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The traditional Tibetan medicine Yukyung Karne exhibits a potent anti-metastatic activity by inhibiting the epithelial to mesenchymal transition and cell migration.

Choedon T, Mathan G, Kumar V - BMC Complement Altern Med (2015)

Bottom Line: Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic action remains elusive.The effect of Yukyung Karne on the expression of extracellular matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and western blotting.Thus, Yukyung Karne may be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to increase their efficacy.

View Article: PubMed Central - PubMed

Affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT

Background: In Traditional Tibetan medicine, Yukyung Karne has been used for the treatment of ovarian cancer. Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic action remains elusive.

Methods: The cytotoxic property of Yukyung Karne was evaluated by crystal violet staining while its ability to induce ceramide production was analyzed by sphingomyelinase assay. The anti-metastatic property was investigated using adhesion, invasion, migration and colony formation assays. The effect of Yukyung Karne on the expression of extracellular matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and western blotting.

Results: Yukyung Karne exhibited a strong anti-metastatic property by significantly reducing the invasion, migration and colony formation ability of ovarian cancer cells. Besides it inhibited the levels of biomarkers involved in epithelial to mesenchymal transition such as down-regulation of vimentin and N-cadherin and up-regulation of epithelial E-cadherin. Yukyung Karne also induced the neutral sphingomyelinase II (nSMNaseII) enzyme activity that is known to hydrolyze sphingomyelins into pro-apoptotic intracellular molecule ceramide.

Conclusions: The study provides some compelling evidences supporting the anti-metastatic potential of Yukyung Karne which strongly suggests its possible usage as a promising alternative medicine. Thus, Yukyung Karne may be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to increase their efficacy.

No MeSH data available.


Related in: MedlinePlus

Inhibition of ovarian cancer cell migration and invasion in the presence of Yukyung Karne. a SKOV6 cells were grown in the presence of Paclitaxel (Pac, 10 nM), or different concentrations of Yukyung Karne (YK 50 μg, 100 μg, 200 μg) or PY [Pac (10 nM) + YK (100 μg)] for 24 h and cell invasion was determined by using transwell plates. No. of cells infiltrating through 8 μm pore size filter were stained with crystal violet (20 %), air dried and quantified by microscopy. b SKOV6 cells were grown in the presence of Yukyung Karne (100 μg) and subjected to scratch assay for 6 h or 24 h. Representative images of 3 independent experiments are shown here. All results are shown as mean ± S.D. from five random fields. Statistical significance:*, p <0.05, **, p <0.001
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Fig3: Inhibition of ovarian cancer cell migration and invasion in the presence of Yukyung Karne. a SKOV6 cells were grown in the presence of Paclitaxel (Pac, 10 nM), or different concentrations of Yukyung Karne (YK 50 μg, 100 μg, 200 μg) or PY [Pac (10 nM) + YK (100 μg)] for 24 h and cell invasion was determined by using transwell plates. No. of cells infiltrating through 8 μm pore size filter were stained with crystal violet (20 %), air dried and quantified by microscopy. b SKOV6 cells were grown in the presence of Yukyung Karne (100 μg) and subjected to scratch assay for 6 h or 24 h. Representative images of 3 independent experiments are shown here. All results are shown as mean ± S.D. from five random fields. Statistical significance:*, p <0.05, **, p <0.001

Mentions: Metastasis of ovarian cancer cells from the primary site to the neighboring secondary tissues mainly peritoneum and omentum is the main event in transition of benign to malignant cancer. To elucidate the role of Yukyung Karne in inhibiting the migratory potential of SKOV6 cells, we performed trans-well migration and wound healing assays. A close examination of the invasive potential of Yukyung Karne using transwell insert (Fig. 3a) revealed a significant reduction in the number of infiltrated cells in a dose dependent manner (p <0.001). Further, in wound healing assay (Fig. 3b), Yukyung Karne severely impaired the migratory behavior of SKOV6 cells when compared to untreated cells by 20 % and 80 % at 6 h and 24 h respectively. Taken together these results confirm the inhibitory effect of Yukyung Karne on the migration and invasion potential of SKOV6 cells.Fig. 3


The traditional Tibetan medicine Yukyung Karne exhibits a potent anti-metastatic activity by inhibiting the epithelial to mesenchymal transition and cell migration.

Choedon T, Mathan G, Kumar V - BMC Complement Altern Med (2015)

Inhibition of ovarian cancer cell migration and invasion in the presence of Yukyung Karne. a SKOV6 cells were grown in the presence of Paclitaxel (Pac, 10 nM), or different concentrations of Yukyung Karne (YK 50 μg, 100 μg, 200 μg) or PY [Pac (10 nM) + YK (100 μg)] for 24 h and cell invasion was determined by using transwell plates. No. of cells infiltrating through 8 μm pore size filter were stained with crystal violet (20 %), air dried and quantified by microscopy. b SKOV6 cells were grown in the presence of Yukyung Karne (100 μg) and subjected to scratch assay for 6 h or 24 h. Representative images of 3 independent experiments are shown here. All results are shown as mean ± S.D. from five random fields. Statistical significance:*, p <0.05, **, p <0.001
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4471927&req=5

Fig3: Inhibition of ovarian cancer cell migration and invasion in the presence of Yukyung Karne. a SKOV6 cells were grown in the presence of Paclitaxel (Pac, 10 nM), or different concentrations of Yukyung Karne (YK 50 μg, 100 μg, 200 μg) or PY [Pac (10 nM) + YK (100 μg)] for 24 h and cell invasion was determined by using transwell plates. No. of cells infiltrating through 8 μm pore size filter were stained with crystal violet (20 %), air dried and quantified by microscopy. b SKOV6 cells were grown in the presence of Yukyung Karne (100 μg) and subjected to scratch assay for 6 h or 24 h. Representative images of 3 independent experiments are shown here. All results are shown as mean ± S.D. from five random fields. Statistical significance:*, p <0.05, **, p <0.001
Mentions: Metastasis of ovarian cancer cells from the primary site to the neighboring secondary tissues mainly peritoneum and omentum is the main event in transition of benign to malignant cancer. To elucidate the role of Yukyung Karne in inhibiting the migratory potential of SKOV6 cells, we performed trans-well migration and wound healing assays. A close examination of the invasive potential of Yukyung Karne using transwell insert (Fig. 3a) revealed a significant reduction in the number of infiltrated cells in a dose dependent manner (p <0.001). Further, in wound healing assay (Fig. 3b), Yukyung Karne severely impaired the migratory behavior of SKOV6 cells when compared to untreated cells by 20 % and 80 % at 6 h and 24 h respectively. Taken together these results confirm the inhibitory effect of Yukyung Karne on the migration and invasion potential of SKOV6 cells.Fig. 3

Bottom Line: Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic action remains elusive.The effect of Yukyung Karne on the expression of extracellular matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and western blotting.Thus, Yukyung Karne may be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to increase their efficacy.

View Article: PubMed Central - PubMed

Affiliation: Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.

ABSTRACT

Background: In Traditional Tibetan medicine, Yukyung Karne has been used for the treatment of ovarian cancer. Though Yukyung Karne has been reported to be clinically effective, the molecular mechanism of its anti-metstatic action remains elusive.

Methods: The cytotoxic property of Yukyung Karne was evaluated by crystal violet staining while its ability to induce ceramide production was analyzed by sphingomyelinase assay. The anti-metastatic property was investigated using adhesion, invasion, migration and colony formation assays. The effect of Yukyung Karne on the expression of extracellular matrix components, and epithelial and mesenchymal markers were evaluated by confocal microscopy and western blotting.

Results: Yukyung Karne exhibited a strong anti-metastatic property by significantly reducing the invasion, migration and colony formation ability of ovarian cancer cells. Besides it inhibited the levels of biomarkers involved in epithelial to mesenchymal transition such as down-regulation of vimentin and N-cadherin and up-regulation of epithelial E-cadherin. Yukyung Karne also induced the neutral sphingomyelinase II (nSMNaseII) enzyme activity that is known to hydrolyze sphingomyelins into pro-apoptotic intracellular molecule ceramide.

Conclusions: The study provides some compelling evidences supporting the anti-metastatic potential of Yukyung Karne which strongly suggests its possible usage as a promising alternative medicine. Thus, Yukyung Karne may be used as an anticancer and anti-metastatic agent along with other conventional anticancer therapeutics to increase their efficacy.

No MeSH data available.


Related in: MedlinePlus