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Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs.

Omobowale TO, Oyagbemi AA, Oyewunmi OA, Adejumobi OA - Pharmacognosy Res (2015 Jul-Sep)

Bottom Line: However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively.Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals.Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei.

View Article: PubMed Central - PubMed

Affiliation: Departments of Veterinary Medicine and Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.

ABSTRACT

Introduction: The medicinal properties of Azadirachta indica have been harnessed for many years in the treatment of many diseases in both humans and animals.

Materials and methods: Twenty-five apparently healthy dogs weighing between 3 and 8 kg were randomly divided into five groups with five dogs in each group. Ameliorative effect of A. indica on erythrocyte antioxidant status and markers of oxidative stress were assessed. Liver and kidney function tests were also performed.

Results: Pre-treatment with methanolic extract of Azadirachta indica (MEAI) at different doses did not significantly alter the values of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activity in Trypanosoma brucei infection. Although, serum creatinine significantly (P < 0.05) decreased with pre-treatment with 50 mg/kg A. indica, after 2 weeks of T. brucei infection. However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively. Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals. Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei.

Conclusion: From this study, MEAI showed significant ability to attenuate oxidative stress and inflammation during experimental T. brucei infection.

No MeSH data available.


Related in: MedlinePlus

Effect of AI on AST in experimental T. brucei infection. Values are presented as mean ± standard deviation. Superscript (a) Indicates statistically significant when groups II, III, IV and V are compared with group I. Superscript (b) Indicates statistically significant when groups III, IV and V are compared with group II
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Figure 5: Effect of AI on AST in experimental T. brucei infection. Values are presented as mean ± standard deviation. Superscript (a) Indicates statistically significant when groups II, III, IV and V are compared with group I. Superscript (b) Indicates statistically significant when groups III, IV and V are compared with group II

Mentions: Dogs pretreated with MEAI (50, 100 and 200 mg/kg) did not show any significant (P > 0.05) difference in the values of serum ALT, ALP and AST after 1-week of administration [Figures 3–5]. Similarly, also, there was no significant (P > 0.05) difference in the values of ALT, ALP, AST after 2 weeks and 4 weeks of T. brucei infection [Figures 3–5].


Chemopreventive effect of methanolic extract of Azadirachta indica on experimental Trypanosoma brucei induced oxidative stress in dogs.

Omobowale TO, Oyagbemi AA, Oyewunmi OA, Adejumobi OA - Pharmacognosy Res (2015 Jul-Sep)

Effect of AI on AST in experimental T. brucei infection. Values are presented as mean ± standard deviation. Superscript (a) Indicates statistically significant when groups II, III, IV and V are compared with group I. Superscript (b) Indicates statistically significant when groups III, IV and V are compared with group II
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4471651&req=5

Figure 5: Effect of AI on AST in experimental T. brucei infection. Values are presented as mean ± standard deviation. Superscript (a) Indicates statistically significant when groups II, III, IV and V are compared with group I. Superscript (b) Indicates statistically significant when groups III, IV and V are compared with group II
Mentions: Dogs pretreated with MEAI (50, 100 and 200 mg/kg) did not show any significant (P > 0.05) difference in the values of serum ALT, ALP and AST after 1-week of administration [Figures 3–5]. Similarly, also, there was no significant (P > 0.05) difference in the values of ALT, ALP, AST after 2 weeks and 4 weeks of T. brucei infection [Figures 3–5].

Bottom Line: However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively.Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals.Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei.

View Article: PubMed Central - PubMed

Affiliation: Departments of Veterinary Medicine and Physiology, Biochemistry and Pharmacology, University of Ibadan, Ibadan, Nigeria.

ABSTRACT

Introduction: The medicinal properties of Azadirachta indica have been harnessed for many years in the treatment of many diseases in both humans and animals.

Materials and methods: Twenty-five apparently healthy dogs weighing between 3 and 8 kg were randomly divided into five groups with five dogs in each group. Ameliorative effect of A. indica on erythrocyte antioxidant status and markers of oxidative stress were assessed. Liver and kidney function tests were also performed.

Results: Pre-treatment with methanolic extract of Azadirachta indica (MEAI) at different doses did not significantly alter the values of alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase activity in Trypanosoma brucei infection. Although, serum creatinine significantly (P < 0.05) decreased with pre-treatment with 50 mg/kg A. indica, after 2 weeks of T. brucei infection. However, the reduced glutathione (GSH) content of the erythrocyte increased significantly in animals pre-treated with 50 mg/kg and 200 mg/kg of A. indica respectively. Markers of oxidative stress such as malondialdehyde and hydrogen peroxide generated were higher in animals infected with T. brucei with no significant (P >0.05) difference compared to the values obtained in pre-treated animals. Pre-treatment with 100 mg/kg and 200 mg/kg of A. indica significantly (P < 0.05) decreased serum myeloperoxidase activity at 2 weeks post-infection with T. brucei.

Conclusion: From this study, MEAI showed significant ability to attenuate oxidative stress and inflammation during experimental T. brucei infection.

No MeSH data available.


Related in: MedlinePlus