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Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803.

Zhang L, Selão TT, Selstam E, Norling B - PLoS ONE (2015)

Bottom Line: Carotenoid analysis of different membrane subfractions in Synechocystis sp.PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids.Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.

ABSTRACT
The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.

No MeSH data available.


Carotenoid distribution in Synechocystis membranes.Total carotenoid amount in purified PM1, PM2 and TM (A). Relative amount of different carotenoids in each membrane (B). Data represent means ± standard deviations for carotenoids in membranes from three independent cultures. For results of statistical analysis, please refer to S2 Table.
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pone.0130904.g002: Carotenoid distribution in Synechocystis membranes.Total carotenoid amount in purified PM1, PM2 and TM (A). Relative amount of different carotenoids in each membrane (B). Data represent means ± standard deviations for carotenoids in membranes from three independent cultures. For results of statistical analysis, please refer to S2 Table.

Mentions: Carotenoid extraction from PM1, PM2 and TM of wild type cells was carried out with two different extraction methods. Similar results were obtained with both methods and included in the mean of each independent sample. Substantial differences were observed between different membranes, with the total content of carotenoids on a protein basis being highest in PM1, while in TM and PM2 there were only 16% and 11% respectively of the PM1 carotenoid content (Fig 2A). The carotenoid composition analyses revealed that each membrane has the same kind of carotenoids though in different proportions (Fig 2B). For instance, in PM1, 54% of the total carotenoid content is comprised of β-carotene, with myxoxanthophyll, zeaxanthin, echinenone and other minor carotenoids. This character is similar to that of TM (the most abundant membrane in Synechocystis), which also contains mostly β-carotene, albeit in a lower concentration (42%). PM2, on the other hand, has a different composition in comparison to other membranes, with 41% of myxoxanthophyll, 25% of zeaxanthin and 20% of β-carotene.


Subcellular Localization of Carotenoid Biosynthesis in Synechocystis sp. PCC 6803.

Zhang L, Selão TT, Selstam E, Norling B - PLoS ONE (2015)

Carotenoid distribution in Synechocystis membranes.Total carotenoid amount in purified PM1, PM2 and TM (A). Relative amount of different carotenoids in each membrane (B). Data represent means ± standard deviations for carotenoids in membranes from three independent cultures. For results of statistical analysis, please refer to S2 Table.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4470828&req=5

pone.0130904.g002: Carotenoid distribution in Synechocystis membranes.Total carotenoid amount in purified PM1, PM2 and TM (A). Relative amount of different carotenoids in each membrane (B). Data represent means ± standard deviations for carotenoids in membranes from three independent cultures. For results of statistical analysis, please refer to S2 Table.
Mentions: Carotenoid extraction from PM1, PM2 and TM of wild type cells was carried out with two different extraction methods. Similar results were obtained with both methods and included in the mean of each independent sample. Substantial differences were observed between different membranes, with the total content of carotenoids on a protein basis being highest in PM1, while in TM and PM2 there were only 16% and 11% respectively of the PM1 carotenoid content (Fig 2A). The carotenoid composition analyses revealed that each membrane has the same kind of carotenoids though in different proportions (Fig 2B). For instance, in PM1, 54% of the total carotenoid content is comprised of β-carotene, with myxoxanthophyll, zeaxanthin, echinenone and other minor carotenoids. This character is similar to that of TM (the most abundant membrane in Synechocystis), which also contains mostly β-carotene, albeit in a lower concentration (42%). PM2, on the other hand, has a different composition in comparison to other membranes, with 41% of myxoxanthophyll, 25% of zeaxanthin and 20% of β-carotene.

Bottom Line: Carotenoid analysis of different membrane subfractions in Synechocystis sp.PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids.Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.

View Article: PubMed Central - PubMed

Affiliation: School of Biological Sciences, Nanyang Technological University, Singapore.

ABSTRACT
The biosynthesis pathway of carotenoids in cyanobacteria is partly described. However, the subcellular localization of individual steps is so far unknown. Carotenoid analysis of different membrane subfractions in Synechocystis sp. PCC6803 shows that "light" plasma membranes have a high carotenoid/protein ratio, when compared to "heavier" plasma membranes or thylakoids. The localization of CrtQ and CrtO, two well-defined carotenoid synthesis pathway enzymes in Synechocystis, was studied by epitope tagging and western blots. Both enzymes are locally more abundant in plasma membranes than in thylakoids, implying that the plasma membrane has higher synthesis rates of β-carotene precursor molecules and echinenone.

No MeSH data available.