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The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells.

Liu C, Zhu C, Li J, Zhou P, Chen M, Yang H, Li B - Bone Res (2015)

Bottom Line: However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of collagen-I and aggrecan.The gene expression and protein production of collagen-II did not appear to differ between the two groups.Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University , 188 Shizi St, Suzhou, Jiangsu 215006, China ; Orthopedic Institute, Soochow University , 708 Renmin Rd, Suzhou, Jiangsu 215007, China.

ABSTRACT
Annulus fibrosus (AF) tissue engineering has recently received increasing attention as a treatment for intervertebral disc (IVD) degeneration; however, such engineering remains challenging because of the remarkable complexity of AF tissue. In order to engineer a functional AF replacement, the fabrication of cell-scaffold constructs that mimic the cellular, biochemical and structural features of native AF tissue is critical. In this study, we fabricated aligned fibrous polyurethane scaffolds using an electrospinning technique and used them for culturing AF-derived stem/progenitor cells (AFSCs). Random fibrous scaffolds, also prepared via electrospinning, were used as a control. We compared the morphology, proliferation, gene expression and matrix production of AFSCs on aligned scaffolds and random scaffolds. There was no apparent difference in the attachment or proliferation of cells cultured on aligned scaffolds and random scaffolds. However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of collagen-I and aggrecan. The gene expression and protein production of collagen-II did not appear to differ between the two groups. Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.

No MeSH data available.


Related in: MedlinePlus

Production of collagen-I (a), collagen-II (b) and GAGs (c) by AFSCs cultured on aligned and random scaffolds for 7 days. Measurements were normalised to DNA content.
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fig5: Production of collagen-I (a), collagen-II (b) and GAGs (c) by AFSCs cultured on aligned and random scaffolds for 7 days. Measurements were normalised to DNA content.

Mentions: In addition to gene expression analysis, the production of matrix components, including collagen-I, collagen-II and GAGs, was measured by ELISA. The production of collagen-I by AFSCs cultured on aligned scaffolds was 4.99 ± 0.27 ng·μg−1 DNA, which was markedly higher than that of AFSCs cultured on random scaffolds (3.78 ± 0.37 ng·μg−1 DNA) (Figure 5a). The production of collagen-II by cells on aligned scaffolds was 6.25 ± 0.14 ng·μg−1 DNA, which was similar to that of cells on random scaffolds (6.79 ± 0.18 ng·μg−1 DNA) (Figure 5b). The production of GAGs on aligned and random scaffolds was 10.70 ± 0.30 μg·μg−1 DNA and 5.75 ± 0.24 μg·μg−1 DNA, respectively (Figure 5c).


The effect of the fibre orientation of electrospun scaffolds on the matrix production of rabbit annulus fibrosus-derived stem cells.

Liu C, Zhu C, Li J, Zhou P, Chen M, Yang H, Li B - Bone Res (2015)

Production of collagen-I (a), collagen-II (b) and GAGs (c) by AFSCs cultured on aligned and random scaffolds for 7 days. Measurements were normalised to DNA content.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4472148&req=5

fig5: Production of collagen-I (a), collagen-II (b) and GAGs (c) by AFSCs cultured on aligned and random scaffolds for 7 days. Measurements were normalised to DNA content.
Mentions: In addition to gene expression analysis, the production of matrix components, including collagen-I, collagen-II and GAGs, was measured by ELISA. The production of collagen-I by AFSCs cultured on aligned scaffolds was 4.99 ± 0.27 ng·μg−1 DNA, which was markedly higher than that of AFSCs cultured on random scaffolds (3.78 ± 0.37 ng·μg−1 DNA) (Figure 5a). The production of collagen-II by cells on aligned scaffolds was 6.25 ± 0.14 ng·μg−1 DNA, which was similar to that of cells on random scaffolds (6.79 ± 0.18 ng·μg−1 DNA) (Figure 5b). The production of GAGs on aligned and random scaffolds was 10.70 ± 0.30 μg·μg−1 DNA and 5.75 ± 0.24 μg·μg−1 DNA, respectively (Figure 5c).

Bottom Line: However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of collagen-I and aggrecan.The gene expression and protein production of collagen-II did not appear to differ between the two groups.Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics, The First Affiliated Hospital of Soochow University , 188 Shizi St, Suzhou, Jiangsu 215006, China ; Orthopedic Institute, Soochow University , 708 Renmin Rd, Suzhou, Jiangsu 215007, China.

ABSTRACT
Annulus fibrosus (AF) tissue engineering has recently received increasing attention as a treatment for intervertebral disc (IVD) degeneration; however, such engineering remains challenging because of the remarkable complexity of AF tissue. In order to engineer a functional AF replacement, the fabrication of cell-scaffold constructs that mimic the cellular, biochemical and structural features of native AF tissue is critical. In this study, we fabricated aligned fibrous polyurethane scaffolds using an electrospinning technique and used them for culturing AF-derived stem/progenitor cells (AFSCs). Random fibrous scaffolds, also prepared via electrospinning, were used as a control. We compared the morphology, proliferation, gene expression and matrix production of AFSCs on aligned scaffolds and random scaffolds. There was no apparent difference in the attachment or proliferation of cells cultured on aligned scaffolds and random scaffolds. However, compared to cells on random scaffolds, the AFSCs on aligned scaffolds were more elongated and better aligned, and they exhibited higher gene expression and matrix production of collagen-I and aggrecan. The gene expression and protein production of collagen-II did not appear to differ between the two groups. Together, these findings indicate that aligned fibrous scaffolds may provide a favourable microenvironment for the differentiation of AFSCs into cells similar to outer AF cells, which predominantly produce collagen-I matrix.

No MeSH data available.


Related in: MedlinePlus