Limits...
Gastrointestinal protective efficacy of Kolaviron (a bi-flavonoid from Garcinia kola) following a single administration of sodium arsenite in rats: Biochemical and histopathological studies.

Akinrinde AS, Olowu E, Oyagbemi AA, Omobowale OT - Pharmacognosy Res (2015 Jul-Sep)

Bottom Line: Hydrogen peroxide, reduced glutathione, malondialdehyde (MDA) concentrations as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and myeloperoxidase (MPO) were measured in the remaining portions of the different gastrointestinal tract (GIT) segments.NaAsO2 caused significant increases (P < 0.05) in MDA levels and MPO activity, with significant reductions (P < 0.05) in GST, GPX, CAT and SOD activities in the stomach and intestines.KV significantly reversed the changes (P < 0.05) in a largely dose-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.

ABSTRACT

Background: Arsenic intoxication is known to produce symptoms including diarrhea and vomiting, which are indications of gastrointestinal dysfunction.

Objective: We investigated whether Kolaviron (KV) administration protected against sodium arsenite (NaAsO2)-induced damage to gastric and intestinal epithelium in rats.

Materials and methods: Control rats (Group I) were given a daily oral dose of corn oil. Rats in other groups were given a single dose of NaAsO2 (100 mg/kg; intraperitoneal) alone (Group II) or after pretreatment for 7 days with KV at 100 mg/kg (Group III) and 200 mg/kg (Group IV). Rats were sacrificed afterward and portions of the stomach, small intestine and colon were processed for histopathological examination. Hydrogen peroxide, reduced glutathione, malondialdehyde (MDA) concentrations as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and myeloperoxidase (MPO) were measured in the remaining portions of the different gastrointestinal tract (GIT) segments.

Results: NaAsO2 caused significant increases (P < 0.05) in MDA levels and MPO activity, with significant reductions (P < 0.05) in GST, GPX, CAT and SOD activities in the stomach and intestines. KV significantly reversed the changes (P < 0.05) in a largely dose-dependent manner. The different segments had marked inflammatory cellular infiltration, with hyperplasia of the crypts, which occurred to much lesser degrees with KV administration.

Conclusion: The present findings showed that KV might be a potent product for mitigating NaAsO2 toxicity in the GIT.

No MeSH data available.


Related in: MedlinePlus

Effect of Kolaviron (KV) on malondialdehyde levels in the stomach and intestines of sodium arsenite-treated Wistar rats. Values are expressed as mean ± standard deviation of six rats *P < 0.05 by Student's t-test when the values of group II (Na arsenite) are compared with those of group I (control) **P < 0.05 by Student's t-test when the values of groups III (Na arsenite + 100 mg/kgKV) and IV (Na arsenite + 200 mg/kg KV) are compared with those of group II (Na arsenite)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4471654&req=5

Figure 2: Effect of Kolaviron (KV) on malondialdehyde levels in the stomach and intestines of sodium arsenite-treated Wistar rats. Values are expressed as mean ± standard deviation of six rats *P < 0.05 by Student's t-test when the values of group II (Na arsenite) are compared with those of group I (control) **P < 0.05 by Student's t-test when the values of groups III (Na arsenite + 100 mg/kgKV) and IV (Na arsenite + 200 mg/kg KV) are compared with those of group II (Na arsenite)

Mentions: The protective effect of KV on NaAsO2 -induced lipid peroxidation in stomach and intestines is presented in Figure 2. Although NaAsO2 did not produce significant changes in MDA values when compared to control values, KV pretreatment, followed by the single dose of NaAsO2 produced significant reduction (P < 0.05) in lipid peroxidation, compared to both normal control and NaAsO2 control. This was indicated by reduced MDA level which was more obvious in the stomach and colon. The reduction in MDA was observed to be dose-dependent.


Gastrointestinal protective efficacy of Kolaviron (a bi-flavonoid from Garcinia kola) following a single administration of sodium arsenite in rats: Biochemical and histopathological studies.

Akinrinde AS, Olowu E, Oyagbemi AA, Omobowale OT - Pharmacognosy Res (2015 Jul-Sep)

Effect of Kolaviron (KV) on malondialdehyde levels in the stomach and intestines of sodium arsenite-treated Wistar rats. Values are expressed as mean ± standard deviation of six rats *P < 0.05 by Student's t-test when the values of group II (Na arsenite) are compared with those of group I (control) **P < 0.05 by Student's t-test when the values of groups III (Na arsenite + 100 mg/kgKV) and IV (Na arsenite + 200 mg/kg KV) are compared with those of group II (Na arsenite)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4471654&req=5

Figure 2: Effect of Kolaviron (KV) on malondialdehyde levels in the stomach and intestines of sodium arsenite-treated Wistar rats. Values are expressed as mean ± standard deviation of six rats *P < 0.05 by Student's t-test when the values of group II (Na arsenite) are compared with those of group I (control) **P < 0.05 by Student's t-test when the values of groups III (Na arsenite + 100 mg/kgKV) and IV (Na arsenite + 200 mg/kg KV) are compared with those of group II (Na arsenite)
Mentions: The protective effect of KV on NaAsO2 -induced lipid peroxidation in stomach and intestines is presented in Figure 2. Although NaAsO2 did not produce significant changes in MDA values when compared to control values, KV pretreatment, followed by the single dose of NaAsO2 produced significant reduction (P < 0.05) in lipid peroxidation, compared to both normal control and NaAsO2 control. This was indicated by reduced MDA level which was more obvious in the stomach and colon. The reduction in MDA was observed to be dose-dependent.

Bottom Line: Hydrogen peroxide, reduced glutathione, malondialdehyde (MDA) concentrations as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and myeloperoxidase (MPO) were measured in the remaining portions of the different gastrointestinal tract (GIT) segments.NaAsO2 caused significant increases (P < 0.05) in MDA levels and MPO activity, with significant reductions (P < 0.05) in GST, GPX, CAT and SOD activities in the stomach and intestines.KV significantly reversed the changes (P < 0.05) in a largely dose-dependent manner.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria.

ABSTRACT

Background: Arsenic intoxication is known to produce symptoms including diarrhea and vomiting, which are indications of gastrointestinal dysfunction.

Objective: We investigated whether Kolaviron (KV) administration protected against sodium arsenite (NaAsO2)-induced damage to gastric and intestinal epithelium in rats.

Materials and methods: Control rats (Group I) were given a daily oral dose of corn oil. Rats in other groups were given a single dose of NaAsO2 (100 mg/kg; intraperitoneal) alone (Group II) or after pretreatment for 7 days with KV at 100 mg/kg (Group III) and 200 mg/kg (Group IV). Rats were sacrificed afterward and portions of the stomach, small intestine and colon were processed for histopathological examination. Hydrogen peroxide, reduced glutathione, malondialdehyde (MDA) concentrations as well as activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione S-transferase (GST) and myeloperoxidase (MPO) were measured in the remaining portions of the different gastrointestinal tract (GIT) segments.

Results: NaAsO2 caused significant increases (P < 0.05) in MDA levels and MPO activity, with significant reductions (P < 0.05) in GST, GPX, CAT and SOD activities in the stomach and intestines. KV significantly reversed the changes (P < 0.05) in a largely dose-dependent manner. The different segments had marked inflammatory cellular infiltration, with hyperplasia of the crypts, which occurred to much lesser degrees with KV administration.

Conclusion: The present findings showed that KV might be a potent product for mitigating NaAsO2 toxicity in the GIT.

No MeSH data available.


Related in: MedlinePlus