Ectopic expression of miRNA mimics suggested that miR-409-3p could inhibits the abilities of proliferation, wound healing, metastasis and invasion in CRC cells. Notably, we found the NLK could be a potential target of miR-409-3p.

Conclusion: Our results suggest that miR-409-3p functions as a tumor suppressor by inhibiting the development and metastasis of CRC, suggesting that miR-409-3p is expected to become a new diagnostic marker and a new target of the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus

miR-409-3p inhibits peritoneal spreading in nude mice. a, b Representative images of nude mice injected with indicated cells. c Quantification of the peritoneal nodules is shown in the bar graph. The results are mean ± SD, *P < 0.05.
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Fig6: miR-409-3p inhibits peritoneal spreading in nude mice. a, b Representative images of nude mice injected with indicated cells. c Quantification of the peritoneal nodules is shown in the bar graph. The results are mean ± SD, *P < 0.05.

Mentions: Given that miR-409-3p inhibits metastasis of CRC cells in vitro, we next tested whether ectopic expression of miR-409-3p could influence the tumor growth and metastasis of CRC cells in vivo. To confirm the contribution of miR-409-3p in vivo, we carried out peritoneal spreading in mice models. CRC cells transfected with miR-409 mimics or control mimics were selected and intraperitoneally injected into nude mice, These mice were euthanized 8 weeks after the injection, and the tumor lesions in the peritoneal cavity were counted. Strikingly, the peritoneal nodules were significantly inhibited in miR-409-3p mimics transfected group compared with control group (Figure 6). Thus, these results indicate that miR-409-3p could suppress metastasis of CRC cells in vivo.Figure 6


Downregulation of microRNA-409-3p promotes aggressiveness and metastasis in colorectal cancer: an indication for personalized medicine.

Liu M, Xu A, Yuan X, Zhang Q, Fang T, Wang W, Li C - J Transl Med (2015)

miR-409-3p inhibits peritoneal spreading in nude mice. a, b Representative images of nude mice injected with indicated cells. c Quantification of the peritoneal nodules is shown in the bar graph. The results are mean ± SD, *P < 0.05.
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4472171&req=5

Fig6: miR-409-3p inhibits peritoneal spreading in nude mice. a, b Representative images of nude mice injected with indicated cells. c Quantification of the peritoneal nodules is shown in the bar graph. The results are mean ± SD, *P < 0.05.
Mentions: Given that miR-409-3p inhibits metastasis of CRC cells in vitro, we next tested whether ectopic expression of miR-409-3p could influence the tumor growth and metastasis of CRC cells in vivo. To confirm the contribution of miR-409-3p in vivo, we carried out peritoneal spreading in mice models. CRC cells transfected with miR-409 mimics or control mimics were selected and intraperitoneally injected into nude mice, These mice were euthanized 8 weeks after the injection, and the tumor lesions in the peritoneal cavity were counted. Strikingly, the peritoneal nodules were significantly inhibited in miR-409-3p mimics transfected group compared with control group (Figure 6). Thus, these results indicate that miR-409-3p could suppress metastasis of CRC cells in vivo.Figure 6

Bottom Line: Aberrant miR-409-3p expression has been reported in several cancers.However, the clinical significance and functions of miR-409-3p in human CRC were not entirely clear. miR-409-3p expression levels were determined in 45 pairs of primary CRC and their corresponding adjacent non-tumor tissues by qPCR.Notably, we found the NLK could be a potential target of miR-409-3p.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastrointestinal Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233030, China. mulinliu11@163.com.

ABSTRACT

Background: MicroRNAs play an ess ential role in colorectal cancer development and progression. Aberrant miR-409-3p expression has been reported in several cancers. However, the clinical significance and functions of miR-409-3p in human CRC were not entirely clear.

Methods: miR-409-3p expression levels were determined in 45 pairs of primary CRC and their corresponding adjacent non-tumor tissues by qPCR. The effects of ectopic expression of miR-409-3p on CRC cells proliferation, wound healing, metastasis were investigated by CCK-8, transwell assay and peritoneal spreading nude mice model.

Results: Statistical analysis of clinical cases revealed that low miR-409-3p expression had inclinations towards lager tumor size and local invasion. Ectopic expression of miRNA mimics suggested that miR-409-3p could inhibits the abilities of proliferation, wound healing, metastasis and invasion in CRC cells. Notably, we found the NLK could be a potential target of miR-409-3p.

Conclusion: Our results suggest that miR-409-3p functions as a tumor suppressor by inhibiting the development and metastasis of CRC, suggesting that miR-409-3p is expected to become a new diagnostic marker and a new target of the treatment of CRC.

No MeSH data available.


Related in: MedlinePlus