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Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus.

Thio CL, Yusof R, Ashrafzadeh A, Bahari S, Abdul-Rahman PS, Karsani SA - PLoS ONE (2015)

Bottom Line: These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection.The results presented here will compliment earlier results from the study of late host response.However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia; Drug Design and Development Research Group (DDDRG), University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
The Chikungunya virus (CHIKV) is an arthropod borne virus. In the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. There is limited understanding regarding the underlying molecular mechanisms involved in CHIKV replication and how the virus interacts with its host. In the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early CHIKV infection. Two-dimensional gel electrophoresis was used to analyse and compare the secretome profiles of WRL-68 cells infected with CHIKV against mock control WRL-68 cells. The analysis identified 25 regulated proteins in CHIKV infected cells. STRING network analysis was then used to predict biological processes that may be affected by these proteins. The processes predicted to be affected include signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response. These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection. The results presented here will compliment earlier results from the study of late host response. However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

No MeSH data available.


Related in: MedlinePlus

Secretome networks showing predicted functional linkages between identified proteins.STRING interaction maps were generated using default settings (Medium confidence of 0.4 and 7 criteria for linkage: neighbourhood, gene fusion, co-occurrence, co-expression, experimental evidences, existing databases and textmining). Twenty additional interplay proteins were also added to each network.
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pone.0129033.g004: Secretome networks showing predicted functional linkages between identified proteins.STRING interaction maps were generated using default settings (Medium confidence of 0.4 and 7 criteria for linkage: neighbourhood, gene fusion, co-occurrence, co-expression, experimental evidences, existing databases and textmining). Twenty additional interplay proteins were also added to each network.

Mentions: STRING network analysis of the 20 proteins with different abundance in the secretome of infected cells predicted that 17 were connected by at least one criterion (Fig 4). Gene ontology (GO) enrichment analysis of biological process was further evaluated using the STRING database to identify significant biological processes associated with the network. False discovery rate (FDR) correction was the default statistical method used to determine the significance of each process. Five processes were found to be significant in the predicted secretome network Table 3. Signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response were shown to be involved in the secretome network.


Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus.

Thio CL, Yusof R, Ashrafzadeh A, Bahari S, Abdul-Rahman PS, Karsani SA - PLoS ONE (2015)

Secretome networks showing predicted functional linkages between identified proteins.STRING interaction maps were generated using default settings (Medium confidence of 0.4 and 7 criteria for linkage: neighbourhood, gene fusion, co-occurrence, co-expression, experimental evidences, existing databases and textmining). Twenty additional interplay proteins were also added to each network.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4470940&req=5

pone.0129033.g004: Secretome networks showing predicted functional linkages between identified proteins.STRING interaction maps were generated using default settings (Medium confidence of 0.4 and 7 criteria for linkage: neighbourhood, gene fusion, co-occurrence, co-expression, experimental evidences, existing databases and textmining). Twenty additional interplay proteins were also added to each network.
Mentions: STRING network analysis of the 20 proteins with different abundance in the secretome of infected cells predicted that 17 were connected by at least one criterion (Fig 4). Gene ontology (GO) enrichment analysis of biological process was further evaluated using the STRING database to identify significant biological processes associated with the network. False discovery rate (FDR) correction was the default statistical method used to determine the significance of each process. Five processes were found to be significant in the predicted secretome network Table 3. Signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response were shown to be involved in the secretome network.

Bottom Line: These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection.The results presented here will compliment earlier results from the study of late host response.However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia; Drug Design and Development Research Group (DDDRG), University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
The Chikungunya virus (CHIKV) is an arthropod borne virus. In the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. There is limited understanding regarding the underlying molecular mechanisms involved in CHIKV replication and how the virus interacts with its host. In the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early CHIKV infection. Two-dimensional gel electrophoresis was used to analyse and compare the secretome profiles of WRL-68 cells infected with CHIKV against mock control WRL-68 cells. The analysis identified 25 regulated proteins in CHIKV infected cells. STRING network analysis was then used to predict biological processes that may be affected by these proteins. The processes predicted to be affected include signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response. These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection. The results presented here will compliment earlier results from the study of late host response. However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

No MeSH data available.


Related in: MedlinePlus