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Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus

Determination of two parameters, time to 50% mortality (A) and the logistic rate (B), in the logistic models describing the dynamics of viable Puccinia striiformis f. sp. tritici over time for wheat cultivars with different levels of winter hardness tested at various low temperatures.
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pone.0130691.g008: Determination of two parameters, time to 50% mortality (A) and the logistic rate (B), in the logistic models describing the dynamics of viable Puccinia striiformis f. sp. tritici over time for wheat cultivars with different levels of winter hardness tested at various low temperatures.

Mentions: The temporal dynamics of viable Pst at a given temperature was well described by a logistic model with two parameters: rate of Pst mortality (r) and time to 50% mortality (m). This model explained more than 99% of the total variability in average viable Pst over time for all 30 datasets (five temperatures × six cultivars). Both r and m were related to temperature though the relationship of m with temperature (Fig 8A) was stronger than that of r with temperature (Fig 8B). Overall, m decreased with decreasing temperature; differences in m among cultivars were primarily at 0°C and -5°C. In contrast, there were large differences in r across all test temperatures; however, there appeared to be no discernable pattern of r in relation to either of cultivar or winter hardiness.


Effect of Low Temperature and Wheat Winter-Hardiness on Survival of Puccinia striiformis f. sp. tritici under Controlled Conditions.

Ma L, Qiao J, Kong X, Zou Y, Xu X, Chen X, Hu X - PLoS ONE (2015)

Determination of two parameters, time to 50% mortality (A) and the logistic rate (B), in the logistic models describing the dynamics of viable Puccinia striiformis f. sp. tritici over time for wheat cultivars with different levels of winter hardness tested at various low temperatures.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4470655&req=5

pone.0130691.g008: Determination of two parameters, time to 50% mortality (A) and the logistic rate (B), in the logistic models describing the dynamics of viable Puccinia striiformis f. sp. tritici over time for wheat cultivars with different levels of winter hardness tested at various low temperatures.
Mentions: The temporal dynamics of viable Pst at a given temperature was well described by a logistic model with two parameters: rate of Pst mortality (r) and time to 50% mortality (m). This model explained more than 99% of the total variability in average viable Pst over time for all 30 datasets (five temperatures × six cultivars). Both r and m were related to temperature though the relationship of m with temperature (Fig 8A) was stronger than that of r with temperature (Fig 8B). Overall, m decreased with decreasing temperature; differences in m among cultivars were primarily at 0°C and -5°C. In contrast, there were large differences in r across all test temperatures; however, there appeared to be no discernable pattern of r in relation to either of cultivar or winter hardiness.

Bottom Line: The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time.The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness.Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Taicheng Road 3, Yangling 712100, China.

ABSTRACT
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases of wheat worldwide. Understanding the survival of Pst during the overwintering period is critical for predicting Pst epidemics in the spring. Real-time quantitative PCR (qPCR) methods quantifying Pst DNA and RNA (cDNA) were developed and compared for the ability to quantify viable Pst in leaf tissues. Both qPCR of DNA and RNA can provide reliable measurement of viable Pst in plant tissues prior to the late sporulation stage for which qPCR of DNA gave a much higher estimate of fungal biomass than qPCR of RNA. The percentage of Pst biomass that was viable in detached and attached leaves under low temperatures decreased over time. Pst survived longer on attached leaves than on detached leaves. The survival of Pst in cultivars with strong winter-hardiness at 0°C and -5°C was greater than those with weak winter-hardiness. However, such differences in Pst survival among cultivars were negligible at -10, -15 and -20°C. Results indicated that Pst mycelia inside green leaves can also be killed by low temperatures rather than through death of green leaves under low temperatures. The relationship of Pst survival in attached leaves with temperature and winter-hardiness was well described by logistic models. Further field evaluation is necessary to assess whether inclusion of other factors such as moisture and snow cover could improve the model performance in predicting Pst overwintering potential, and hence the epidemic in spring.

No MeSH data available.


Related in: MedlinePlus