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Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus.

Thio CL, Yusof R, Ashrafzadeh A, Bahari S, Abdul-Rahman PS, Karsani SA - PLoS ONE (2015)

Bottom Line: These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection.The results presented here will compliment earlier results from the study of late host response.However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia; Drug Design and Development Research Group (DDDRG), University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
The Chikungunya virus (CHIKV) is an arthropod borne virus. In the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. There is limited understanding regarding the underlying molecular mechanisms involved in CHIKV replication and how the virus interacts with its host. In the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early CHIKV infection. Two-dimensional gel electrophoresis was used to analyse and compare the secretome profiles of WRL-68 cells infected with CHIKV against mock control WRL-68 cells. The analysis identified 25 regulated proteins in CHIKV infected cells. STRING network analysis was then used to predict biological processes that may be affected by these proteins. The processes predicted to be affected include signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response. These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection. The results presented here will compliment earlier results from the study of late host response. However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

No MeSH data available.


Related in: MedlinePlus

Expanded views showing the location of differentially expressed protein spots on the mock control and CHIKV-infected WRL-68 secretome gels.Expanded view of five sections—I, II, III and IV, showing differentially expressed proteins between A: mock control and B: CHIKV-infected 2DE gels. Spots 1 to 9 refer to up-regulated protein spots, whereas DS10 to DS34 refer to down-regulated protein spots. Five biological replicates per group (n = 5) were used in the analysis.
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pone.0129033.g003: Expanded views showing the location of differentially expressed protein spots on the mock control and CHIKV-infected WRL-68 secretome gels.Expanded view of five sections—I, II, III and IV, showing differentially expressed proteins between A: mock control and B: CHIKV-infected 2DE gels. Spots 1 to 9 refer to up-regulated protein spots, whereas DS10 to DS34 refer to down-regulated protein spots. Five biological replicates per group (n = 5) were used in the analysis.

Mentions: A representative 2DE gel of the secretome is provided in Fig 2. An expanded view showing spots with different abundance between the secretome of mock control and CHIKV infected cells is shown in Fig 3. Five biological replicates (five individual cultures) were used for analysis of each group. More than 1,300 individual protein spots were resolved by 2DE. Quantitative analysis of normalized spot volume revealed 34 spots with significant differences in abundance (at least 1.5 fold difference and p<0.05, as determined by ANOVA and Student’s t-test). The majority of the protein spots were found to be decreased in abundance. The identification of these proteins was then performed by MALDI-TOF MS/MS. A list of identified proteins is shown in Table 1. A major proportion were found to be involved in transport as well as in immune and defense response (25.00% for both).


Differential Analysis of the Secretome of WRL68 Cells Infected with the Chikungunya Virus.

Thio CL, Yusof R, Ashrafzadeh A, Bahari S, Abdul-Rahman PS, Karsani SA - PLoS ONE (2015)

Expanded views showing the location of differentially expressed protein spots on the mock control and CHIKV-infected WRL-68 secretome gels.Expanded view of five sections—I, II, III and IV, showing differentially expressed proteins between A: mock control and B: CHIKV-infected 2DE gels. Spots 1 to 9 refer to up-regulated protein spots, whereas DS10 to DS34 refer to down-regulated protein spots. Five biological replicates per group (n = 5) were used in the analysis.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470940&req=5

pone.0129033.g003: Expanded views showing the location of differentially expressed protein spots on the mock control and CHIKV-infected WRL-68 secretome gels.Expanded view of five sections—I, II, III and IV, showing differentially expressed proteins between A: mock control and B: CHIKV-infected 2DE gels. Spots 1 to 9 refer to up-regulated protein spots, whereas DS10 to DS34 refer to down-regulated protein spots. Five biological replicates per group (n = 5) were used in the analysis.
Mentions: A representative 2DE gel of the secretome is provided in Fig 2. An expanded view showing spots with different abundance between the secretome of mock control and CHIKV infected cells is shown in Fig 3. Five biological replicates (five individual cultures) were used for analysis of each group. More than 1,300 individual protein spots were resolved by 2DE. Quantitative analysis of normalized spot volume revealed 34 spots with significant differences in abundance (at least 1.5 fold difference and p<0.05, as determined by ANOVA and Student’s t-test). The majority of the protein spots were found to be decreased in abundance. The identification of these proteins was then performed by MALDI-TOF MS/MS. A list of identified proteins is shown in Table 1. A major proportion were found to be involved in transport as well as in immune and defense response (25.00% for both).

Bottom Line: These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection.The results presented here will compliment earlier results from the study of late host response.However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia; Drug Design and Development Research Group (DDDRG), University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
The Chikungunya virus (CHIKV) is an arthropod borne virus. In the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. There is limited understanding regarding the underlying molecular mechanisms involved in CHIKV replication and how the virus interacts with its host. In the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early CHIKV infection. Two-dimensional gel electrophoresis was used to analyse and compare the secretome profiles of WRL-68 cells infected with CHIKV against mock control WRL-68 cells. The analysis identified 25 regulated proteins in CHIKV infected cells. STRING network analysis was then used to predict biological processes that may be affected by these proteins. The processes predicted to be affected include signal transduction, cellular component and extracellular matrix (ECM) organization, regulation of cytokine stimulus and immune response. These results provide an initial view of CHIKV may affect the secretome of infected cells during early infection. The results presented here will compliment earlier results from the study of late host response. However, functional characterization will be necessary to further enhance our understanding of the roles played by these proteins in the early stages of CHIKV infection in humans.

No MeSH data available.


Related in: MedlinePlus