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Formation and abundance of 5-hydroxymethylcytosine in RNA.

Huber SM, van Delft P, Mendil L, Bachman M, Smollett K, Werner F, Miska EA, Balasubramanian S - Chembiochem (2015)

Bottom Line: Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcytidine (f(5)C).Furthermore, we have identified hm(5)C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells.This suggests m(5)C oxidation is a conserved process that could have critical regulatory functions inside cells.

View Article: PubMed Central - PubMed

Affiliation: University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW (UK).

No MeSH data available.


In vivo methylation and subsequent oxidation of cytidine as detected by MS analysis of RNA from the brain of mice fed with l-[methyl-13CD3]methionine. Numbers correspond to the ratio of peak areas of the depicted labelled nucleosides (in grey) against the respective unlabelled nucleosides (in brackets). See Figure S6.1 and Table S6.3 for extracted ion count chromatograms and absolute values obtained thereof, and Table S6.4 for HR-LC-MS/MS data.
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fig03: In vivo methylation and subsequent oxidation of cytidine as detected by MS analysis of RNA from the brain of mice fed with l-[methyl-13CD3]methionine. Numbers correspond to the ratio of peak areas of the depicted labelled nucleosides (in grey) against the respective unlabelled nucleosides (in brackets). See Figure S6.1 and Table S6.3 for extracted ion count chromatograms and absolute values obtained thereof, and Table S6.4 for HR-LC-MS/MS data.


Formation and abundance of 5-hydroxymethylcytosine in RNA.

Huber SM, van Delft P, Mendil L, Bachman M, Smollett K, Werner F, Miska EA, Balasubramanian S - Chembiochem (2015)

In vivo methylation and subsequent oxidation of cytidine as detected by MS analysis of RNA from the brain of mice fed with l-[methyl-13CD3]methionine. Numbers correspond to the ratio of peak areas of the depicted labelled nucleosides (in grey) against the respective unlabelled nucleosides (in brackets). See Figure S6.1 and Table S6.3 for extracted ion count chromatograms and absolute values obtained thereof, and Table S6.4 for HR-LC-MS/MS data.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4471624&req=5

fig03: In vivo methylation and subsequent oxidation of cytidine as detected by MS analysis of RNA from the brain of mice fed with l-[methyl-13CD3]methionine. Numbers correspond to the ratio of peak areas of the depicted labelled nucleosides (in grey) against the respective unlabelled nucleosides (in brackets). See Figure S6.1 and Table S6.3 for extracted ion count chromatograms and absolute values obtained thereof, and Table S6.4 for HR-LC-MS/MS data.
Bottom Line: Herein, we describe an in vivo isotope-tracing methodology to demonstrate that the ribonucleoside 5-methylcytidine (m(5)C) is subject to oxidative processing in mammals, forming 5-hydroxymethylcytidine (hm(5)C) and 5-formylcytidine (f(5)C).Furthermore, we have identified hm(5)C in total RNA from all three domains of life and in polyA-enriched RNA fractions from mammalian cells.This suggests m(5)C oxidation is a conserved process that could have critical regulatory functions inside cells.

View Article: PubMed Central - PubMed

Affiliation: University of Cambridge, Department of Chemistry, Lensfield Road, Cambridge, CB2 1EW (UK).

No MeSH data available.